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METHODS: The rib trabeculae were resected and broken, trypsinizated and washed completely by PBS. Bone surface and non-adhesive floating cells in cleaning fluid were observed with inverted microscope. Rib trabeculae was washed by DMEM culture medium once, and cultured in culture bottle. The culture liquid was replaced by new one once a week. The osteoblast was moved from the sclerite a week later. The cells were fused monolayer and could be subcultured 4 to 6 weeks later.

切下人肋骨骨小梁剪碎,胰酶消化后以PBS彻底冲洗直至于倒置显微镜下观察骨片表面及清洗液中无粘着及漂浮的细胞,以DMEM完全培养液清洗骨小梁1次,移入培养瓶内培养,每周更换培养液1次,1周后可见成骨细胞自骨片移出,4~6周细胞融合成单层可作传代培养。

Results The inpatients characterized with typical facial features, cutaneous basal cell naevus were obviously seen on the skin, palmar or plantar pits were viewed, bilamellar calcification of falx cerebri, bifid, fused or markedly splayed ribs were found, and histologically confirmed odontogenic keratocysts of jaw could be detected in most cases of BCNS.

结果 该综合征患者具有典型的面部特征,皮肤可见基底细胞痣,手掌或足底可见特殊凹陷,骨骼系统常有单侧或双侧肋骨分叉和异常钙化,大部分患者有多发性颌骨角化囊肿的口腔表现。

Considering, it is limited the cross-reactivity displayed by a single HVR peptide. We designed to ligate several high reactive HVR1 genes, and the recombinant fused protein containing several HVRls would have the ability to cross-react with most of HCV infected sera, and could be an effective immunogen of HCV vaccine.

但我们认为通过一条肽获得的交叉反应毕竟是有限的,故设想如果能将几个具有高交叉免疫反应性的HVR1片断用基因重组的方法连接起来,可能会获得与绝大多数病人血清发生免疫交叉反应性的抗原,并为疫苗的研究提供真正有效的免疫原。

The assembly of journal and plain bearing liner space is too little, force of organic still oil pressure is small it is the crucial index that affects lubricant result, make lubricate because of lacking engine oil between crankshaft and plain bearing liner and bite dead invalidation, include to be pulled badly, lack lube, layer of alloy of dry attrition generation is fused, grind sliding surface to did not maintain the oily film of certain ply, and all sorts of local overload exhaustion are destroyed, cavitation erosion and V form are destroyed etc, through technology of him director diesel locomotive old experience reachs the analysis of pair of its breakdown reasons, put forward precautionary measures especially. As a result of,the burns caustic mechanism plain bearing liner to burn caustic mechanism of 1 plain bearing liner is the lube film burst between journal and plain bearing liner, cause plain bearing liner and crankshaft generation clash, below high temperature, high pressure and high rotate speed, the wear-resisting alloy layer of plain bearing liner wears away prematurely or fuse, cause plain bearing liner and crankshaft to stick thereby bite, can make the steel back of plain bearing liner and Kong Zhong produces whirligig, inlet port is jammed, carry on the back by steel again with the friction of aperture causes high temperature to make plain bearing liner burned-out further, can cause bolt to break off, happen swing a malign accident that pounds cylinder body of bad internal-combustion engine.

轴颈和轴瓦的装配间隙太小,还有机油压力低是影响润滑效果的关键指标,使得曲轴和轴瓦之间因缺少机油润滑而咬死失效,包括严重拉伤,缺少润滑油,干摩擦产生合金层熔化,磨滑面没有保持一定厚度的油膜,以及各种局部过载疲惫破坏,气蚀和V形破坏等,通过本人主管内燃机车技术多年经验及对其故障原因的分析,特提出预防办法。1轴瓦的烧损机理轴瓦烧损的机理是由于轴颈和轴瓦之间的润滑油膜破裂,导致轴瓦和曲轴产生摩擦,在高温、高压和高转速下,轴瓦的耐磨合金层过早地磨损或熔化,从而引起轴瓦和曲轴粘咬,会使轴瓦的钢背和座孔中产生旋转运动,进油孔被堵塞,再由钢背和座孔的磨擦进一步引起高温使轴瓦烧坏,并会造成螺栓折断,发生甩出捣坏内燃机缸体的恶性事故。

Seconel to amorrhic detachment after hot press drum, not bright, fogging, self-good, non-foaming at this heat regionsun, hotmelts from the appearance to the internal deep down the entire can fully accept the role of thermal, matozoa biends a variety of raw materials in irradiated can be amply, the entire layer completely fused, creating a true, strong bond, rather than the surface, light differenc e.with shall not bend or heating occurs from carcasses, popping.

直观以复合体脱离热压滚筒后亮度高,不发雾,平度好,无起泡为基准,在这样的温度区域内,热熔胶从外表到内部深处,整个都可以充分接受热力的作用,胶体内各种原材料共混后的改性可以得到充分的发挥,整个胶层彻底熔融,产生了真正的、牢固的粘结,而非表面的、浅层次的、经不得折弯或再加热出现的离壳、起泡现象。

And with enough replications, some of these fused cells went on to form what seemed to be a very human embryo.

在复制到足够程度时,其中一些融合细胞就开始形成很像是人类胚胎的东西。

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