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Tetraploid embryos could be produced by electrofusion at the stage of two-cell embryos, which could develop to blastocysts followed by fusion of cytoplasm and nucleus and cleavage in vitro. During the fusion of cytoplasm, the DNA methylation levels of the fused embryos are as high as these of two-cell diploid embryos in vivo Then the embryos are rapidly demethylated when the nucleus begin to fuse, resulting in the lowest DNA methylation levels when the nucleus are fused completely. After that, the DNA methylation levels of the fused embryos are gradually increased until the morula stage. However, whereas an asymmetric distribution of DNA methylation is established in vivo-derived blastocysts with a higher methylation level in the inner cell mass than that in the trophectoderm, we can not detect the asymmetric distribution in most in vitro-derived tetraploid blastocysts.

结果表明:利用电融合方法制备的小鼠四倍体胚胎在体外培养体系中经历细胞质融合、细胞核融合及细胞继续分裂发育直到囊胚期的过程,在细胞质融合的时候胚胎卵裂球同体内体外培养二倍体胚胎一样,呈现高度甲基化状态;在细胞核开始融合的时候,甲基化水平急速下降,在细胞核完全融合的时候甲基化水平达到最低点;随着胚胎继续分裂,胚胎甲基化水平逐渐增加,在桑葚胚期甲基化水平最高;但是囊胚期四倍体胚胎内细胞团同滋养层细胞甲基化荧光信号没有差别,这与体内体外培养二倍体囊胚内细胞团细胞甲基化荧光强度高于滋养层细胞甲基化荧光强度不同。

Tetraploid embryos could be produced by electrofusion at the stage of two-cell embryos, which could develop to blastocysts followed by fusion of cyto-plasm and nucleus and cleavage in vitro. During the fusion of cytoplasm, the DNA methylation levels of the fused embryos are as high as these of two-cell diploid embryos in vivo Then the embryos are rapidly demethylated when the nucleus begin to fuse, resulting in the lowest DNA methylation levels when the nucleus are fused completely. After that, the DNA methy-lation levels of the fused embryos are gradually increased until the morula stage. However, whereas an asymmetric distribu-tion of DNA methylation is established in vivo-derived blastocysts with a higher methylation level in the inner cell mass than that in the trophectoderm, we can not detect the asymmetric distribution in most in vitro-derived tetraploid blastocysts.

结果表明:利用电融合方法制备的小鼠四倍体胚胎在体外培养体系中经历细胞质融合、细胞核融合及细胞继续分裂发育直到囊胚期的过程,在细胞质融合的时候胚胎卵裂球同体内体外培养二倍体胚胎一样,呈现高度甲基化状态;在细胞核开始融合的时候,甲基化水平急速下降,在细胞核完全融合的时候甲基化水平达到最低点;随着胚胎继续分裂,胚胎甲基化水平逐渐增加,在桑葚胚期甲基化水平最高;但是囊胚期四倍体胚胎内细胞团同滋养层细胞甲基化荧光信号没有差别,这与体内体外培养二倍体囊胚内细胞团细胞甲基化荧光强度高于滋养层细胞甲基化荧光强度不同。

Fauvism can be classified as an extreme development of Van Gogh 's Post-Impressionism fused with the pointillism of Seurat [ 2 ] and other Neo-Impressionist painters, in particular Paul Signac .

野兽派可划分为一个极端的发展,凡高的后印象主义与融合在描的塞拉特 [2]和其他新印象派画家,尤其是保罗罗希涅克。

Compared with the global harmonic mapping method, this approach has the following advantages.(1) The efficiency of the algorithm is greatly improved. The computational cost involved no longer increases exponentially with the number of vertices of the meshes to be fused.(2) The algorithm is robust as the ambiguity in graph structure combination is alleviated.(3) The detail of the cut mesh is fully kept.(4) The topology restriction of the original algorithm is eliminated.

与原有的基于全局调和映射的融合方法相比,新方法的算法效率大幅度提升,求解时间不再随融合模型顶点数的增加而呈指数增长;减少了二维网格拓扑合并中奇异情况出现的概率,提高了算法的稳定性;被剪切网格的细节得到完整保留;消除了原算法对融合区域拓扑的限制。

The approach first extracts mesh regions to be fused by approximating offset curves on meshes, then parameterizes the regions using harmonic mapping with holes, and the smooth mesh fusion is finally obtained by topology combination and fusion control.

首先利用网格上的近似等距线算法来抽取出待融合区域,然后对两个待融合区域进行带内孔的调和映射参数化,最后通过拓扑合并和融合控制来实现网格的光滑融合。

RESULTS: During 10 - 12 weeks, in cuntrol group: The defect area was repaired by white and soft tissue that had no resistance to press. The repaired tissue was still lower than the surrounding articular surface with clear boundary. By histological observation, it was found that the defect was repaired by the mechanism similar to inflammatory reaction and the defect is ultimately filled by the hyperplasia of hyaline degenerative fibrous tissues. In filling group: the defect was repaired by semi-transparent, smooth, textured tissues with polish that had resistance to press as well as elasticity. The repaired tissue was almost similar to the shape of the surrounding cartilage,difficult to be distinguished. After histological observation, it was found that there was no inflammatory reaction, but active hyperplasia of inner bonetissue and cartilage tissues; a lot of osteoid tissues and trabeculation were found. Newlborn cartilage was fused with surrounding cartilage tissue and connected with surrounding tissues.

结果:10~12周,对照组:缺损区由白色、质软、按压无阻抗的组织修复,修复组织仍低于周围关节面,边界仍清晰可辨,组织学以类似炎症反应的机制修复缺损,最终以透明变性的纤维组织的增生来填补缺损部位;填充组:缺损区由半透明状、质韧光滑有光泽,按压有阻抗并有弹性的组织修复,修复组织与周围软骨外形上已基本相似,不易区分,组织学未见有炎症反应的过程,内骨组织和软骨组织增生活跃,并可见大量类骨组织和骨小梁形成,新生软骨和周围软骨组织融合,并与周围组织连接。

Even making a mouse hybridoma is tricky: B cells from the spleen of an immunized mouse, which normally died out in culture dishes, must be fused with cancerous white blood cells called myelomas.

制造小鼠的杂交瘤还有一个很难解决的问题:从免疫过的细胞脾脏中取到的B细胞,它在培养器皿中会逐渐死亡,如果一种叫骨髓瘤的白血细胞就保险得多。

The IKONOS data of Beijing Yuanmingyuan is fused with Tasseled Cap and PCA methods.The fusion results are analyzed and compared with full image and vegetation subset.The result shows that,in study area,the KT fusion method is better than PCA in full image and vegetation subset.The fusion results are differential with different KT transformation matrix,and the method of fusion should be chosen according to study area,data,and classification methods.

应用缨帽变换和主成分分析方法,对北京圆明园周围的IKONOS全色波段和多光谱波段进行融合,并分别对整景影像和植被区的融合结果进行对比研究,结果表明:在该实验区,整景影像和植被区缨帽变换融合结果都优于主成分析结果,不同的缨帽变换系数矩阵在整景影像和植被区融合质量会有差别,应根据具体地点、时相,分类方法做适当的选择。

It can be seen that the northern cloud band has already fused with the front and the convection starts to displace from the low-level circulation towards the NE.

法茜正开始转化为温带气旋,其北部云带经已跟锋面结合,而环流也被切往低层中心的东北面。

Some bone trabeculae showed medullary cavity formation. In the control sides, the grafts were fused with tibias at 4 weeks. Some iliac crest bones degraded gradually, but the bone trabeculae of grafts also could be seen in the medullary cavity after 60 weeks.

植入髂骨的对照侧4周时移植的髂骨与周围胫骨紧密愈合,部分位于髓腔内的髂骨逐渐降解,但直到60周时仍可在部分髓腔中见到移植的髂骨骨小梁。

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