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One L-isoleucine high-producing strain SW0370 was screened through protoplast fusion, shake flask compound screening, genetic marker test and single clone isolation and continuous passage cultivation.

3确定了亲株ISW330和AS1.495原生质体形成和再生及进行原生质体融合的最佳条件，采用原生质体融合技术最终筛选出一株带有目的遗传标记的L-异亮氨酸高产菌SW0370〓。

2The protoplast formation and regeneration and fusion conditions of strain ISW330 and AS 1.495 were studied in this thesis. One L-isoleucine high-producing strain SW0370 was screened through protoplast fusion, shake flask screening, genetic marker test and single clone isolation and continuous cultivation for generations.

2确定了亲株ISW330和AS1.495原生质体形成和再生及进行原生质体融合的最佳条件，采用原生质体融合技术最终筛选出一株带有目的遗传标记的L-异亮氨酸高产菌SW0370(Leu~-+Met~-+AEC~r+á-AB~r)。

One L-isoleucine high-producing strain ISW330 with genetic marker was screened through shake tube primary screening, shake flask compound screening, genetic marker test and single clone isolation and continuous passage cultivation This strain could produce L-isoleucine 9.18g/L after fermentation under the fermentation condition without being optimized.

以黄色短杆菌HL41为出发菌株，通过硫酸二乙酯和紫外线诱变处理，经摇管初筛、摇瓶复筛、遗传标记验证、单菌落分离和连续传代，筛选出一株L-异亮氨酸生产菌株ISW330(Met~-+AEC~r+α-AB~r)。

One L-glutamic acid high-producing strain TN6319 with genetic maker (FEA~r+KMA~r+SG~r ) was screened through shake tube primary screening ,shake flask second screening,genetic marker test and single clone isolation and continuous passage cultibation.this strain could produce L-glutamic 72.9g/L after fermentation 42h under the fermentation without being optimized.

经摇管初筛、摇瓶复筛、遗传标记验证、单菌落分离和连续传代，最终筛选出一株L-谷氨酸高产菌TN6319(氟乙酸~r、酮基丙二酸~r、谷氨酰胺~r、磺胺胍~r)，该菌株在未优化条件下发酵42h产L-谷氨酸79.2g/L。

The pass system and guide design key points were introduced of applying three-strand splitting rolling technology for Φ10mm ribbed bar on semi-continuous small section steel rolling line.

介绍了在集中传动的小型半连轧线上生产Φ10mm带肋钢筋采用三切分轧制工艺的孔型系统、导卫设计要点及生产中应注意的问题，现已连续生产6万余吨，成材率达101。

A reinforcement of a three-span pre-stressed continuous beam bridge is analyzed. It may provide a reference to the reinforcement of similar bridges.

3对一座三跨预应力连续梁进行了加固分析，为同类型桥梁的加固提供了参考。

2The general static performance of three-span continuous through type bow-string arch bridge is calculated.

计算得出三跨连续下承式系杆拱桥的一般受力性能。

Combined with a three-span continuous semi-supported and tied steel truss arch-bridge, structures of different joints were discussed.

结合一座正在建设的三跨连续中承式系杆钢桁拱桥的&丁&字型接头，对比了不同结构形式接头的构造。

In this paper,a research on the reinforced concrete full-scale three-span continuous slabs under fire was carried out,which underwent the normal loading.

引 言钢筋混凝土结构目前是我国建筑工程中应用最为广泛的结构形式，对钢筋混凝土结构抗火性能及灾后处理的研究是非常有实际意义的。

Based on a FE model of a three-span continuous rigid frame bridge, the updating effects of the three techniques have been evaluated when different model information, different structural parameters and different level of noise are considered.

基于一座三跨刚构－连续梁桥有限元模型，通过七种不同工况的模型修正计算，研究了这三种方法在不同模态信息量、不同误差参数以及不同噪声水平下的修正效果。

Vishnu entered a dark fourth dimensional dream that did not support his field or continued life.

毗瑟挐进入了一个第四密度的黑暗梦想，那里并不支持他的能量场或继续生命。

Leaders and decision-making persons use it to collect the data, including the information of unit work, handing in fee, oweing fee, prepaying fee,changing and afterpaying and account transfering of joining-insurance employee, and account paying of all kinds of insurances from hospitalization insurance institutions.The collected data is picked up, organized, switched and showed to user.

该子系统主要面向各级领导、决策分析人员；从各个医疗保险经办机构和定点医疗机构采集数据，包括在各个医疗保险经办机构处理的单位办公信息，单位缴费、欠费、预缴费信息，参保职工变更信息，参保职工增减变动信息，参保职工补缴信息，参保职工帐户划拨信息：包括各定点医疗机构处理的各险种帐户支出信息，各险种的统筹金支付信息等；将采集的数据提取，组织和转换，然后展示给用户。