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Good retention measures after RME must be taken in order to produce adaptation in perioral soft tissues,which may contribute to stable results after orthodontic treatment.

RME治疗后必须采取良好的保持措施，使发生显著性变化的口周软组织对于扩大的牙弓发生适应性改变，这样RME治疗结果才能稳定。

After the cell growth curves was recorded, RPE cells of the 3-5th passages were utilized. 2、Three different siRNA (siRNAl,siRNA2,siRNA3) targeting against human cx43 gene and one negative control siRNA were designed and transfected into cultured human RPE cells via liposome reagent. The most effective siRNA can be determined by semi-quantitative reverse transcription PCRRT-PCR. 3、To the most effective siRNA, after transfected into human RPEs with different concentration, the cellular proliferate activities were messured by MTT colorimetry ; the percentages of RPE in different cell circle phase was assayed by FCM; the changes of phenotypical properities were observed with SCM; the protein expression of cx43 was studied through immunocytochemistry stain and Weston blot; the communication intercellular was calculated with FRAP; and the ability of recovery was assessed by using an in vitro wound healing model.4、The total proteins of siRNA1 and RPE were seperated by two-dimensional gel electrophoresis and visualized by silver staining. Proteins with significant expression alterations were selected and their peptide mass fingerprints (PMFs were obtained by matrix-assisted laser desorption/ionization time of flying mass spectrometry (MALDI-TOF-MS).The PMFs were used to search NCBInr database by Auto MS-Fit software.

实验方法：1、培养原代的人RPE细胞，经过细胞角蛋白、S-100和神经胶质原纤维酸性蛋白免疫细胞化学鉴定后，通过AO/PI染色技术确定培养细胞的存活率，描记其生长曲线，第3-5代用于以下细胞实验2、生物合成针对人cx43基因的三条小干扰RNA和一条阴性RNA通过脂质体转染RPE细胞后，通过RT-PCR的方法确定抑制效率最高的干扰片断3、将该片段以不同浓度通过阳离子脂质体转染培养的人RPE细胞后，采用MTT法观察其对细胞的增殖力的作用；通过流式细胞仪观察其对细胞周期的影响；通过扫描电镜观察其对细胞形态的影响；通过免疫细胞化学和Weston blot观察其对cx43蛋白表达的作用；采用激光共聚焦和荧光淬灭恢复技术观察荧光恢复速率平均百分率，评价其对细胞间通讯功能的影响；通过制作RPE细胞损伤模型，观察其对损伤修复能力的作用4、分离纯化转染siRNA的RPE组和正常对照组RPE细胞的全部蛋白质，应用等电聚焦电泳和SDS-PAGE双向电泳技术，银染显示分离出的蛋白质斑点，经凝胶图像分析软件对两个样本进行胶图分析，寻找差异蛋白点。

Results After onset of reperfusion, the adhesion of platelet to endothelia was observed at veins of pial vessel. they distributed as tiny spots, 30～60 minutes after reperfusion, we observed the vacuole formation in endothelial cells of artery and thrombosis could be seen at the injurious area.

结果 再灌注后即刻，软脑膜细静脉内有白细胞和血小板粘附，呈星点状分布；再灌注(30～60)min，可观察到小动脉和细动脉内皮形成的空泡和血栓形成。

Sai Di is online and brand-new measure inside the server after be being opened formally, all Sai Di is online the new personality king that the player of zone server all can play by a definite date 6 days develops class game, game time is on July 10 morning 10: 00 inside measure open to come on July 14 afternoon 17: 00 ends, after the match ends, before 4 occupational grade ranks two states 3 player can get day day, empire rich and generous platinic money large award.

赛迪在线全新服务器内测正式开放后，所有赛迪在线专区服务器的玩家均可参加为期6天的新人王冲级比赛，比赛时间为7月10日上午10：00内测开启至7月14日下午17：00结束，比赛结束后，天朝、帝国两个国家4个职业的等级排名前三名的玩家可以领取丰厚的白金币大奖。

Cotton bollwormis a polyandrous insect, and it can carry out the seconcd mating after 1d post-first-mating, and the loss substance in MAG can be compoletely supplemented with24-36hrs after mating.

棉铃虫虫是一种多次交配的昆虫，雄蛾交配后一天即可进行二次交配，交配后的棉铃虫雄蛾MAG腔内损失的物质只需24-36小时即可补充完毕。

The model of brain concussion of rat can be established successfully using iron pendulum hitting device; 2. The spatial learning and memory deficits of the rats are detected by MWM in early period post-BC(from 1 to 3 days after BC); 3. Pycnosis degeneration or necrosis neurons of the cerebral cortex, dorsal hippocampus, dentate fornix and brainstem reticular formation are identified in BC rats; 4. There are significantly changes in the number and activity of cholinergic neurons in the basal forebrain and brainstem reticular formation after BC; 5. The change of cerebral neurons and cholinergic neurons correlate with cognitive deficits in BC rats .

1、单摆式闭合性机械打击装置能成功建立脑震荡大鼠模型；2、脑震荡大鼠在MWM实验中出现早期空间认知行为障碍；3、脑震荡大鼠大脑皮质、背侧海马、齿状回和脑干网状结构出现程度不等的部分神经元固缩变性和不完全性坏死；4、脑震荡大鼠基底前脑、脑干网状结构ChAT活性表达有明显变化；5、脑震荡大鼠认知障碍与相关脑区神经元、胆碱能神经元的变化有关联。

Small Longzeyuan Changping district to be two years in the kindergarten issue has finally been resolved hope that today, Changping Commission head told reporters after the Board, property, developers, owners quadripartite discussion at the Board of Education will host three days a year without a transfer kindergarten facilities, and a week later to the tender, Please have the strength to the District Office Park kindergarten, after expert assessment and owners approved, by the end of September for the opening round.

昌平回龙观龙泽苑小区入住两年迟迟无法解决的幼儿园问题终于有了解决的希望，今天，昌平教委负责人向记者表示，经过教委、物业、开发商、业主四方讨论，教委将在三天之内接收建成一年多未移交的幼儿园设施，并在一周后向全市招标，请有实力的幼儿园入区办园，在经过专家评估和业主认可后，争取在9月底以前开园。

(1)raising of PCNA protein expression after pre-operative radiotherapy of esophageal carcinoma may signify the repopulation of tumor cells.(2) The prognosis differed from different PCNA expression before or after radiation, it may be beneficial to select the best treatment plan and as the evaluating criteria.

(1)食管癌放疗后PCNA蛋白表达升高，可能存在加速再增殖；(2)疗前、疗后PCNA蛋白表达的预测性不同，可指导临床选择最有效治疗方案，可作为评价预后指标。

After analyzing Tian and Alattar algorithm, a novel lossless information hiding method based on integer linear transform was proposed. The near character of pixels could be analyzed. After the less distorted revisable vector was transformed by integer linear transform, hidden information was embedded by pair-check modulation.

在分析Tian和Alattar隐藏算法的基础上，提出了一种基于改进整数线性变换的无损隐秘传输方法，通过分析可修改矢量区域象素的邻近相关性，选取出对图像质量影响较小的可修改矢量，进行整数线性变换后再嵌入信息。

If it is exclusive breastfeeding, nursing mothers can serve calcium, milk-fed babies have a calcium deficiency symptoms should calcium agents, such as calcium gluconate, milk half an hour after feeding, a sub-fed two or three times better absorption and also cod liver oil should continue to serve on weekdays Rideau sun 9:00 choose a more moderate after the sunlight, as much skin exposed limbs, to the best of direct sun to sun, the sun through the window glass was not effective, it should also be noted, In addition, when the sun of the need to protect your baby's eyes, not straight into the sun.

如果是纯母乳喂养，乳母可以服钙剂，奶粉喂养的宝宝有缺钙的症状就要补钙剂，比如葡萄糖酸钙，奶后半小时喂，一支分两三次喂吸收较好，另外还要继续服鱼肝油，平日里多晒太阳，选择上午9点后比较温和的阳光，尽量多露出四肢的皮肤，要直接晒到太阳最好，透过窗户玻璃的阳光没有效果，这点要注意，另外晒太阳时注意保护宝宝的眼睛，不能直对着阳光。

We have no common name for a mime of Sophron or Xenarchus and a Socratic Conversation; and we should still be without one even if the imitation in the two instances were in trimeters or elegiacs or some other kind of verse--though it is the way with people to tack on 'poet' to the name of a metre, and talk of elegiac-poets and epic-poets, thinking that they call them poets not by reason of the imitative nature of their work, but indiscriminately by reason of the metre they write in.

索夫农 、森那库斯和苏格拉底式的对话采用的模仿没有一个公共的名称；三音步诗、挽歌体或其他类型的诗的模仿也没有——人们把&诗人&这一名词和格律名称结合到一起，称之为挽歌体诗人或者史诗诗人，他们被称为诗人，似乎只是因为遵守格律写作，而非他们作品的模仿本质。

The relationship between communicative competence and grammar teaching should be that of the ends and the means.

交际能力和语法的关系应该是目标与途径的关系。