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By using multi-dimensional nuclear magnetic resonance method we have studied the folding mechanism of staphylococcal nuclease in vitro; the tertiary interactions for folding of SNase fragments into native-like conformation; the interaction between SNase N- and C-terminal subunits; the relationship of enzyme activity with folding and dynamic states of SNase; the structural properties of enzyme protein while exert its function. We have studied the internal motions of thermophilic Archaea protein Ssh10b and mechanism of its heat-resistance using the NMR 1H-15N relaxation and H/D exchange methods. We have determined the 3D solution structure of human translationally controlled tumor protein TCTP and the Ca2+-binding site; determined the 3D crystal structure of human mitoNEET, a novel protein from distinct groups of iron-sulfur proteins; determined the 3D solution structure of a novel chromatin protein Cren7. Determination of SNase-DNA and Archaea protein-DNA complex structures are in progress.

运用异核多维核磁共振方法研究了金黄色葡萄球菌酶体外折叠机制，酶蛋白片段体外折叠成类天然溶液三维构象的三级相互作用力，酶蛋白亚基间的相互作用，酶蛋白的折叠以及内运动状态与酶活力的关系，酶蛋白发挥功能时的结构特性；运用NMR的1H-15N 驰豫和H/D交换方法研究了嗜热古菌蛋白质Ssh10b双体结构内运动特性，热稳定性机制；确定了人翻译控制的肿瘤蛋白TCTP蛋白的溶液三维结构及其钙离子的结合部位；确定了一类新的铁硫蛋白家族蛋白人线粒体膜上mitoNEET蛋白的晶体结构；确定了一个新型的染色质蛋白Cren7的溶液三维结构；正在研究金黄色葡萄球菌酶及嗜热古菌蛋白质与DNA复合体的溶液三维结构。

Methods: the contents of amino acids are determined by hplc; tryp is determined by pico.tag; vita, vitd, vite, vitb1, vitb2, vitb6, vitb12, vpp, folic acid of formica rufa l. are determined by hplc. results: the amino acids of formica rufa l.

采用高压液相色谱仪测定蛋白质中17种氨基酸成分与含量，使用pico.tag柱前衍生法测定色氨酸成分与含量；使用高压液相色谱法测定维生素a、e、d、b1、b2、b6、b12、烟酸、叶酸等含量。

Wheat flour quality is determined by wheat quality, wheat flour milling precision is determined by process technology and equipment, and the diversification of wheat flour varieties are determined by blending system.

面粉的品质取决于原料的品质，面粉的加工精度取决于生产工艺和设备，而面粉品种的多样化取决于配粉系统。

Result: The series of Muskone can improve myocardial ischemia and infarction in experimental canines, and relieve significantly the degree of myocardial ischemia determined by epicardial electrogram mapping,decrease the range of myocardial ischemia determined by epicardial electrogram mapping and decrease infarction zone determined by N-BT staining method. And it has a significant inhibition on activity of ET induced by myocardial ischemia and infarction, and increases 6-Keto-PGF1αand 6-Keto-PGF1α/TXB2 induced by myocardial ischemia.

结果：冠心苏合丸系列组方具有明显改善犬急性心肌缺血和心肌梗死的作用，明显减轻由心外膜电图标测的心肌缺血程度及范围；减小经N-BT染色所显示的梗死区；对心肌缺血及心肌梗死引起ET的活性有明显抑制作用，同时升高血浆6-Keto-PGF1α和6-Keto-PGF1α/TXB2的比值。

Overed in the room. And through simulation test to the room in the method, the article obtains the wireless transmission model in room to carry on the confirmation to this model.Afterwards, carrying on the analysis to the edge field intensity, and determined the edgeantenna installment position. In the choice cover plan process, introduced each kind of supply oscillator use environment and determined the supply oscillator type. Explaining the distributed system, the optical fiber distribution system and so on several distributed ways. On the comparison, it had determined cover ways which the place was suitable. Through a series of improvements, the article has obtained the plan which is finally used, the antenna, the primary device, the skeleton line amplifier and so on. Afterompleting the room covers, in room distribute system will inevitably change the big net original cover situations.

通过对楼内场强进行现场DT测试，归纳出了信号在室内覆盖的薄弱环，并由此对边缘场强进行了分析，确定了边缘天线的安装位置；在选择覆盖方案的过程中，介绍了各种信号源的使用环境，针对各个场所内的电磁环境、场所的结构、楼层高度等各自不同的特点，业主对覆盖提出的不同要求，确定信号源的类型；对天馈分布系统、光纤分布系统等几种分布方式进行了比较，确定了该覆盖场所所适用的方式：通过一系列的方案组合，对几个不同的方案，根据楼域的结构以及业主的要求进行改进，归纳出了最终所采用的方案，由此确定了天线、元器件、干线放大器等的布放位置。

Crohn's disease was induced by trinitrobenzene sulfonic acid in the model group. The mice were sacrificed 5 days afterwards and LPMC were isolated and cultured. The expression of CCR2 and CCR5 on LPMC were determined by flow cytometry. The secretion of MIP-1α in the supernatant was determined by enzyme-linked immunosorbent assay, and the expression of MIP-1α mRNA was determined by reverse transcriptase polymerase chain reaction.

模型组小鼠以三硝基苯磺酸灌肠造模。5d后处死，提取和培养LPMC，采用流式细胞仪测定小鼠LPMC中趋化因子受体CCR2、CCR5的表达；采用酶联免疫吸附测定检测培养上清液中MIP-1α的分泌；采用逆转录聚合酶链反应检测MIP-1α mRNA的表达。

A method to measure the absolute velocity is developed in a spectral domain ODT system,where Doppler angle is determined by combined information on the measured Doppler shift and Doppler bandwidth,and then the flow velocity is determined from Doppler angle and Doppler shift.

发展了基于谱域ODT技术的绝对速度测量方法，该方法综合运用多普勒频移和多普勒展宽信息实现多普勒夹角的确定，进而实现流体绝对速度的测量。

K562 cell cultivated in suspension and adhesively cultivated with msc were collected respectively and cell proliferation curves were drawn; the cell cycle was determined by flow cytometry; the effect of chemotherapy on cellular viability and apoptosis of k562 cell was investigated,the mdr1 gene expression was determined by rt-pcr.

对悬浮培养和与msc黏附培养的k562细胞绘制细胞生长曲线；用流式细胞术测定细胞周期并观察化学药物对细胞存活率和凋亡的影响；用rt-pcr技术检测mdr1基因表达。

K562 cell cultivated in suspension and adhesively cultivated with MSC were collected respectively and cell proliferation curves were drawn; the cell cycle was determined by flow cytometry; the effect of chemotherapy on cellular viability and apoptosis of K562 cell was investigated, the MDR1 gene expression was determined by RT-PCR.

叶悬浮培养和与MSC黏附培养的K562细胞绘制细胞生长曲线；用流式细胞术测定细胞周期并观察化学药物对细胞存活率和凋亡的影响；用RT-PCR技术检测MDR1基因表达。

The Frechet derivative of the cost function is determined via the solution of an adjoint partial differential equation, and the boundary shape is then modified in a direction of descent. This process is repeated until an optimum solution is approached. The advantage is that the cost function variation is independent of the flow field variation, with the result that the gradient of cost function with respect to arbitrary number of design variables can be determined without the need for additional flow-field evaluations. So each design cycle requires the numerical solution of both the flow and the adjoint equations leading to a computational cost roughly equal to the cost of two flow solutions.

虽然他们的研究成果大多数都可以在公开性文献上看到，但是其中一些具体的细节问题的处理方法却没有透露，比如：伴随方程如何离散求解才能减小最终梯度计算中的误差，使得设计过程有效地进行；伴随方程与流动方程的边界条件是不同的，如何合理地处理伴随方程的边界条件，才能推动设计过程朝着设计目标而发展；在减阻问题中目标函数如何定义，求解梯度的具体数学公式如何推导，如何通过数值方法来实现它们等。

The work of this paper is as follows: 1. Looking back the progressing history of the linear motor, introducing the features of the elevator driven by linear induction motor, radicating the topic of this paper "the digital frequency variable control of the elevator bi-side direct driven by linear induction motor". The research of this paper covers the conventional VVVF control, space vector based VVVF control, vector control and DTC.

本文主要开展了以下几个方面的工作： 1 回顾了直线电机发展历史，电机的驱动技术演变，特别是针对直线电机的驱动，简要介绍了直线感应电机驱动电梯的优点和不同结构类型，对传统的v／f控制，基于空间矢量法的v／f控制，矢量控制，和基于电压空间矢量的直接转矩控制进行了比较，确立了本课题的研究主题：直线感应电机双边直推式驱动电梯的全数字变频控制。

The article combines with the treatment of a superficial civil air defense work to introduce how the grouting method to improve the performance of the backfill soil.

文章从治理漂浮人防工事的角度提出了注浆技术在改善回填土性质方面的应用，并详细阐述了注浆技术的施工流程。