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There is neither paper about detecting DsMV by DBH or IC-RT-PCR nor report about comparing ELISA with these methods published before. Therefore, the DsMV detection methods developed in this study can be applied to virus certification scheme of calla lily and help to produce healthy seedlings in Taiwan.

之前未曾有以DBH或IC-RT-PCR检测DsMV之报告,或这些方法与ELISA比较的相关报导,因此希望本研究所研发的DsMV检测方法未来可应用於每芋种苗病毒验证作业,以协助国内业者生产健康海芋种苗。

Therefore, it is necessary to understand the relationship between carabid beetles and nvironment, and further explore the technique of carabid beetles as bioindicators, which might be applied to monitoring and evaluation of environment.

因此,深入研究步甲与环境的相互关系,在此基础上进一步研究其指示作用并应用于生态环境监测和评估之中十分重要。

The uncovered tracts and the liver wound surface were managed by some of the methods combined such as high frequency electrocoagulation, titanium clamp, suture, hemostatic gauze oppression, argon-beam coagulation, protein glue adherence, and Endo-GIA switcher. The total operative outcome, operating time, blood loss, postoperative complications and postoperative hospital stay were summarized in order to assess feasibility and safety of the clinical laparoscopic liver resection.Results: 1. Laparoscopic liver resection with clamp dissection method needs only the ordinary laparoscopic equipment. It has a low cost but causes a little more bleeding. 2. Laparoscopic liver resection operated by microwave tissue coagulation method had very little bleeding when dissecting hepatic parenchyma. The operative field is clear and the cost is low. It is a safe, convenient and effective method. 3. Ultrasound dissector is a relatively ideal appliance for laparoscopic liver resection at present because ofless bleeding during operation. But the speed is slow thus prolongs the operation time. Its separating intensity is not strong enough, and it can only be applied to patients without hepatic cirrhosis. 4. Ligsure has clamp dissection and electrocoagulation functions. It can carbonize hepatic tissue including the tracts whose diameters are less than 7mm. It has some advantage in dissecting. However, it has the shortcomings of the clamp dissector during coagulating. 5. Hand-assisted laparoscopic liver resection can make use of the flexibility and feeling of the operators left hand.

结果:1、钳夹分离切肝法不需特殊仪器设备,只要具备常规的腹腔镜器械即可实施,成本低,肝断面较易渗血;2、微波固化切肝法肝实质出血很少,视野清晰,成本低,操作简单,是一种安全、简便、有效的方法;3、超声刀是目前较为理想的切肝器械,术中出血少,但是,切割速度慢,手术时间长,而且,切割强度有限,适用于无肝硬化的肝切除;4、Ligsure具有挤压粉碎及高频电凝止血两大功能,可使包括7~以下管道的肝组织碳化、形成焦痴,具有一定的优势,但是,也存在分离钳电凝止血的缺点;5、手辅助腹腔镜肝切除可以利用术者左手的灵活性及手感,便于术中显露、分离、控制出血等,可随意协助右手及助手的主要操作,缩短了手术时间,大大提高了腹腔镜肝切除的安全性,李朝龙等的改良方法,同样达到了手辅助目的,又节省了费用;6、小切口腹腔镜辅助切肝法可以使用剖腹肝切除常规器械及剖腹肝切除技术,操作简单、可靠,适用于肝左外叶切除及右肝第V段切除;7、Endo一GIA切肝法在离断肝组织的同时闭合管道结构,多用于肝左静脉、门静脉分支、管径较大胆管的切割,缺点是不能用于较厚肝组织的切割,而且价格昂贵;8、临床巧例腹腔镜肝切除均获得成功,手术时间最短1.5h,最长sh,平均125 min,多数在100 min左右,手术出血量最少50 ml,最多500 ml,平均1 78 ml,除2例合并严重肝硬化的原发性肝癌患者术后出现少量腹水,1例术后发生胆漏外,其余无并发症,发生胆漏的1例患者术后住院40d,多数在术后一周左右出院,术后住院时间5一40d,平均gd。

Objective To discuss the question of obliguity and designate the reverse gantry CT scan and find a method which can be applied to oruebtation of centrum.

目的 探讨反角度CT扫描技术中扫描线的倾角问题及其称呼问题,确定一种能适用于各种椎体的扫描定位方法。

As cetane number of methanol is quite low, it is difficult to be applied to compression ignition engine.

根据组合燃烧发动机的特点,将发动机运行工况分为6类,针对每类工况提出不同的控制策略。

An important method of graphical model selection is to use scoring criteria to measure how well the graph structures fit the data. We present seven operators which can be applied to representations-largest chain graphs of equivalence classes.

由观察数据学习链图的结果仍然是一个等价类,当采用具有得分等价性的得分原则时,最好选择链图等价类空间作为搜索空间,这样可以避免对等价的链图重复得分。

This index has comparative characteristic that can be applied to discriminate the different city transportation performance, consequently to find disfigurements of urban transportation and help urban development.

可利用该指标对路网结构或经济结构类似的城市的交通运营状态进行比较,便于发现城市交通的缺陷,利于城市发展。

Chloride of lime at 15~30 mgL^(-1), potassium permanganate at 20~30 mgL^(-1), and 40% Yubao at 50~75μLL^(-1) coming next are recommended to be applied to the tobacco float seedling system TMY inactivation in water.

生产上池水TMV消毒可选择下列消毒剂:质量分数30%有效氯漂白粉15~30 mgL^(-1)和质量分数98%高锰酸钾20~30 mgL^(-1),其次为体积分数40% N-羟乙基亚甲胺多聚物75~100μLL^(-1)。

This principle may be applied to circular motion as well as to motion in a straight line .

这条原则不仅应用于直线运动,而且可运用于圆周运动。

It is a new monitoring and control system which could be applied to civicism construction and will bring the benefit to the city.

本系统是使用了计算机集散控制与信息管理技术的新型实用监控系统。

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But we don't care about Battlegrounds.

但我们并不在乎沙场中的显露。

Ah! don't mention it, the butcher's shop is a horror.

啊!不用提了。提到肉,真是糟透了。

Tristan, I have nowhere to send this letter and no reason to believe you wish to receive it.

Tristan ,我不知道把这信寄到哪里,也不知道你是否想收到它。