查询词典 be eventually used up

The stable clones are further identified by RT-PCR and Western blot; 6 MTT assay is used to investigate the effect of ZNRD1 on the cell growth of cells (AGS, SGC7901, MKN28, NIH3T3, GES-1); 7 Soft agar assay is used to investigate the effect of ZNRD1 on the clonality of cells (AGS, MKN28); 8 Nude mice assay is used to investigate the effect of ZNRD1 on the cell growth of gastric cancer cells (AGS, MKN28); 9 Flow cytometry is used to investigate the effect of ZNRD1 on the cell cycle distribution of cells (AGS, MKN28, NIH3T3, GES-1); 10 Flow cytometry is used to investigate the effect of ZNRD1 on the cell apoptosis of cells (AGS, MKN28, NIH3T3); 11 MTT assay is used to investigate the effect of ZNRD1 on the drug sensitivity of cancer cells (SGC7901, SGC7901/VCR, HL-60, HL-60/VCR) in vitro; 12 SRCA is used to investigate the effect of ZNRD1 on the drug sensitivity of gastric cancer cells (SGC7901, SGC7901/VCR) in vivo; 13 Flow cytometry is used to investigate the effect of ZNRD1 on adriamycin accumulation of cancer cells (SGC7901, SGC7901/VCR, HL-60, HL-60/VCR); 14 Transmission electron microscope is used to investigate the effect of ZNRD1 on the sensitivity of SGC7901 cells towards drug-induced apoptosis; 15 Flow cytometry and DNA ladder assay are used to investigate the effect of ZNRD1 on the sensitivity of cells (SGC7901, SGC7901/VCR, HL-60/VCR) towards drug-induced apoptosis; 16 Microarray is used to investigate the profiling of ZNRD1-responsive genes in gastric cancer cells (AGS, MKN28, SGC7901, SGC7901/VCR); 17 RT-PCR and Western blot are used to identify the results of microarray; 18 Reporter gene assay is used to investigate the effect of ZNRD1 on the transcriptional activity of cyclin D1; 19 Reporter gene assay is used to investigate the effect of ZNRD1 on the transcriptional activity of MDR1; 20 Kinase assay is used to investigate the effect of ZNRD1 on the activity of cyclin E-CDK2 kinase; 21 The antisensenucleic acids of p21 is used to inhibit the expression of p21, and flow cytometry is used to investigate the effect of p21 on ZNRD1-induced cell cycle arrest in gastric cancer cells; 22 The antisensenucleic acids of p27 is used to inhibit the expression of p27, and flow cytometry is used to investigate the effect of p27 on ZNRD1-induced cell cycle arrest in gastric cancer cells; 23 Liposome is used to up-regulate the expression of Skp2, and flow cytometry is used to investigate the effect of Skp2 on ZNRD1-induced cell cycle arrest in gastric cancer cells; 24 Western blot is used to investigate the effect of ZNRD1 on the stability of Skp2 and p27 in gastric cancer cells; 25 MVD assay is used to investigate the effect of ZNRD1 on the angiopoietic activity of gastric cancer cells; 26 ELISA is used to investigate the effect of ZNRD1 on the expression of VEGF165 in gastric cancer cells; 27 The roles of DARPP-32 in MDR of gastric cancer cells are investigated using gene transfection, MTT assay, SRCA, flow cytometry and DNA ladder assay.

应用杂交瘤技术制备ZNRD1的首个单克隆抗体；2）利用RT-PCR、Western blot和免疫组化检测ZNRD1在胃癌组织、胃炎组织、正常胃上皮组织、胃癌细胞和正常胃组织上皮细胞中的表达；3）构建ZNRD1的小干扰RNA载体，并测序鉴定；4）利用脂质体将ZNRD1的真核表达载体及其空载体转染胃癌细胞（AGS、SGC7901、MKN28）和小鼠成纤维细胞（NIH3T3），G418筛选后进行鉴定；5）利用脂质体将ZNRD1的小干扰RNA载体及其空载体转染药敏胃癌细胞（SGC7901）、正常胃组织上皮细胞（GES-1）、对长春新碱耐药的胃癌细胞（SGC7901/VCR）、药敏白血病细胞（HL-60）、对长春新碱耐药的白血病细胞（HL-60/VCR），G418筛选后进行鉴定；6）利用MTT实验检测ZNRD1高/低表达对细胞（AGS、SGC7901、MKN28、NIH3T3、GES-1）生长的影响；7）通过软琼脂克隆形成实验检测上调ZNRD1对AGS、MKN28细胞克隆形成能力的影响；8）通过裸鼠成瘤实验检测上调ZNRD1对AGS、MKN28细胞体内成瘤性的影响；9）通过流式细胞仪分析ZNRD1高/低表达对细胞（AGS、MKN28、NIH3T3、GES-1）的细胞周期的影响；10）通过流式细胞仪分析上调ZNRD1对细胞（AGS、MKN28、NIH3T3）的凋亡的影响；11）通过MTT实验检测ZNRD1高/低表达对细胞（SGC7901、SGC7901/VCR、HL-60、HL-60/VCR）体外药物敏感性的影响；12）通过肾包膜下移植法检测ZNRD1高/低表达对细胞（SGC7901、SGC7901/VCR）体内药物敏感性的影响；13）通过流式细胞仪分析ZNRD1高/低表达对细胞（SGC7901、SGC7901/VCR、HL-60、HL-60/VCR）内阿霉素蓄积和泵出的影响；14）通过透射电镜检测上调ZNRD1对SGC7901细胞凋亡敏感性的影响；15）通过流式细胞仪和DNA梯度试验检测ZNRD1高/低表达对细胞（SGC7901、SGC7901/VCR、HL-60）凋亡敏感性的影响；16）通过基因芯片检测ZNRD1高/低表达对胃癌细胞内基因表达谱的影响；17）利用RT-PCR、Western blot对基因芯片的结果进行鉴定；18）利用报告基因实验检测ZNRD1对cyclin D1的启动子活性的调节作用；19）利用报告基因实验检测ZNRD1高/低表达对MDR1的启动子活性的调节作用；20）利用激酶试验检测ZNRD1对cyclin E-CDK2 激酶活力的影响；21）利用反义核酸技术抑制p21的表达；通过流式细胞仪检测抑制p21对ZNRD1介导的细胞周期阻滞的影响；22）利用反义核酸技术抑制p27的表达；通过流式细胞仪检测抑制p27对ZNRD1介导的细胞周期阻滞的影响；23）利用脂质体转染法上调Skp2的表达；通过流式细胞仪检测上调Skp2对ZNRD1介导的细胞周期阻滞的影响；24）利用Western blot检测ZNRD1对p27和Skp2的蛋白稳定性的影响；25）利用微血管密度实验检测ZNRD1对AGS、MKN28细胞裸鼠移植瘤微血管形成的影响；26）利用ELISA检测ZNRD1对AGS、MKN28细胞培养上清和移植瘤匀浆中VEGF165含量的影响；27）利用脂质体转染法、MTT实验、肾包膜下移植法、流式细胞仪和DNA梯度试验检测新耐药相关分子DARPP-32对细胞（SGC7901、SGC7901/VCR、对阿霉素耐药的胃癌细胞SGC7901/ADR）多药耐药表型的影响；利用脂质体转染法和MTT实验检测下调ZNRD1对DARPP-32介导的胃癌多药耐药的调控作用。

Beyonce knowles唱的啊 You need to stop playing round with all them clowns and the wangstas Good girls gotta get down with the gangstas Go head girl put some back and some neck up on it While I stand up in the background and check up on it Ooh Boy you looking like you what you see Won't you come over check up on it,(I'm gonna let you work up on it) Ladies let em check up on it,(watch it while he check up on it) Dip it, pop it, twirk it, stop it, check on me tonight If you got it, flaunt it, boy I know you want it While I turn around you watch me check up on it Oohhh you watchin me shake it, I see it in ya face 'n' Ya can't take it, it's blazin, you watch me in amazement You can look at it, as long as you don't grab it If you don't go braggin, I'm a let you have it You think that I am teasin, but I ain't got no reason I'm sure that I can please ya, but first I gotta read ya Ooh Boy you looking like you like what you see Won't you come over check up on it (I'm gonna let you work up on it) Ladies let em check up on it (watch it while he check up on it) Dip it, pop it, twirk it, stop it, check on me tonight (x2) I can tell you wanna taste it, but I'm gone make you chase it Got to be patient, I like my men patient More patience, you take might get you in more places You can't be abrasive, has to know to pace it If I let you get up on it, you gotta make a promise That you gon put it on me, like no ones put it on me Don't bore me, just show me, all men talk but don't please I can be a tease, but I really wanna please you Ooh Boy you lookin like you like what you see Won't you come over check up on it,(I'm gonna let you work up on it) Ladies let em check up on it,(watch it while he check up on it) Dip it, pop it, twirk it, stop it, check on me tonight (x2) I'm checking on you boo, do what chu do And while dance I'ma glance at this beautiful view I'm keep my hands in my pants, I need to glue em w/ glue I'm in a trance all eyes on you and your crew Me and my mans don't dance, but to feel ya'll bump and grind If won't hurt if you gone try one time They all hot, but let me see this ones mine Its slim thug and DC outta H town Ohh Boy you looking like you like what you see Won't you come over check up on it,(I'm gonna let you work up on it) Ladies let em check up on it,(watch it while he check up on it) Dip it, pop it, twirk it, stop it, check on me tonight x4

slimthug，你必须停止玩回合与所有这些小丑与wangstas好女孩gottagetdown 随着gangstas走头女孩把一些背部和颈部一些比它在我站起背景及检查它oohboy你看你喜欢什么，你会看到你不来对清理出来，(我gonna让你工作起来就)让各位em察看着它，沾，流行资讯，twirk它，阻止它， checkonme今晚如果你有它，炫耀它，男孩我知道你要它当我转身，你看我检查它oohhh你watchin 我摇，我看它在亚面子'n'ya不能把它，它的blazin，你看我一无所有你可以看看，只要你不抓这个问题，如果你不去braggin，我是一个让你如果你觉得我teasin，但我是不会有任何理由，我相信我能请娅但是，我首先gotta阅读娅ooh男孩找你喜欢你喜欢你见到不会你来超过清理它(我gonna让你工作起来就)让各位em检查它沾，流行资讯，twirk它，阻止它， checkonmetonight(x2)，我可以告诉你想品尝，但我已离开人世，使你追逐它必须有耐心，我喜欢我的男病人更耐心，你也许可以得到你的地方越来越多，你不能磨料已经知道它的速度，如果我让你起床，那 yougotta做出承诺，你gonputitonme，不一样的说得对我并不负有我，只告诉我，所有男人说话，但不讨人喜欢，我可以戏耍，但是我真的想请你oohboylookin你喜欢你喜欢你见到不会你来对清理出来，(我gonna让你工作起来就)让各位em察看着它，沾，流行资讯，twirk它，阻止它， checkonmetonight(x2)我查你boo，做什么不楚而舞i'ma一眼这个美丽的看法，我把我的手在我的裤子，我需要胶水emw/胶水我在发呆都看你和你的船员和我我mans不跳舞，但感觉ya'll碰及研磨如果不会伤害如果你去尝试一次他们都热，但让我看到这样的矿井其slimthug直流outtah镇ohhboy你看喜欢你喜欢你见到不会你来清理，那(我gonna让你工作起来就)让各位em察看着它，沾，流行资讯，twirk它，阻止它， checkonme今晚x4

Slim Thug You need to stop playing round with all them clowns and the wangstas Good girls gotta get down with the gangstas Go head girl put some back and some neck up on it While I stand up in the background and check up on it Ooh Boy you looking like you what you see Won't you come over check up on it,(I'm gonna let you work up on it) Ladies let em check up on it,(watch it while he check up on it) Dip it, pop it, twirk it, stop it, check on me tonight If you got it, flaunt it, boy I know you want it While I turn around you watch me check up on it Oohhh you watchin me shake it, I see it in ya face 'n' Ya can't take it, it's blazin, you watch me in amazement You can look at it, as long as you don't grab it If you don't go braggin, I'm a let you have it You think that I am teasin, but I ain't got no reason I'm sure that I can please ya, but first I gotta read ya Ooh Boy you looking like you like what you see Won't you come over check up on it (I'm gonna let you work up on it) Ladies let em check up on it (watch it while he check up on it) Dip it, pop it, twirk it, stop it, check on me tonight (x2) I can tell you wanna taste it, but I'm gone make you chase it Got to be patient, I like my men patient More patience, you take might get you in more places You can't be abrasive, has to know to pace it If I let you get up on it, you gotta make a promise That you gon put it on me, like no ones put it on me Don't bore me, just show me, all men talk but don't please I can be a tease, but I really wanna please you Ooh Boy you lookin like you like what you see Won't you come over check up on it,(I'm gonna let you work up on it) Ladies let em check up on it,(watch it while he check up on it) Dip it, pop it, twirk it, stop it, check on me tonight (x2) I'm checking on you boo, do what chu do And while dance I'ma glance at this beautiful view I'm keep my hands in my pants, I need to glue em w/ glue I'm in a trance all eyes on you and your crew Me and my mans don't dance, but to feel ya'll bump and grind If won't hurt if you gone try one time They all hot, but let me see this ones mine Its slim thug and DC outta H town Ohh Boy you looking like you like what you see Won't you come over check up on it,(I'm gonna let you work up on it) Ladies let em check up on it,(watch it while he check up on it) Dip it, pop it, twirk it, stop it, check on me tonight x4

slimthug，你必须停止玩回合与所有这些小丑与wangstas好女孩gottagetdown 随着gangstas走头女孩把一些背部和颈部一些比它在我站起背景及检查它oohboy你看你喜欢什么，你会看到你不来对清理出来，(我gonna让你工作起来就)让各位em察看着它，沾，流行资讯，twirk它，阻止它， checkonme今晚如果你有它，炫耀它，男孩我知道你要它当我转身，你看我检查它oohhh你watchin 我摇，我看它在亚面子'n'ya不能把它，它的blazin，你看我一无所有你可以看看，只要你不抓这个问题，如果你不去braggin，我是一个让你如果你觉得我teasin，但我是不会有任何理由，我相信我能请娅但是，我首先gotta阅读娅ooh男孩找你喜欢你喜欢你见到不会你来超过清理它(我gonna让你工作起来就)让各位em检查它沾，流行资讯，twirk它，阻止它， checkonmetonight(x2)，我可以告诉你想品尝，但我已离开人世，使你追逐它必须有耐心，我喜欢我的男病人更耐心，你也许可以得到你的地方越来越多，你不能磨料已经知道它的速度，如果我让你起床，那 yougotta做出承诺，你gonputitonme，不一样的说得对我并不负有我，只告诉我，所有男人说话，但不讨人喜欢，我可以戏耍，但是我真的想请你oohboylookin你喜欢你喜欢你见到不会你来对清理出来，(我gonna让你工作起来就)让各位em察看着它，沾，流行资讯，twirk它，阻止它， checkonmetonight(x2)我查你boo，做什么不楚而舞i'ma一眼这个美丽的看法，我把我的手在我的裤子，我需要胶水emw/胶水我在发呆都看你和你的船员和我我mans不跳舞，但感觉ya'll碰及研磨如果不会伤害如果你去尝试一次他们都热，但让我看到这样的矿井其slimthug直流outtah镇ohhboy你看喜欢你喜欢你见到不会你来清理，那(我gonna让你工作起来就)让各位em察看着它，沾，流行资讯，twirk它，阻止它， checkonme今晚x4

I am a dream Just begin it Truth is I prayed that you open my eyes And you reveal something for me Suddenly I am no longer confined My life ain't defined by limits I Don't need no permission to live it I'm a break thru the door til I get in Everything that I got I'm a give it 100 Percent and I ain't stopping til I reach the finish I'm a believer not just a dreamer I'm givin everything I got Im a go getter 100 Percenter Undoubtedly I can reach the top And I ain't gonna let nothing discourage or dissuade me Cuz I'm walking out of here a champion either way babe I am givin it 100 Percent So go on put your ones up If you're putting in a hundred put your ones up, ones up If you know that this is something, put your ones up, ones up If you really comprehend this feel, your ones up, your ones up If you're walking into victory keep on shining One hundred percent You can only do it if you do it like I did Cause' suffering ain't easy But if you can turn your wounds into living proof That you've survived the fight and you no longer defined My life ain't defined by limits I Don't need no permission to live it I'm a break thru the door til I get in Everything that I got I'm a give it 100 Percent and I ain't stopping til I reach the finish I'm a believer not just a dreamer I'm givin everything I got I'm a go getter 100 Percenter Undoubtedly I can reach the top And I ain't gonna let nothing discourage or dissuade me Cuz I'm walking out of here a champion either way babe I am givin it 100 Percent so go on put your ones up If you're putting in a hundred put your ones up, ones up If you know that this is something, put your ones up, ones up If you really comprehend this feel, your ones up, your ones up If you're walking into victory keep on shining One hundred percent Don't you ever be discouraged, let nobody take your courage If you gonna pray don't worry, If ya gonna wait don't pray You just keep the faith and listen to me If you're putting in a hundred put your ones up, ones up If you know that this is something, put your ones up, ones up If you really comprehend this feel, your ones up, your ones up If you're walking into victory keep on shining One hundred percent I'm a believer not just a dreamer I'm givin everything I got I'm a go getter 100 Percenter Undoubtedly I can reach the top And I ain't gonna let nothing discourage or dissuade me Cuz I'm walking out of here a champion either way babe I am gotta givin 100 Percent So go on put your ones up

我是一个梦想它刚刚开始事实是我祈祷，你睁开眼睛和你透露我的东西突然，我不再局限于我的生活是没有定义的限制我不需要任何权限，它的生活我一进门新台币一中取得突破我得到的一切，我是给它百分百，我不能阻止新台币一到达终点我是一个信徒不只是一个梦我givin我得到的一切进出口一去的getter 100 Percenter 毫无疑问，我可以到达顶部我哪里也不会让任何阻止或劝阻我 Cuz我走出这里的任何一种方式宝贝冠军我givin它百分百因此，继续把你的了如果您在百把把你的了，最多的如果你知道这是什么，把你的了，最多的如果你真的理解这个感觉，你的了，你的行动如果你走进胜利继续光辉百分之百你只能这样做，如果你不喜欢我做了它事业的痛苦是不容易的但如果你能变成活生生的例子，你的伤口你已经幸存下来的斗争，你不再定义我的生活是没有定义的限制我不需要任何权限，它的生活我一进门新台币一中取得突破我得到的一切，我是给它百分百，我不能阻止新台币一到达终点我是一个信徒不只是一个梦我givin我得到的一切我是去的getter 100 Percenter 毫无疑问，我可以到达顶部我哪里也不会让任何阻止或劝阻我（没有任何东西可以阻止我） Cuz我走出这里的任何一种方式宝贝冠军我givin是百分百所以要在把你的了如果您在百把把你的了，最多的如果你知道这是什么，把你的了，最多的如果你真的理解这个感觉，你的了，你的了如果你到胜利的光辉继续行走（100）百分之百永远也不要气馁，不准任何人以自己的勇气如果您gonna祈祷不要担心，如果亚gonna等待不祈祷你只需保持这样的信念，听我说如果您在百把把你的了，最多的如果你知道这是什么，把你的了，最多的如果你真的理解这个感觉，你的了，你的了如果你走进胜利继续光辉百分之百我是一个信徒不只是一个梦我givin我得到的一切我是去的getter 100 Percenter 毫无疑问，我可以到达顶部我哪里也不会让任何阻止或劝阻我 Cuz我走出这里的任何一种方式宝贝冠军我gotta givin百分百因此，继续把你的了

The research indicated that,the 36°Bè xylose mother liquor was used as material,9.60% catalyst M-16 was added,and after caramelized 180min at 120℃,a caramel...

研究表明，在36°Be′的木糖母液中，加入9.60%的M-16催化剂，于120℃下焦糖化反应180min，得到色率为2.8万EBC单位，红色指数达6.3以上的焦糖色素。

Two merging methods of Bézier curves are generalized to tensor product Bé zier surfaces, we propose two methods to approximate two pieces of adjacent tensor product Bézier surfaces by a piece of tensor product Bézier surface. We offer the explicit formulas of the merged tensor product Bézier surfaces with all kinds of merging conditions.

同样，Bézier曲线的两种合并方法也被成功的推广到张量积Bézier曲面上，得到把两片相邻张量积Bézier曲面合并成一片张量积Bézier曲面的两种合并方法，并给出在各种合并条件下的合并曲面控制顶点的显示表示式。

Keep on struggling on Don't give up the fight We're sick and tired of your ism and skism game Die and go to heaven in Jesus' name, Lord We know when we understand Almighty God is a living man You can fool some people sometimes But you can't fool all the people all the time So now we see the light We gonna stand up for our right So you'd better get up, stand up, stand up for your right Get Up, Stand Up, don't give up the fight Get Up, Stand Up, stand up for your right Get Up, Stand Up, don't give up the fight....

继续就不要放弃斗争，我们正在生病，你的ISM和skism游戏累了模具和去到耶稣的名字天堂挣扎，当我们知道上帝全能的上帝是我们understand一个活人可以欺骗一些人You有时但你不能欺骗所有的人所有的时间，所以现在我们看到了光明我们要去站起来为我们的权利，因此，你最好站起来，站起来，捍卫你的权利站起来，站起来，不要放弃斗争起来，站起来，捍卫你的权利起床了起来，站起来，不要放弃这场斗争。。。。

Features - FSK and DTMF dual mode CLIP (Calling Line Identification Presentation)- Stores up to 50 calling numbers, up to 20 digits for each number*- Stores up to 3 last dialed numbers, up to 24 digits for each number*- Stores up to 50 PSTN Phone Book numbers, up to 12 characters for each name and 24 digits for each number (while the LCD displays the maximum of 12 digits)*- LCD displays calling and dialing number - Barring numbers setting (up to 5 numbers)*- Key lock setting - Hot call setting*- Call transfer of external calls*- Internal calls*- Three-way Conference Call*- Various ring melody (up to 9 for option)- Ring volume adjusting (up to 9 levels)- Handset earpiece volume setting (up to 9 levels)- Microphone mute - Delete single or all records in PSTN Phone Book*- Redialing and preparation dialing functions - Paging function to locate the handset easily from base station - Clicking key tone on or off - Supports GAP function - Speakerphon

特点－FSK和DTMF双模的CLIP－能存储多达50组来电，每组来电能够存储20个数字*－能够存储最近的3个已拨电话号码，每个号码能够存储24个数字*－能够存储50个PSTN电话簿号码，每个名字有12个字符，每个号码有24个数字（而LCD最多只能显示12个数字）*－LCD来电显示和去电号码－不包括号码设置（5个号码）*－键盘锁设置－热键设置*－外部来电转接*－内部呼叫*－三方电话会议*－多种铃声（一共有9种可供选择）－音量调节（一共有9个级别）－话机听筒音量设置（一共有9个级别）－静音－在PSTN电话簿内删除单个或者全部记录*－回拨和准备拨号功能－寻呼功能能够简单的从话机座定位话筒－开启或关闭滴答声－支持GAP功能－免提

Achieve a special aesthetic culture of Japan in the world, Japanese men have used aesthetic culture to control and judge class or age and social position of Japanese women, KOGAI MAGE and TAKASHIMADA MAGE is used by common women, KATSUYAMA MAGE and TENJIN MAGE is used by geshia women, HYOKO MAGE is used by concubine, RAKAKU MAGE is used by servant, SANRIN MAGE is used by young women, KUMEZO MAGE is used by a middle-aged women, MARU MAGE is used by women of old age.

日本古代发型是最足以代表日本风情的民族发式，翻开日本古代发型的演变史；最早可推到3世纪的古坟时代，历经千年以上之改良创新，而发展出多彩多姿的款式。皇族之笄髷及上流社会之高岛田髷，女医及巫女等特殊职业之片手髷，普通国民流行之桃割髷及银杏髷，艺妓专用之胜山髷及天神髷，女妾专属之兵库髷，女佣之螺角髷；少妇的三轮髷；中年之久米三髷；老年之丸髷，均有各领风骚的发型区别，毛发款式描法依照人体脸型七种样式来美化。

Nissan hopes its down-market strategy will eventually increase profits by building brand loyalty, so entry-car consumers will eventually trade up to a fatter-margin Nissan model.

按照日产的设想，先通过低端战略来建立品牌忠诚度，然后，入门车消费者最终能向高利润的日产车型升级，进而最终提升利润水平。

Listen,point and check your answers.

听，指出并且检查你的答案。

Warming needle is one of effective treatment methods for knee arthralgia aggravated by cold,and it is simple,safety,so it should be developed in clinical acupuncture and moxibustion extensively.

但以本院科针灸门诊在2005年1月—2006年6月期间共收治膝痛患者100余例，经过临床的诊断后，其中施以温针治疗的48例，疗效显著，报道如下。1临床资料本组病例48