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Results (1) The forward and reverse subtracted cDNA libraries of different metastastic potential large cell lung cancer cell lines were successfully constructed;(2) With blue-white colony and dot blot, 307 positive clones in the forward subtracted library and 78 positive clones in the reverse subtracted library were obtained;(3) 55 clones were successfully sequenced in the forward subtracted library. Homolog analysis confirmed 23 differential expression segments, which were similar to the human genes already known, including NME2, NPM1, MT 2A, HSPE1, TAFIA, EPRS, PX19 and EIF3S9 et al;(4) 31 clones were successfully sequenced in the reverse subtracted library. Homolog analysis confirmed 16 differentially expressed segments. 15 of them were similar to the human genes already known, including ANXA2, TUBB, PKN2, GNAS, EEF1A1, SSR2 and RPLPO et al. Only one segment had partial homology to known human genes. This segment was supposed to be the new EST segments which might not have been cloned.

结果(1)成功构建人大细胞肺癌高低转移株差异表达基因正向消减cDNA文库和反向消减cDNA文库；(2)经蓝白菌落筛选和斑点杂交，正向消减文库获得307个阳性克隆，反向消减文库获得78个阳性克隆；(3)正向消减文库挑选55个克隆成功测序，经同源性分析最终确定差异表达基因片段23个与已知人类全长基因具有很高相似性(95％～100％)，这些基因包括NME2、NPM1、MT2A、HSPE1、TAFlA、EPRS、PX19和EIF3S9等；(4)反向消减文库挑选31个克隆成功测序，经同源性检索和比对最终确定差异表达基因片段16个，其中15个与人类全长基因具有很高相似性(95％～100％)，包括ANXA2、TUBB、PKN2、GNAS、EEF1A1、SSR2和RPLPO等基因。1个片段和己知人类基因仅有部分相似性，表明它可能是未被克隆的人类基因EST片段。

It's known to us that Walt Disney is famous／well-known／known for his cartoon characters.

我们都知道沃尔特·迪斯尼是因为他的卡通人物而著名。

Gibbsite from which, also known as the aluminum water of oxygen stone components for the Al 3, monoclinic, usually Species Flake collectivity; A Diaspor (diaspore; Empholite) also known as hard water with aluminum components α- AIO, the regular - Fe, Mn, rhomboidal, sustained Species Flake collectivities hardness (6-7); a water boehmite, also known as the mixing aluminum components γ- AlO, the rhomboidal appearing Aphanitic block or colloidal.

密度2-3。其中三水铝石又称水铝氧石，成分为Al3，单斜晶系，通常呈细鳞片状集合体；一水硬铝（diaspore；empholite）又称硬水铝矿，成分α-AIO，常含Fe、Mn，斜方晶系，多呈细鳞片状集合体，硬度大（6-7）；一水软铝石又称勃姆铝矿，成分γ-AlO，斜方晶系，常呈隐晶质块体或胶态。

If, on the role of epigene, would change his instantaneous and shapes and sizes, and when epigene stops, the paper will revert to the original shape and size, this kind of process is known as minyet elastic deformation; when paper was epigene at the time of a recurring interval between defending the changed his shape and size, epigene falls out of scope when you slow down and revert to the original shape and size, this kind of process is known as anelasticity; scrapped when epigene, paper or maintain in force the shape and size of deformation of the State, known as the plastic deformation.

如在边辛的功用下，会瞬时不天保留自己的轮廓和尺寸，而当边辛罢手时，纸张又会复兴到本来的轮廓和尺寸，这栽变形的功程称为敏不不不弹性变形；当纸张施到边辛时在一按期捍间间隔边保留了自己的轮廓和尺寸，边辛罢手功用时则匆匆不天复兴到本来的轮廓和尺寸，这栽变形的功程称为滞不不不弹性变形；当边辛裁撤之后，纸张还是保留在施边辛功用时变不败的轮廓与尺寸变形的状况时，称为不不弹性变形。

Tongue tie is too short, so that should not extend extraoral tongue, the impact of the flexible tongue movement, so the impact of the larger aspects of language development, commonly known as "大舌." 2, these are caused by organic disease transformer ambiguous words; There is also a non-organic ambiguous words, 2-year-old children with limited vocabulary; but would like to use language to express the results difficult to say if a series of adults, medically known as the "insinuations", also known as the "language disorder."

舌系带过短，使舌不能伸出口外，影响舌头的灵活运动，所以对语言发育方面影响较大，俗称&大舌头&。2、上述属于器质性病变压器造成的说话不清；还有一种非器质性的说话不清，2岁小儿词汇有限；但又很想用语言来表达，结果说出一连串成人难懂的话，医学上称之为&隐语&，又称&乱语&。

Mylabris phalerata, Meloidae family of Coleoptera, has been known since antiquity to produce a potent toxic defensive agent, known as cantharidin (exo,exo-2,3-dimethyl-7-oxabicyclo [2.2.1] heptane-2,3- dicarboxylic anhydride), but well-known as Spanish Fly. In the past, Cantharidin has used as anticancer, herbicidal, pesticide and treatment of hepatic carcinoma and molluscum contagiosum activities.

斑蝥为翘翅目芫青科斑芫青属的昆虫，主要成分为斑蝥素(Cantharidin； exo，exo-2，3-Dimethyl- 7-oxabicyclo [2，2，1] heptane-2，3-dicarboxylic anhydride)，研究中指出斑蝥素具有发泡、抗肿瘤的作用，或当作除草剂、杀虫剂、毛发生长刺激剂，目前临床上则用於治疗原发性肝癌及传染性疣。

In this paper we assume the expected returns of the risky and risk-free assets as well as the covariance matrix of the risky assets are not exactly known, but defined by some known elements, which form the vertices of this polytope. Robust optimal portfolio problems are proposed in the market with and without risk-free asset. And the analytical solutions are given out when the covariance matrix of the risky assets are exactly known.

本文假设风险资产和无风险资产的收益以及风险资产的协方差矩阵不是完全确定的，其相关参数属于某个已知的凸多面体，分别在市场不存在和存在无风险资产的情况下定义了稳健最优投资组合问题，并在协方差矩阵确定的情况下给出了问题的解析解。

What can be described is the known, and the freedom from the known can come into being only when there is a dying every day to the known, to the hurts, the flatteries, to all the images you have made, to all your experiences - dying every day so that the brain cells themselves become fresh, young, innocent.

你必须每天都能死于一切已知的创伤、荣辱以及自制的意象和所有的经验，如此你才能从已知中解脱。每天都大死一番，脑细胞才会变得清新、年轻而单纯。

Li Hu, formerly known Wuli Lake, also known as Chatham Lake Five or lake, after Lake, such as the shape of a gourd dipper, so also known as Li Hu (calabash gourd dipper words intended to mean).

蠡湖原名五里湖，又名漆湖或小五湖，后因湖形状如一只葫芦瓢，所以又名蠡湖。

Automotive snorkels Mei-called cantilever handling devices, referred to the end of car plate; Tipper roof closed system known as dump trucks cap, dump trucks automatically open the cap, is all over urban management and sanitation sector in order to improve the region The urban environment, and regulate the transport and Zhatu Daofang, in particular to ask the soil transport vehicles to install the closed roof; regulating cargo storage warehouse known as the Taiwan-conditioning, hydraulic boarding palanquin, the goal is to regulate the storage bed The high level reached with the different types of cargo compartment of a smooth convergence to facilitate loading and unloading; fixed-take-off and landing platform known as shear platform, is a goods from low to high or the removal from height moving to lower the hydraulic Auxiliary handling machinery.

汽车液压升降尾板又叫悬臂式装卸装置，简称汽车尾板；自卸车顶盖密闭系统又叫泥头车顶盖、泥头车自动开启盖，是各地城管和环卫部门为了改善本地区的城市环境，规范渣土的运输与倒放，特别地要求泥土运输车辆必须安装的密闭式顶盖；仓库调节货台又叫仓库调节板、液压登车轿，目的是通过调节仓库货台的高度达到与各类高低不同的货运车厢的顺利衔接，方便装卸；固定式升降平台又叫剪式平台，是一种将货物从低处搬运至高处或从高处搬运至低处的液压式辅助搬运机械。

Metrorrhagia is also gynaecological problems of common diseases.

崩漏是妇科常见病也是疑难症。

WordFTP gives you the convenience and timesaving you need.

wordftp让您的方便和省时您所需要的。