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Compared with classical cytotoxic T lymphocytes , NK cells are superior in the following aspects: NK cells are among the first cell types to be activated after intracellular pathogen stimuli, the activity of NK cells peaked between 1 to 3 days, retained during the first 7-10 days, and the T cells response emerged only after 7 days when the NK cell responses begin to decline; though comprising of only 10%-15% of peripheral lymphocytes, NK cells mobilize most of the cell populations in a strong and high level response to different invasions, and act as key factors at early defense before a specific immune response could mount, while only certain clones were involved in T cell response; NK cells kill virus-infected or malignant transformed cells without pre-sensitization and without restriction by major histocompatibility antigen, which is usually a mechanism of immune escape to T cell recognition by virus-infected or tumor cells, thus NK cell in complementary with T cells play crucial roles in tumor and virus eradication.

NK细胞以其强大的细胞毒活性为特征,与细胞毒性T细胞相比,NK细胞具有如下特点:NK细胞虽然只占外周血淋巴细胞的10-15%,但是对刺激性因素产生应答的过程十分迅速,一次可以调动大多数细胞共同参与,而不是几个克隆的活化,免疫应答的强度高;病毒感染或恶性转化细胞的一个主要特征是细胞表达的MHCⅠ类分子下降或消失,并借助这一机制逃避特异性T细胞应答的识别,NK细胞介导的杀伤活性无需抗原刺激,不受MHCⅠ分子表达的限制,从而与T细胞应答互为补充发挥免疫防御的功能;NK细胞的免疫活性可以被多种细胞因子上调,其中IFNα,IL-2,IL-12,IL-15和IL-18具备更强的作用;NK细胞具有强大的细胞因子分泌功能,对于启动T细胞特异性应答必不可少。

With the use of IgY-based ELISA method, we found that 15%,45%,90% and 100% of the slightly infected mice (5 cercarie per mouse) turned to be negative by detection of circulating SEA when treated by Praziquantel at 2, 4, 6 and 12 months postinfection, respectively; For the moderately infected mice (15 cercarie per mouse), SEA was undetectable in 5%,25%,40% and 70% of mice respectively after treatment at 2, 4, 6 and 12-months postinfection; With the same treatment, 0%,10%,30% and 60% of the heavily infected mice (35 cercarie per mouse) turned negative of SEA detection.

轻度感染(感染尾蚴5条/只小鼠)治疗后2个月,4个月,6个月,12个月的转阴率为15%,45%,90%,100%;中度感染组(感染尾蚴15条/只小鼠)治疗后2个月, 4个月, 6个月,12个月的转阴率分别为5%,25%,40%,70%;重度感染组(感染尾蚴35条/只小鼠)治疗后2个月, 4个月, 6个月,12个月的转阴率分别为0%,10%,30%,60%。

Results: The normal epithelial cells were intactly lined with microvilli, and lesions on the surface of Ct infected cells were showed by SEM. The expression of actin was obvious throughout the cytoplasm of uterine epithelial cells but appeared to be most intense along the apical plasma membrane on day 4, 5 and 6 of pregnancy. On the day 7, levels of actin had increased along the apical surface. The expression of actin in the infected group was weaker than that in of the normal group.

结果:SEM示对照组子宫腔可见有微绒毛的分泌细胞;实验组腔上皮细胞表面有的可见破损,微绒毛少见;免疫组化结果显示荧光强度主要定位于腔上皮细胞胞质/胞膜,妊娠第4,5,6天沿胞质顶部分布较密集,而底部较弥散;妊娠第7天顶部强度有所增加;感染组的荧光强度均弱于相应正常组,在妊娠第5天存在显著性差异(p.05)。

The HPV positivity amongst the group of women with no cervical abnormality was 43%, while that in women with cervical dysplasia and cervical cancer was 81.2% PCR products from 89 HPV positive cases were genotyped by LiPA, of which 49 cases were single HPV infected concerning 11 different HPV types and the commonest type was HPV-16, occurring in 25(51%) of all single HPV infected cases. The other 40 specimens contained multiple HPV types comprising 16 different HPV types. Although double infections(n=25) prevailed, triple(n=9),quadruple(n=3) and quintuple(n=3) infections were also found. PCR products of four HPV positive cases failed to be classified by LiPA. Sequencing analysis revealed that these 4 cases were HPV-66 which was not included in LiPA probes. Follow-up data of 12 cases confirmed the HPV genotyping results. Conclusion The LiPA for HPV genotyping is easy to perform.

结果 155例妇女子宫颈刮片中HPV DNA检出率为60%,其中在宫颈细胞学检查正常或仅有炎症病变的妇女中HPV DNA阳性率为43%,而在宫颈细胞增生不良或宫颈细胞癌的妇女其阳性率为81.2%。93例HPV阳性的标本中,89例利用LiPA成功地作出了基因分型,其中单独一型HPV感染为49例,涉及11种不同基因型HPV,最常见的型别为HPV 16,占单独感染的51%(25/49);另40例为不同型别HPV重叠感染,涉及所有16种HPV基因型别,二重感染占主导地位(n=25),同时也检测出其它多重感染。4例HPV阳性的标本应用LiPA未能作出基因分型,DNA序列分析显示这4例为HPV 66感染,此型别不包括在LiPA所标记的探针内。12例随诊资料显示了LiPA的物异性及高度的重复性。

The EC_(50) of QKL against the formation of syncytia of MT-2 induced by HIV-1_ was 1/198.02 with a SI of 5.36. By detecting the survival rate of co-culturing H9/HIV-1_ cells and MT-2 cells or MT-2 cell direct infected by HIV-1_ QKL was found to be protective to cells. The EC_(50) was 1/166.67 and 1/144.93 with SI of 4.51 and 3.92 respectively. The EC_(50) of QKL against P24 antigen production was 1/175.44 with SI of 4.75. The drug serum of QKL was also found to be effective to inhibit the cell fusion and protect cells infected by HIV-1_.

结果:QKL对H9/HIV-1_细胞和MT-2细胞的CC_(50)分别为1/50.76和1/36.97;抑制H9/HIV-1_细胞和MT-2细胞早期融合的EC_(50)=1/235.29,SI=6.36;抑制HIV-1_细胞诱导MT-2细胞形成合胞体的EC_(50)=1/198.02,SI=5.36;H9/HIV-1_细胞和MT-2细胞混合培养和HIV-1_感染MT-2细胞时,QKL保护病毒感染细胞免于死亡的EC_(50)分别为1/166.67和1/144.93,SI分别为4.51和3.92;抑制p24抗原产生的EC_(50)=1/175.44,SI=4.75;QKL药物血清也可抑制H9/HIV-1_细胞和MT-2细胞的早期融合,保护细胞免于死亡。

Hepatitis B can also be spread by unprotected sexual contact with an infected person; unsterile instruments that may be used in acupuncture, ear and body piercing, or tattooing; illicit injection drug use; and by sharing personal items like toothbrushes or shaving razors with an infected person.

乙型肝炎亦可通过下列途径传播﹕与被感染者从事无防护性行为;针灸、穿耳洞或身体穿刺或刺青时使用未消毒器械;非法注射毒品;及与受感染者共享牙刷或剃刀等个人用品。

Two different signals, whichwere brown and blue, reacted with the substrate (DAB and NBT/BCIP) separately, could be observed in the WSSV infected haemocyte. The result showed that in the lightly infected tissues infected haemocytes predominated in all infected cells. However in the heavily infected tissues the number of normal haemocyte decreased obviously and infected cell with strong positive intensity increased, and cell debris and tissue necrosis were observed.

应用这种双重免疫组化方法观察了被WSSV感染的血细胞在几种组织中的分布情况,发现在轻度感染组织中被侵染的血细胞在所有被感染细胞中占绝大多数,而在高度感染组中血细胞数目明显减少,细胞变形,出现许多的细胞碎片,同时被病毒感染的细胞数目增多,染色强度增强,组织坏死严重。

The NS1 protein is produced in large quantities in infected cells, but it is not packaged in the virion. Since killed vaccines for influenza are primarily made with whole virions, a differential antibody response can be seen between naturally infected and vaccinated animals.

NS1蛋白可以在受病毒感染的细胞中大量产生,但是却不会被包装到病毒颗粒中去;由于灭活禽流感病毒疫苗的成分主要是完整的病毒颗粒,因而免疫接种后机体产生的抗体谱会有别于禽流感病毒感染。

I must admit that I may have been infected with society's prejudices and predilections and attributed them to God; that Jesus' call to love one another might demand a different conclusion; and that in years hence I may be seen as someone who was on the wrong side of history.

我必须承认,我也许一直受到社会偏见和偏好的影响,并将它们归因于上帝;耶稣号召我们互相爱护,但也许会有不同的结局出现;因此几年之后,我也许会站在历史上错误的一边。

Workers who are infected with blood borne pathogens must be protected from any discrimination or stigmatisation by co-workers, unions, employers or clients.

被血液病原体感染的工人必须被保护免受来自同事、团体、雇主和顾客的任何歧视或诋毁。

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This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

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