查询词典 be at the end

We found that neither the fusioned nor the non-fusioned forms of Hu-P68 cDNA open reading frame could express efficiently in the E. coli cells; in different prokaryotic expression vectors and under various promotors resulted in no distinctive variations. Deletion of 5'UTR by synthesis adaptors, deletion of 3'UTR or signal peptide sequence by PCR gained no gross improvement. Only the truncated API-I proteins were observed to be highly expressed when the 3'ORF fragment of the Hu-P68 cDNA was fusioned at the 3'end of PAI-I gene.

结果发现无论是以融合形式存在，还是以非融合形式存在，人的P68 cDNA基因在E.coli细胞中都未能获得高效表达：在不同表达载体中，不同启动子对P68 cDNA基因的表达没有显著差异；利用合成Adapter的方法删除5'UTR，及利用PCR技术删除3'UTR和信号肽序列对表达没有明显改善；分别表达被分割的P68 cDNA5'端编码区、中央编码区和3'端编码区均未见有明显表达；当P68 3'端编码区与高效表达蛋白PAI-Ⅰ基因3'末端融合时，仅见有PAI-Ⅰ以截断形式高效表达出来。

Apartment sanitation and the use of basic configuration 两室一厅 TV seat, sofa table, Hisense TV, dining table, Galanz microwave ovens, range hood Hua Di, Haier refrigerators, Haier washing machines, Haier water heater, gas stove, two beds, two desks, drinking fountains, electric rice cooker, the United States and the fan 2 3 bed, 2 desks, sofa table, drinking fountains, tables, space refrigerator, range hood, gas stove, microwave, TV tables, combination furniture Apartment with the current system of differences Cost to pay: Water, electricity, gas and other costs from the staff since Be paid, the company conducted in accordance with the staff-level meeting Paste, the standard is: 100-level managerial / month / People; manager level and above 200 yuan / month / person 10-20 per month from the date of the General Inter-borrower to get water and electricity residential property Office documentation for reimbursement after 2009.8.1 costs generated by the former General Claims made after the documents, generated after In accordance with the provisions of the cost of the system of subsidies Apartment occupancy conditions: Yishui work in the family other than in Yishui County And higher-level managerial staff in charge of five people living in public Yu Non-managerial staff quarters to move to the company, Re-accommodation apartments on the current staff Adjustment Asset Management:- Human Resources is responsible for the apartment in the company's assets Management - At home in the music's embodied in the management of fixed assets Assets, must follow the family's possession of fixed assets Justification process, are subject to change or end-of-life through Changes in fixed assets and the company scrapped milling process procedures胡经理 Living room in 306 4 Kellogg assets All: TV 1, 2 air-conditioning, water heaters 1 to determine the disposition of assets 4

环卫公寓基本配置及使用情况两室一厅电视机座、沙发茶几、海信电视机、餐桌、格兰仕微波炉、华蒂抽油烟机、海尔冰箱、海尔洗衣机、海尔热水器、煤气灶、床2张、办公桌2张、饮水机、电饭煲、美的风扇2台床3张、办公桌2张、沙发茶几、饮水机、餐桌、航天冰箱、抽油烟机、煤气灶、微波炉、电视桌、组合家具目前公寓与制度存在的差异费用缴纳：水、电、煤气等其他费用由员工自行缴纳，公司按照员工级别进行补贴，标准为：经理级100元/月/人；经理级以上200元/月/人由总务每月10-20日期间借款，到小区物业处拿水电单据后报销 2009.8.1前产生的费用由总务取得单据后报销，以后产生的费用根据制度规定进行补贴公寓入住条件：在沂水工作，家庭在沂水县以外的经理级及以上员工有5名主管住在公寓非经理级员工搬到公司宿舍，再对目前住宿公寓的人员进行调整资产管理：-人力资源部负责公寓中公司资产的管理-在家乐氏固定资产管理中体现的资产，必须遵循家乐氏固定资产管理流程，如有变动或报废必须通过固定资产变动和报废流程手续制粉公司胡经理居住的306房中有 4项资产为家乐氏所有：电视机1台、空调2部、热水器 1台确定4项资产的处理

L Intelligent three-phase charge mode: We adopt constant current technology at the beginning of charge, which can made current invariable and avoid battery broken or accelerating battery aging. It can convert to constant- voltage and current-limiting charge when charge voltage achieves upper limit voltage, which can improve effectively the capacity conversion efficiency of battery. Trickle and floating charge make each single cell battery constant receive electricity and ensure battery capacity can be recall most, which solved the unbalanced phenomenon of single cell battery and avoid the danger of voltage change and the end of battery charge, extend battery life.

l 智能三阶段充电模式：充电初期采用恒流技术，使充电电流恒定，避免损坏电池，加速电池的老化；充电电压达到上限电压时自动转换为恒压限流充电，有效的提高了蓄电池的容量转换效率；涓流浮充使各单体电池均衡受电，保证电池容量得以最大限度恢复，有效解决单体电压不均衡现象，避免了市电电压的变化和蓄电池充电的末期造成的蓄电池过压充电的危险，大大延长了蓄电池的使用寿命。

These data suggest that the terminal hairpin structure and ITRs of PfDNV play a key role in the rescue and gene correction process: sequence at the junction of the terminal palindrome of PfDNV genome and the pUC119 can form typical Holliday structure, it can be cutted by the special endonuclease, then the exposed 3'hairpin structure serving for a self-primer to initiate viral DNA replication; and the intact extremity acting as a template to repair another altered end within inverted terminal repeats.

说明存在着一种拯救和基因修复机制，推测与PfDNV基因组末端结构（Hairpin、ITR）有密切关系：PfDNV基因组与pUC119质粒连接处可形成Holliday结构而被识别该结构的核酸内切酶所切割，游离下的基因组3'发夹结构作为引物起始复制，然后被置换链的完整的5'ITR作为模板修复部分缺失的另一端ITR，推测这个过程需要病毒NS蛋白以及细胞因子的参与。

To find an abandoned IC card, IP card, or VIP shopping malls, first spray foam on the card at one end, and then held out his hand cards will be posted to the self-adhesive foam Office, Ma Yun, one minutes later, a hand the card back and forth close to the glass blowing, the other hand then scraped off the scum, and that is very easy to put self-adhesive clear down.

找一张废弃的IC卡、IP卡或商场的贵宾卡，先将泡沫喷在卡上一端，然后用手托着卡片，将泡沫贴到不干胶处，抹匀，一分钟后，一只手用卡片紧贴玻璃来回刮，另一只手接刮掉下来的渣滓，这样很容易就把不干胶清除下来了。

You simply tell where the landscape is the beginning of the end of the building where is; Some believe that the person is the greatest destroyer of the natural ecology, history lessons too bitter Successive found a capital of Kaifeng, such as, Luoyang, Xian, the choice is, found a capital at the time, should be clean, grass long golden oriole flying beautiful places, not today picture.

你根本分不清哪里是景观的开始，哪里是建筑的结束；有的认为，人是自然生态的最大的破坏者，历史上惨痛教训不胜枚举，历代建都之地如开封、洛阳、西安等，在择都、建都之时，应是山清水秀、草长莺飞的锦绣之地，而绝非今日景象。

To further this end he brought over from England in 782 Alcuin and several of the best scholars of York, to whom he entrusted the direction of the academy established at the royal court, as well as various other schools which he caused to be started in different parts of the empire.

为了进一步为此，他带来了来自英格兰的782阿尔昆和几个最好的学者纽约，向他委托的方向学院设在宫廷，以及各种其他学校造成的，他将开始在不同的部分帝国。

For thus saith the Lord: Behold I will bring upon her as it were a river of peace, and as an overflowing torrent the glory of the Gentiles, which you shall suck; you shall be carried at the breasts, end upon the knees they shall caress you.

因为上主这样说：看！我要在她身上广赐和平，有如河流一般；我要赐给她万国的财宝，好似氾滥的江河；她的乳儿将被抱在怀中，放在膝上摇摆。

Towards the end of the 15th century, polyphonic sacred music(as exemplified in the masses of Johannes Ockeghem and Jacob Obrecht) had once again become more complex, in a manner that can perhaps be seen as correlating to the stunning detail in the painting at the time.

接近15世纪晚期的时候，复调宗教音乐（比如说Johannes Ockeghem和 Jacob Obrecht所作的弥撒曲）又再一次变得复杂，某种程度上来说有可能是因为它在当时，被看成是与表现出令人惊奇细节的绘画艺术的一种关联艺术。

Towards the end of the 15th century, polyphonic sacred music(as exemplified in the masses of Johannes Ockeghem and Jacob Obrecht) had once again become more complex, in a manner that can perhaps be seen as correlating to the stunning detail in the painting at the time.

I4 j《读者》杂志官方论坛，读者文章阅读交流——读者出版集团期刊论坛接近15世纪晚期的时候，复调宗教音乐（比如说Johannes Ockeghem和 Jacob Obrecht所作的弥撒曲）又再一次变得复杂，某种程度上来说有可能是因为它在当时，被看成是与表现出令人惊奇细节的绘画艺术的一种关联艺术。

For a big chunk of credit-card losses; the number of filings (and thus charge-off rates) would be rising again, whether

年美国个人破产法的一个改动使得破产登记急速下降，而后引起了信用卡大规模的亏损。

Eph. 4:23 And that you be renewed in the spirit of your mind

弗四23 而在你们心思的灵里得以更新