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Results: The OECs could be purified to about 80 percent purity by the method, identified by immunofluorescence of GFAP and P75 with hoechst; there were many fusiform, bipolar, tripolar cells and a few multipolar cells under a phase contrast microscope.

结果:通过差速贴壁法纯化,可获得纯度约为80%的嗅鞘细胞,并通过GFAP免疫荧光及P75和hoechst双重免疫荧光鉴定;相差显微镜下细胞形态以梭形、双极及三极细胞为主,有少量的多极细胞。

Gastric varices in patients with portal hypertension should be correctly identified.

门脉高压病人的胃静脉曲张应该可以正确地辨认。

The appendix was considered to be the most common site of carcinoid tumor. We retrospectively reviewed 8,961 appendectomies in our hospital from 1995 to 2005, and 12 patients with appendiceal carcinoid tumors were identified.

在过去阑尾被认为是类癌最好发的位置,我们回溯性收集了从1995到2005年在我们医院共施行了8961个阑尾切除手术,其中共有12例阑尾类癌,发生率占0.13%,略低於过去文献报告0.3-0.9%的发生率。

Because it is a single dominant gene with identified DNA markers, it can be readily introduced into susceptible commercial soybean varieties by backcrossing using marker-assisted selection.

因为这是一个DNA标记已经得到确认的单个显性基因,因此完全可以通过标记协助选择以逆代杂交的方式将它植入易受侵袭的商品大豆中。

Though commingled with other funds for investment purposes, your fund will be separately identified in the Foundation's records.

虽然由於投资的目的与其他资金合并在一起,但是你的资金将在基金会的记录里会被单独地确定。

In Part 1 of our research, BGSCs were sorted through immunomagnetic beads marking by CD133 and cultured in vitro, and character as a stem cell was identified by stem cell markers (CD133 and Nestin) and differentiated cell markers [ microtubule-associated protein 2(MAP2), glial acidic fibrillary protein and myelin basic protein] , ultrastructure observing with electron microscopeand engrafting to severe combined immunodeficiency mice for tumorigenesis test. The results were as following: Only a small subset of CD133+ glioma cells in glioma cell lines and fresh specimens from various pathologic grade could express stem cell markers CD133 and Nestin, view ultrastructure of a stem cell and be capacity of serial passage in culture. These CD133+ cells possese a marked capacity for multipotent differentiation and could differentiate into tumor cells expressing MAP2,β-TubulinⅢ, GFAP and MBP; When engrafted into SCID mice, they can generate and form tumors that phenotypically resembl the tumor from the patient.

在本课题中,在第一部分实验中采用以CD133为标志的免疫磁珠法从人脑胶质瘤组织和细胞株中分离脑胶质瘤干细胞并进行体外培养,通过免疫荧光技术检测干细胞标志物CD133、Nestin,诱导分化后检查分化细胞标志物MAP2、GFAP、MBP以及电镜超微结构观察和移植SCID鼠致瘤实验,对其干细胞特性加以鉴定,得到如下结果:不同病理分级的新鲜胶质瘤标本和胶质瘤细胞株中存在一小部分CD133+的胶质瘤细胞,能表达干细胞的标志物CD133和Nestin,符合干细胞的超微结构特点,体外培养能连续传代;具有多向分化潜能:诱导分化后能产生MAP2、β-TubulinⅢ、GFAP、MBP染色阳性的细胞;移植SCID鼠后能形成与亲本肿瘤表型一致的移植瘤。

Using this model three competence-deficient pneumococci, which was identified by PCR and transformation experiment, were constructed. It is found different strain has different bacterial optimal density for being induced into competence. The optimal density for strains 1, 2 and 22 was 0.1, 0.08and 0.07 respectively. The optimal condition for transformation is c plus y culture at pH8.0. In c plus y culture at pH6.8, which restrained pneumococci forming transformation naturally, pneumococci could be induced to form transformation by CSP.It is concluded that we could induce different S.pneumoniae by CSP into competence in their optimal density in laboratory. 2. It was discussed the relationship between pneumococcal transformation formation and its opportunistic pathogenesis. Comparing their virulence with that of parental strains by challenging mice intraperitoneally and adhering to ECV-304 cell respectively.

结果表明不同肺炎链球菌株的转化最适菌密度不同,1、2和22号菌株的最适转化密度分别为0.1、0.08和0.07;用于转化的CTM培养基最适为pH 8.0的C+Y培养基;在自然转化下不能诱导转化发生的pH 6.8的C+Y培养基中,使用CSP诱导,也能促使细菌发生转化,只是转化效率较低;结论:实验室条件下,不同细菌在一定菌密度时可利用CSP诱导肺炎链球菌成为感受态发生转化。2、为探讨肺炎链球菌转化对毒力表达的影响,本研究分别通过体外粘附人脐静脉血管内皮细胞ECV-304和体内小鼠毒力实验观察1、2和22号肺炎链球菌和其转化缺陷菌株1t、2t和22t毒力的差异。

False positive cases were found to contain sounds that simulate wheezes, such as background noises with high frequencies or strong noises from the throat that could be heard and identified without a stethoscope.

假阳性病例可闻及似哮鸣音的声音,如不用听诊器也可听到的背景噪音、喉咙发出的高频率强烈噪声。

First, this paper presents three girders model with double cable planes which main girder and side girder is connected by rigid arms, analyzes the dynamic characteristic of Yonghe cable-stayed bridge and obtains its natural vibration modes (i.e., frequency, damping values and the mode shapes ) by ANLSIS structural analysis program and uses these parameters as dynamic dactylogram of the intact case of cable-stayed bridge. Using the passage of random as the ambient vibration ,the dynamic properties of Yonghe Bridge is measured and the modal parameter of its 15 years after is identified and used as dynamic dactylogram of the damaged case. By the finite element analysis and locale vibration test, damaged and undamaged modal parameter is analyzed and curvature mode shape, which is sensitive to damage state, is introduced as possible candidate for identifying and locating damage in the cable-stayed bridge. Selecting the branch of part measured point from modal parameter, this paper deduces the damaged index which can detect the possible damaged location of the cable-stayed bridge deck and identifies the damaged region and degree for Yonghe Bridge. For the purpose of structural condition monitoring in in-situ , from another viewpoint, this paper introduces the method called the best optimization vector to detect damaged location and degree. The accuracy and reliability of the method presented in this paper are demonstrated by static analysis and test and the healthy state of Yonghe Bridge can be evaluated.

建立了双索面通过刚臂与主梁及边梁相连的三梁式力学模型,采用ANSYS结构分析程序对永和斜拉桥进行动力分析,对其振动模态参数进行识别,以此作为斜拉桥完好状态下的动力&指纹&;采用自由交通流作为环境振源,对永和斜拉桥动力特性进行测试,识别出永和斜拉桥使用15年后的模态参数,确立了破损状态下的动力&指纹&;利用有限元分析、现场振动测试,对破损前后的模态参数进行分析,提取对破损状况较敏感的模态曲率作为斜拉桥破损诊断的参数;并从模态参数中选取部分点的分量,导出可以指示斜拉桥桥面大概损伤位置的Index损伤指标,识别出斜拉桥发生破损的区段及破损程度;考虑大型斜拉桥在线监测要求识别破损的具体位置,从另一角度提出采用最优矢量法具体确定破损发生的位置及程度;为验证本文研究成果,对永和斜拉桥进行静力分析及静力测试,对其健康状况提出可靠的评价。

OFDA/W will normally work with the organizations on the transport issues, but Assessment Teams and DARTs will often be requested by OFDA/W to validate the field requirement for the drugs and to ensure the capability of the identified consignee to receive, transport, store, distribute, and monitor the dugs usage.

而评估小组及灾难救援应急队应灾难援助办公室要求核实对药品的需求,并确保受赠人有能力接收,运输,储藏,分发,监控药品的使用。

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On the other hand, the more important thing is because the urban housing is a kind of heterogeneity products.

另一方面,更重要的是由于城市住房是一种异质性产品。

Climate histogram is the fall that collects place measure calm value, cent serves as cross axle for a few equal interval, the area that the frequency that the value appears according to place is accumulated and becomes will be determined inside each interval, discharge the graph that rise with post, also be called histogram.

气候直方图是将所收集的降水量测定值,分为几个相等的区间作为横轴,并将各区间内所测定值依所出现的次数累积而成的面积,用柱子排起来的图形,也叫做柱状图。

You rap, you know we are not so good at rapping, huh?

你唱吧,你也知道我们并不那么擅长说唱,对吧?