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In this study, isobaric tags for relative and absolute quantification labeling coupled with two-dimensional liquid chromatography-tandem mass spectrometry (2D-LC-MS/MS) was employed to compare the differential protein expressions in MCF7 cells upon 17β-oestradiol (E2) stimulation or serum deprivation versus in normal conditions. A total of 576 proteins with the confidential level of ≥95% were identified, 26 proteins among the 576 proteins were found to be differentially expressed after E2 stimulation or serum deprivation, and among them 10 proteins were up-regulated and 16 proteins were down-regulated by more than two folds.

利用四重用于相对和绝对定量的等量异位标签(isobaric tags for relative and absolute quantification， iTRAQ)标记结合二维液相色谱-串联质谱(two-dimensional liquid chromatography-tandem mass spectrometry， 2D-LC-MS/MS)对雌二醇(17β-oestradiol， E2)和血清各自以及共同作用对MCF7乳腺癌细胞内蛋白质表达的影响进行了比较，共鉴定到置信度在95%以上的蛋白质576种，其中各组相对于正常培养组的细胞共找到26种差异在1倍以上的蛋白质，其中10种上调，16种下调。

Now, in a breakthrough described as the classical equivalent of finding the holy grail, Oxford University scientists have employed infra-red technology to open up the hoard, known as the Oxyrhynchus Papyri, and with it the prospect that hundreds of lost Greek comedies, tragedies and epic poems will soon be revealed.

牛津大学的科学家运用远红外技术揭开了被称为埃及奥斯莱卡浦草纸古卷的文物，这一突破性的成果被称为和发现圣杯的意义一样重大，这意味着几百部遗失的古希腊喜剧、悲剧和史诗即将重现天日。

Then peracetic acid was employed as oxidant to prepare POSS-epoxide and the epoxy value can be controlled by reaction temperature.

而后，又以过氧乙酸为氧化剂，制备了带有环氧基团的多面体低聚硅倍半氧烷分子，并可通过反应温度控制其环氧值。

In addition, the protocol is safe, convenient and proved to be widely applicable, since it was successfully employed for RNA extraction from citrus leaves, immature fruit, Poncirus seedling and citrus callus as well.

另外该方法也证明可以广泛用于柑橘叶片，未成熟的幼果，枳壳幼苗和柑橘愈伤组织的RNA提取，是一种安全、方便和适用性较广的RNA提取方法。

According to the characteristics of craftwork and micromark of quartzitic jade jues unearthed from the ancient tombs of Henglingshan site, in particular to a pair of the fine jade jues from the tomb M177, it is conjectured that the jade jues are made by the natives and the technology is employed in which fixed revolving axis in the centre when drilling a hole in jade materials, and the special fixed tools and rigid polishing materials would be used also in cutting and polishing processes.

对其中M177墓出土的一对做工精美的石英质玉玦的基本特征、工艺及微痕进行了分析，推测当时使用了可以固定旋转轴的钻孔技术、硬度很高的抛光材料或专门的&垫托&工具。

Without strong data supportance, digital agriculture cant be fully employed.

数字农业技术的实施和完善离不开各种农业资源数据的强有力支持。

Since it is the economic trade, then there may be disputes, and even the sale of even more than ordinary trouble,"meta-institutions is" contains: his wife, both into the Code-employed home and openly as husband and wife or Biqie too often there are a three-year five-year expiration date, although men say that the return of local deity, or the main tanha Code of pretty women, and then homes money, or the woman who aspire to master the economic prosperity, abandoning too fu master, a long time then fell in love, its potential may not otherwise have .

既然是经济买卖，那么就可能有纠纷，甚至比普通买卖还要麻烦，《元典章》载：其妻既入典雇之家，公然得为夫妇或婢妾，往往又有所出，三年五年期满之日，虽曰归还本主，或典主贪爱妇之姿色，再舍钱财，或妇人贪慕主之丰足，弃嫌夫主，久则相恋，其势不得不然也。

We amied the BCP sequence and the preS2 gene sequence of HBV separately as the target region, according to HBV DNA sequence of Chinese strain, synthesized the set of specific primers, amplified the sequence by PCR method from the serum of patients with CHI, verified by restriction analysis, subcloned into pGEM Teasy vectors, employed polyacrylamide gel electrophoresis to display the deletion mutation and selected the clones with differential length to be sequenced.

本研究应用PCR技术及其他分子生物学技术，分别以HBV转录调控序列BCP以及表面蛋白编码序列pre S2为靶基因，以中国株HBV基因序列为依据，设计特异性多聚酶链反应引物，分别自43例、51例慢性HBV感染患者血清中扩增出目的片段，构建质粒Teasy-BCP和Teasy-pre S2，酶切鉴定后，采用聚丙烯酰胺凝胶电泳技术展示缺失突变，再经DNA测序以了解在慢性乙型肝炎患者体内的病毒变异情况。

Due to the finer size and little dense difference between Perovskite and Titanaugite, shaking table was employed as the main equipment of gravity separation. The ground products were screened four classifications. Some qualified tailings could be cast away in the coarser fractions, which could decrease disposal quantity of the next step-floatation.

对实际改性渣进行了选择性分离，基于相对较细的粒度和二者密度相差较小的事实，选择摇床为重选设备，并采用分级入选的方式，在较粗级别可抛弃部分合格尾矿，减少磨矿和下一步重选或浮选作业的处理量。

While the problem is usually expressed as one of confidence, a more honest conclusion is that credit extended in the past has been employed unproductively and so will not be repaid according to the original terms.

虽然这个问题通常是作为一个信心，更诚实的结论是，信贷在过去一直采用和非生产性将不会归还按照原条款。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。