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In conclusions,(1) The pronuclear formation was asynchronous after ICSI in buffalo, the female pronuclear formed 3 hours earlier than male pronuclei;(2) After ICSI and activation, Culture of oocytes in 1.9mmol/L 6-DMAP for 3 hours can improve their subsequent embryonic development;(3) Treatment of sperm with 5mmol/L DTT can promote sperm decondensation;(4) Dead sperms can be used for ICSI in buffaloes;(5) Treatment of sperm with GSH can improve the efficiency of ICSI in buffaloes.

以上结果表明：(1)水牛精子胞质内显微受精的雌、雄原核发育不同步，雌原核比雄原核早3h形成；(2)水牛卵母细胞ICSI和激活后用1.9mmol／L 6-DMAP培养处理3h能提高其胚胎发育率；(3)DTT预处理水牛精子能提高其ICSI后的精子解聚率；(4)死精子能用于水牛卵母细胞的ICSI；(5)GSH预处理水牛精子有助于提高其ICSI后的囊胚发育率。

In the present study, we collected cumulus cells oocyte complex from ovaries of two different strain mice. The cumulusenclosed oocytes were cultured for 6 h in MEM supplemented with growth factor and FSH. The meiotic maturation of these oocytes has progressed to pro-metaphse Ⅰ stage and the condensed chromosomes are visible under DIC microscope, metaphase Ⅰ spindle even can be detected under Polscope. The metaphase Ⅰ spindles of oocytes were exchanged under such microscopes. After electric stimuli, 91. 6% and 91. 6% karyoplasts-cytoplasm pairs were fused respectively. The resulting oocytes were cultured further in MEM and over 80% of oocytes released the first polar body. 79% and 77% of oocytes formed two pronuclei after in vitro fertilization and the embryos were cultured in KSOM supplemented with amino acids. Over 60% of embryos developed to blastocyst stage.

在本研究中我们在取得两种不同品系小鼠的卵丘卵母细胞复合体后，先将卵丘卵母细胞复合体置于含有多种生长因子和激素的MEM培养液中培养6小时，此时卵母细胞已进入第一次减数分裂的前中期，并且在DIC倒置显微镜下可以看到浓缩的染色体，用Polscope可以发现明显的纺锤体，借助这种显微镜通过显微操作将两种不同品系小鼠来源的卵母细胞的MI纺锤体进行互换，经过三次直流电脉冲作用后，分别有91.6％的胞质—MI核质体对融合，经过进一步的培养后，超过80％的重组卵母细胞排出第一极体，体外受精后分别有79％和77％的重组卵形成双原核，受精后的胚胎在KSOM胚胎培养液中体外培养4天后，超过60％的胚胎发育至囊胚。

After being examined and approved by the competent departments, the application documents shall then be transferred to departments in charge of foreign trade and foreign economic cooperation of the provinces, autonomous regions and municipalities directly under the Central Government or municipalities separately listed on the State plan, after whose ratification the promotors can start to sign formal agreements and articles of association of the company.

上述文件经主管部门审查同意后，由主管部门转报省、自治区、直辖市及计划单列市对外经贸部门。上述文件经省、自治区、直辖市及计划单列市对外经贸部门核准后，发起人正式签定设立公司的协议、章程。

Driven by the police after the alarm can be sent to hospital after

后报警由警察驱赶后才得以送院治疗

However, these do not extend to the appearance of fruit after storage, and sensory evaluations will be necessary to gauge the response of panellists to factors such as the appearance of the bracts and stem, which had dehydrated noticeably after 4 weeks storage.

然而，这些并没有包括果实贮存后的外观，和评估小组成员的反应及感官评价是必要的，像是苞片和茎等外观储存4周后明显脱水。

Whenever the Carrier or Master may deem it advisable, or in any case where the goods are destined .for ports or places at which the ship does not call, and particularly but not exclusively where the-Port of Discharge from Ship and Destination of the Goods named overpage are not the same, the Carriermay, without notice, but at its own expense, forward the whole or any part of the goods before or after loading at the original port of shipment, or any other place or places even though outside the scope of the voyage or the route to or. Beyond the port of discharge or the destination of the goods bywater, by land or by air, or by any combination thereof, whether operated by the Carrier or by others and whether departing or arriving or scheduled to depart or arrive,before or after the ship expected to be used for the transportation of the goods.

十一、只要承运人或船长认为适当，或当货物被运到船只不应停靠的港口或地方，特别是当与以上规定的船舶卸货港和货物目的地不符时，承运人可不必通知，但应由自己承担费用，在装货前或后将全部或部分货物转运至原装运港口，或任何其他地方，即使不在航程内或不在通往卸货港或目的地来往航线上，用水路、陆路或航空，或其任何联运方式，不论是由承运人或其他人运作，也不论是在船被期待用于货物运输前或后离开或抵达或安排离开或抵达。

Whenever the Carrier or Master may deem it advisable, or in any case where the goods are destined for ports or places at which the ship does not call, and particularly but not exclusively where the Port of Discharge from Ship and Destination of the Goods named overpage are not the same, the Carrier may, without notice, but at its own expense, forward the whole or any part of the goods before or after loading at the original port of shipment, or any other place or places even though outside the scope of the voyage or the route to or beyond the port of discharge or the destination of the goods by water, by land or by air, or by any combination thereof, whether operated by the Carrier or by others and whether departing or arriving or scheduled to depart or arrive before or after the ship expected to be used for the transportation of the goods.

十一、只要承运人或船长认为适当，或当货物被运到船只不应停靠的港口或地方，特别是当与以上规定的船舶卸货港和货物目的地不符时，承运人可不必通知，但应由自己承担费用，在装货前或后将全部或部分货物转运至原装运港口，或任何其他地方，即使不在航程内或不在通往卸货港或目的地来往航线上，用水路、陆路或航空，或其任何联运方式，不论是由承运人或其他人运作，也不论是在船被期待用于货物运输前或后离开或抵达或安排离开或抵达。

Father Lee reveal that after super woman become all total finals, sent four words of daughter:The honor versus dishonor is not surprised, go to long sand game after, appeared not a few newses of startling statement creates a sensation on the net, her mother returned at that time afraid she can not stand, have never thought she turned over to comfort we say, if each the hearsays all wanted to care and clarify, would be very tired.

李爸爸透露，在超女成都总决赛后，送了女儿四个字：荣辱不惊，到长沙比赛后，网上出现了不少危言耸听的消息，她妈妈当时还怕她受不了，没想到她反过来安慰我们说，如果每个传言都要在意和澄清，会很累。

The strengths of the plain wood rose along with the compression ratio, while the bending strength and MOE lowered as it reached the maximum compression ratio; the plain wood compressed (33%) and heated 10h with 180℃. its bending strength lowered 40%, MOE lost about 50% and hardness lowered 12% than heated 0.5h; the properties not to be compressed after impregnated with PF resin. had a slow rising with the resin concentration, and compressed (33%) after impregnated with PF resin (30%). its bending strength, MOE and hardness increased 82.9%, 98% and 152% respectively.

三倍体毛白杨素材压缩后，其静曲强度和抗弯弹性模量均随压缩率的增大而提高，但接近最大压缩率时均下降；素材压缩（压缩率为33%）后以180℃空气加热处理10 h，静曲强度比同温度压缩保温0.5 h时下降约40%，抗弯弹性模量下降近50%，表面硬度下降约12%；用PF树脂浸渍处理后不经压缩，随PF树脂含量增加木材静曲强度提高幅度不大；用30%PF树脂浸渍后，当压缩率为33%时，其静曲强度、抗弯弹性模量和表面硬度分别比未压缩素材增加82.9%，98%和152%。

After turkey, people will use it to the trigeminal bone Wishing, usually in pairs right,trigeminal started at one end of bone fracture after breaking off a large part of the people to be - a wish.

吃完火鸡，人们还会用它的三叉骨来许愿，通常是两人一对，各执三叉骨的一端拉开，断裂后掰到较大部分的人便可许一个愿望。

The labia have now been sutured together almost completely.The drains and the Foley catheter come out at the top.

此刻阴唇已经几乎完全的缝在一起了，排除多余淤血体液的管子和Foley导管从顶端冒出来。

To get the business done, I suggest we split the difference in price.

为了做成这笔生意，我建议我们在价格上大家各让一半。