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Tetraploid embryos could be produced by electrofusion at the stage of two-cell embryos, which could develop to blastcysts fellowed by fusion of cytoplasm and nucleus and cleavage in vitro.After fusion of cytoplasm, the DNA methylation levels of the fused embryos was very high as well as two-cell diploid embryos in vivo.Then the embryos was rapiddly demethylated when the nucleus begin to fuse, resulting the lowest DNA methylation levels when the nucleus fused completely.After that, the DNA methylation levels of fused embryos were gradually increased until the blastocysts stage.However, whereas an asymmetric distribution of DNA methylation was established in an vivo-derived blastocysts with a higher methylation level in the inner cell mass than in the trophectoderm, in most vitro-derived tetraploid blastocysts, we can not detect the asymmetric distribution.
结果表明:利用电融合方法制备的小鼠四倍体胚胎在体外培养体系中经历细胞质融合、细胞核融合及细胞继续分裂发育直到囊胚期的过程,在细胞质融合的时候胚胎卵裂球同体内体外培养二倍体胚胎一样,呈现高度甲基化状态;在细胞核开始融合的时候,甲基化水平急速下降,在细胞核完全融合的时候甲基化水平达到最低点;随着胚胎继续分裂,胚胎甲基化水平逐渐增加,在囊胚期甲基化水平最高;但是囊胚期四倍体胚胎内细胞团同滋养层细胞甲基化荧光信号没有差别,这与体内体外培养二倍体囊胚内细胞团细胞甲基化荧光强度高于滋养层细胞甲基化荧光强度不同。
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Fuse tartar therapy:The tooth gold biotechnology product that contains a natural plant through to lift to do has no chemistry additive, just like flavor, don't need into appetite absorption, brush teeth to gargle and then can see obvious effect just after needing through contain, use in brief, safety Gao, belong to have no pain go to stone, principle be pass to refine from the plant but of essence composition, reaction happen with tartar this kind of chemistry derivatives, thus attain thorough gum fuse tartar of effect, the tooth stone calculus sheds off to recover the sheen that tooth surface originally possessed, passing to brush teeth will have been already fused of gums bottom the tartar clean up, after return can plus the 1 F protection film in the tooth surface, make the tartar no longer easily re- adhered to.
溶解牙石疗法:通过口含天然植物提练的牙齿黄金生物科技产品,无任何化学添加剂,无异味,无需进入肠胃吸收,只需要通过口含后刷牙漱口就可以看到明显效果,使用简单,安全性高,属于无痛去石,原理就是通过从植物中提炼而出的精华成份,跟牙石这种化学衍生物发生反应,从而达到深入牙龈溶解牙石的功效,牙结石脱落恢复牙齿表面原有的光泽,通过刷牙可将已溶解的龈下牙石清理出来,之后还能在牙齿表面加上一层保护膜,让牙石不再容易重新附着。
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The knife switch is also equipped with a melt fused signal indicator, when the pop-up, indicate that it has been fused melt must be promptly replaced.
在刀熔开关上还装有熔体信号指示器,当其已弹出时说明熔体已熔断,必须及时更换。
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Methods 20 human biscuspids scheduled to be extracted for the orthodontic reasons were prepared for porcelain fused to matal crown,of which every 2 teeth were from the same patient and the same jaw. The teeth were randomly, blindly and selfcontrolly divided into 2 groups.In the control group:the temporary crowns were cemented with zinc oxide cement.In the testing group:Green Or was applied before the temporary crowns were cemented with zinc oxide cement.
要领 选择因正畸治疗必要而打算撤废的20颗前磨牙举办金属烤瓷全冠牙体准备,其中每2颗牙均为同颌同名牙;授与随机、双盲、自身比较计划分为2组:比较组在牙准备体上直接用氧化锌丁喷香酚水门汀粘固且则冠;不雅察看组在牙准备体上涂布极固宁TM后用氧化锌丁喷香酚水门汀粘固且则冠。
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Methods 20 human biscuspids scheduled to be extracted for the orthodontic reasons were prepared for porcelain fused to matal crown,of which every 2 teeth were from the same patient and the same jaw. The teeth were randomly, blindly and self controlly divided into 2 groups.In the control group:the temporary crowns were cemented with zinc oxide cement.In the testing group:Green Or was applied before the temporary crowns were cemented with zinc oxide cement.
选择因正畸治疗需要而计划拔除的20颗前磨牙进行金属烤瓷全冠牙体预备,其中每2颗牙均为同颌同名牙;采用随机、双盲、自身对照设计分为2组:对照组在牙预备体上直接用氧化锌丁香酚水门汀粘固暂时冠;观察组在牙预备体上涂布极固宁TM后用氧化锌丁香酚水门汀粘固暂时冠。
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The synthesized cDNA is a continuous band less than 6. 0kb shown on x-film through α〓PdCTP which was inserted in the first strain of cDNA. The titer of the Ha-cDNA library was about 1. 5×10〓cfu/ml which could represent all protein-encoding sequences from Hz-AM1 cells; Namely, the cDNA library has representativity. After the successful construction of the Ha-cDNA library, the open-reading frame of vp39 gene of Heliothis armigera was then fused in frame with the Ga14-DNA-binding domain in the pGBT9 plasmid and the fusion protein Ga14-VP39 was expressed in yeast to be used as bait protein.
在成功构建棉铃虫细胞Hz-AM1cDNA文库的基础上,本文接着将棉铃虫核型多角体病毒衣壳蛋白VP39(HaNPV-VP39)基因克隆到酵母双杂交系统(yeasttwo-hybrid system)中的质粒pGBT9上。pGBT9上含有酵母菌转录激活因子GAL4 DNA结合域的编码区(GAL4-DBD),使克隆到质粒pGBT9上的HaNPV-vp39基因与GAL4-DBD基因融合,在酵母菌中表达DBD-vp39融合蛋白,该融合蛋白即为酵母双杂交系统中的诱饵蛋白。
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On the other hand,the shrinkage associated with the polymerization was eliminated practically by packing fine quartz sand into the fused silica tube,and the microcolumn and the wall of fused silica tube could be contacted tightly.
在C_8-硅胶整体柱制备的基础上,依靠石英砂的重力沉降,制备了直径2.0 mm,长度为10 cm的填充细石英砂的电色谱整体微柱。
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It was found that the reactions of C_(60) with anthranilic acids and isoamyl nitrite in the presence of triethylamine unexpectedly afforded C_(60)-fusedδ-lactones,which would be difficult to be prepared by other known methods.
通过在三乙胺作用下,富勒烯与邻氨基苯甲酸类化合物和亚硝酸异戊酯的环化反应,首次合成了一系列富勒烯并六元环内酯。
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Our results suggested that in lampreys and most of other vertebrates, there might be only one kininogen gene, which was fused by certain sequences during vertebrate evolution and encoded proteins with more functions; however, another special kininogen gene, only encoding the bradykinin domain with multiple copies in some species, arose only in amphibians for adapting themselves to the unique environment.
在两栖动物中,为了适应其独特的生活环境,又特化产生了另一种激肽原基因,其只编码缓激肽这一种结构域,但在某些物种中,这个结构域具有多个拷贝。
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In the present study, we collected cumulus cells oocyte complex from ovaries of two different strain mice. The cumulusenclosed oocytes were cultured for 6 h in MEM supplemented with growth factor and FSH. The meiotic maturation of these oocytes has progressed to pro-metaphse Ⅰ stage and the condensed chromosomes are visible under DIC microscope, metaphase Ⅰ spindle even can be detected under Polscope. The metaphase Ⅰ spindles of oocytes were exchanged under such microscopes. After electric stimuli, 91. 6% and 91. 6% karyoplasts-cytoplasm pairs were fused respectively. The resulting oocytes were cultured further in MEM and over 80% of oocytes released the first polar body. 79% and 77% of oocytes formed two pronuclei after in vitro fertilization and the embryos were cultured in KSOM supplemented with amino acids. Over 60% of embryos developed to blastocyst stage.
在本研究中我们在取得两种不同品系小鼠的卵丘卵母细胞复合体后,先将卵丘卵母细胞复合体置于含有多种生长因子和激素的MEM培养液中培养6小时,此时卵母细胞已进入第一次减数分裂的前中期,并且在DIC倒置显微镜下可以看到浓缩的染色体,用Polscope可以发现明显的纺锤体,借助这种显微镜通过显微操作将两种不同品系小鼠来源的卵母细胞的MI纺锤体进行互换,经过三次直流电脉冲作用后,分别有91.6%的胞质—MI核质体对融合,经过进一步的培养后,超过80%的重组卵母细胞排出第一极体,体外受精后分别有79%和77%的重组卵形成双原核,受精后的胚胎在KSOM胚胎培养液中体外培养4天后,超过60%的胚胎发育至囊胚。
- 推荐网络例句
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The split between the two groups can hardly be papered over.
这两个团体间的分歧难以掩饰。
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This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.
这种做法不仅鼓励了更多的反应,而且减少跟风的可能性。
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The new PS20 solar power tower collected sunlight through mirrors known as "heliostats" to produce steam that is converted into electricity by a turbine in Sanlucar la Mayor, Spain, Wednesday.
聚光:照片上是建在西班牙桑路卡拉马尤城的一座新型PS20塔式太阳能电站。被称为&日光反射装置&的镜子将太阳光反射到主塔,然后用聚集的热量产生蒸汽进而通过涡轮机转化为电力