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The RT-PCR product was inserted into pTG19-T vector and transformed into E. coli successfully. By blastn, the sequence results of Kunming mus musculus were in complete accordance with the conservative sequence of Genbank NR_003278 (791bp-1153bp). By Blastn in NCBI, the sequence with little difference among animals was confirmed to be conservative. After Blastn, fourteen complete CDS coding for different animals were chosen. According to VECTOR NIT 9.0 software, the similarities between Kunming mus musculus and bos taurus, homo sapiens, erinaceus europaeus, cricetulus griseus, sus scrofa, dasypus novemcinctus, rattus norvegicus, rabbit, equus caballus, macaca fascicularis, didelphis virginiana, monodelphis domestica and vombatus ursinus was 67%, 100%, 100%, 36%, 100%, 100%, 67%, 100%, 100%, 92%, 99%, 99% and 99%. In the phylogenetic tress constructed with the forteen 18S rRNA by Treeview, the Kunming mus musculus clustered with cricetulus griseus, sus scrofa and rabbit, which was nearer to cricetulus griseus and was most far away from macaca fascicularis.(3) After sencodary structure analyses of 18S rRNA of mus musculus, an oligonucleotide fragment for RNAi was designed and synthesized, which was transformed into plasmid, and restriction enzyme analyses and sequencing results should the expression plasmid pGPH1/ GFP/Neo-mouse-sh 18S rRNA were constructed for RNAi successfully.

结果①通过RT-PCR检测显示18S rRNA基因在小鼠卵巢组织和单个GV期、MⅠ期卵母细胞中均有表达，且在未成熟卵母细胞中，MⅠ期的表达明显强于GV期的表达；②RT-PCR产物克隆测序结果显示：昆明小鼠18S rRNA基因保守区序列与基因库序列[NR_003278保守区部分(791bp～1153bp)]完全一致；Blastn比对结果发现：在不同物种中差异较小，选出14种生物18S rRNA全序列经VECTOR NIT 9.0软件分析，提示昆明小鼠18SrRNA与牛、人类、刺猬、中国仓鼠、猪、犰狳、褐鼠、兔子、马、食蟹猴、负鼠、短尾猊、袋熊的18S rRNA的相似率依次为67%，100%，100%，36%，100%，100%，67%，100%，100%，92%，99%，99%，99%；Clustal 1.81和Treeview构建出的分子进化树表明：在上述14种生物中昆明小鼠与中国仓鼠进化关系最近，与兔子、猪聚成一簇，与食蟹猴进化关系最远；③根据18S rRNA二级结构设计并合成RNA干扰寡核苷酸片段，重组质粒经过限制性内切酶及测序表明成功构建了pGPH1/GFP/Neo-mouse-sh 18S rRNA干扰表达质粒。

The toxicity of spent potliner is discussed in this paper according to the research on main constitutes,microstructure,tiny section components,ash content and hydrating reactivity of SPL. The result indicates that the toxicity of SPL mostly rests with its leaching toxicity,contaminates in SPL are mainly dissolving fluorides and cyanides,these contaminates mostly come from electrolyze which infiltrate into the potliner and cause erosion,contents of dissolving F— and CN— in SPL are far more than the permitted discharge in national standard. SPL, which seriously does harm to eco-environment is classified as hazardous solid waste and should be detoxified as soon as possible.

本文从废槽内衬的物相组成、微观形貌、微区成分、灰分、水化反应性五方面研究了废槽内衬的危害性，结果表明，废槽内衬的危害性主要在于其浸出毒性，毒害物质主要是可溶氟化物和氰化物，毒害物质主要来自于电解质对内衬的渗透、侵蚀，废槽内衬中可溶F—和CN—的浓度远远高于国家标准规定的允许排放量，因此废槽内衬被认定为固体危险废弃物，严重危害生态环境，必须进行无害化处理。

The system could be used for the spectrum range from visible light (0.6328 microns) to mid-far infrared (10.6 microns), the facular size between 0.8-3 cm.

给出了测量的技术指标：光谱测量范围从可见光的0.6328微米到中远红外的10.6微米，光斑大小0.8～3cm，探测器悬臂的旋转范围±180°，样片夹持台转动范围360°，角分辨率均为0.036°。

When be being met every year to butterfly, by tens of thousands butterfly flies from far and near, flutter in fontal side all over the sky.

每年到蝴蝶会时，成千上万的蝴蝶从四面八方飞来，在泉边漫天飞舞。

Xiaoxiaomu is a working girl from yunnan who made a living in shanghai, for years, she have been looking for kangkai, when she was 14 years old, she cheated kangkai and got the imburse in order to support her younger brother to go to junior shool and university, she hope to find kangkai and apologized sincerely to him and refund the mondey so her heart can be at ease, when she saw the tired kangkai and didn't tell the truth to him, just gave up her previous work and came to kangkai restaurantto work and support this kind brother,,kangkai was dull to the feeling around him after sangjie died because he could not forget his previous wife, xiaomu feeling to kangkai is far more than thankfulness , she also found kangkai was not only strongly responsible for his marriage and family but also earnest and inflexible to his feeling , she loved him deeply.

萧晓牧是一个从云南来上海讨生活的打工妹。多年来，她一直在寻找着康凯。因为在她14岁时，她是骗得了康凯的资助，才供弟弟萧晓朴读完了高中、考上了大学。她希望能找到康凯去诚恳道歉，并还上康凯的钱以了却自己的心愿。当他看到康凯那疲惫不堪的生活状态，没敢对已经认不出自己的康凯贸然坦白，只是辞掉原来的工作，来到康凯的小餐馆里当服务员，默默地支持这个心地善良的大哥。尚洁去世后，康凯因为不能忘怀前妻，而对周围的一切感情反应麻木。晓牧对康凯的感情却不仅仅是感恩，她在和康凯的接触中，真实地感受到康凯对婚姻和家庭有着强烈的责任感，对感情认真、执著。她深深地爱上了这个大哥。

From what has been discussed above, we may safely draw the conclusion that, although extra studies inde ed enjoy many obvious advantages, its disadvantages shouldn't be ignored and far outweigh its advantages.

通过以上讨论，我们可以得出结论：尽管额外学习的确有很多优点，但它的缺点不可忽视，且远大于它的优点。

Franz Linder, president of Switzerland in particular coming from as far away as Taiwan Qingsheng chaired the press conference, and will be expressed in the Taiwan market with full confidence in the attention.

Franz林德，瑞士联邦主席，特别是来自遥远的未来，台湾庆生主持了新闻发布会，并将于在台湾市场表示了关注充满信心。

The second, so far as the text is concerned, might be considered as merely a different wording of the first, were it not that the history of the council shows that Wyclif had directly opposed the Scholastic doctrine of "accidents without a subject" as absurd and even heretical (cf, De Augustinis, De re sacramentari, Rome, 1889, II, 573 sqq.), Hence it was the intention of the council to condemn the second article, not merely as a conclusion of the first, but as a distinct and independent proposition; wherefore we may gather the Church's teaching on the subject from the contradictory proposition;"Accidentia panis manent sine subjecto," ie the accidents of bread do remain without a subject.

第二，相对於文而言，可被视为仅仅是一个不同的措辞第一，如果不是被认为安理会历史表明wyclif直接反对学术上的理论，&如果没有意外的一个主题&一样荒谬甚至异端（比照，德奥古斯蒂尼斯，德重sacramentari ，罗马， 1889年第一，二， 573 sqq ），因此这是安理会打算谴责第二篇文章，而不是仅仅作为一种结论的第一，但作为一个独特和独立命题；哪，我们可以搜集教会的教学主题，从相互矛盾的命题&； accidentia潘尼斯manent正弦subjecto &，即事故的面包做的仍然是一个课题。

The second, so far as the text is concerned, might be considered as merely a different wording of the first, were it not that the history of the council shows that Wyclif had directly opposed the Scholastic doctrine of "accidents without a subject" as absurd and even heretical (cf, De Augustinis, De re sacramentari, Rome, 1889, II, 573 sqq.), Hence it was the intention of the council to condemn the second article, not merely as a conclusion of the first, but as a distinct and independent proposition; wherefore we may gather the Church's teaching on the subject from the contradictory proposition;"Accidentia panis manent sine subjecto," ie the accidents of bread do remain without a subject.

第二，相对于文而言，可被视为仅仅是一个不同的措辞第一，如果不是被认为安理会历史表明wyclif直接反对学术上的理论，&如果没有意外的一个主题&一样荒谬甚至异端（比照，德奥古斯蒂尼斯，德重sacramentari ，罗马， 1889年第一，二， 573 sqq ），因此这是安理会打算谴责第二篇文章，而不是仅仅作为一种结论的第一，但作为一个独特和独立命题；哪，我们可以搜集教会的教学主题，从相互矛盾的命题&； accidentia潘尼斯manent正弦subjecto &，即事故的面包做的仍然是一个课题。

Marci's work had been the best course I've taken so far (and the first one from Learning Strategies I've looked into), and I'm pleased to be able to recommend it.

Marci的课程是我迄今为止看过的最好的课程(也是我浏览过的第一本学习战略的书)，我很高兴能推荐它。

I am accused of being overreligious," she said in her quiet, frank manner,"but that does not prevent me thinking the children very cruel who obstinately commit such suicide.""

客人们在卡罗利娜·埃凯家里，举止就文雅一些，因为卡罗利娜的母亲治家很严厉。

Designed by French fashion house Herm è s, this elegant uniform was manufactured in our home, Hong Kong, and was the first without a hat.

由著名品牌 Herm è s 设计，这件高贵的制服是香港本土制造，是我们第一套不配帽子的制服。