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In the model of tumor cells metastases with cell line A673, 10〓 tumor cells can be detected after only one ampliation with outer primers, which means we can find 1 tumor cells in 10〓 normal PBMC, and enlarged again with inner primers, 10〓 tumor cells can be detected, which means we can find 1 tumor cell in 10〓 normal PBMC. In the simulative model of tumor cells metastases with RNA, 10〓 tumor cell can be detected that is to say we can find 1 tumor cell in about 10〓 normal PBMC. In all 15 samples of 11 patients, 2 patients'EWS/FLI1 expressions were negative, no evidence of metastases was found followed up by 8 months and 30 months respectively. Semi-quantitative study was used to detect the expression level of EWS/FLI1 mRNA in the other 9 positive patients, and metastases occurred in 6 high-level expression patients in 2-24 months after the operation, no evidence of metastases in the other 3 low-level expression patients up to now.

应用A673细胞系建立的肿瘤转移模型，单独应用外引物进行一次扩增，可以检测到10〓级肿瘤细胞，即可以从10〓个正常细胞中检测到1个肿瘤细胞；而在此基础上再次应用内引物扩增，则可以检测到10〓级肿瘤细胞，即可以从10〓个正常细胞中检测到1个肿瘤细胞；在用RNA模拟的肿瘤细胞转移模型中，两次扩增可以检测到10〓级肿瘤细胞，即可以从10〓个正常细胞中检测到1个肿瘤细胞。11例患者的15份标本中，2例没有检测到EWS/FLI1 mRNA的表达，分别随访8个月和30个月，没有发现临床转移的证据；对其余9例阳性表达的病例进行半定量的研究，6例EWS/FLI1 mRNA高表达的病例，分别在术后2-24个月发生转移，而3例EWS/FLI1 mRNA低表达的病例，则至今没有发现转移的证据。

After analyzing and processing the grating reticle image collected by CCD, the mapping coefficient can be accurately calculated from grid size to pixels. Comparing the fore-and-aft two grating reticle images after the worktable moved. Adopting phase difference ratio arithmetic based on Lissajous figures,the phase difference of two images will be figured out. The displacement of the worktable can be further calculated by the mapping coefficient.

调整光路使CCD获取清晰的光栅图像，对图像进行分析处理，计算出栅距与像素之间的映射关系，再对工作台移动前后的光栅图像进行对比，采用基于李萨如图的相位差比率算法获取它们的像素位置差，从而计算出工作台的实际运行位移。

Alkaline lipase Greasex 50L is one of the leather enzyme products of Novo Nordisk AS. After extensive control trials on pigskins, evaluating effects by degree of fat removal and qualities of result leather, we got1 Fat removal rate can be increased by 10%and leather qualities such as napping and softness can be improved by using Greasex 50L together with liming enzyme NUE 0.6 MPX2 Some surfactant together with Greasex 50L is good to fat removal, and the recommended ratio is 0.05%～0.1%∶13 The fat content in the skins is lower after treated with Greasex 50L,which also benefits the successive processes, such as bating, pickling and tanning. As a result, the leather qualities, such as fineness of grain, softness, napping and evenness of dyeing can be improved.

Greasex 50L是诺和诺德公司生产的碱性脂肪酶已在皮革工业中广泛使用，为了更好地应用这种酶制剂，我们对Greasex 50L在猪皮方面的应用条件进行了研究采用对比的方法，用脂肪去除率和皮革的质量作为评价标准，研究结果表明1适量的浸灰酶 NUE 0.6 MPX可使Greasex 50L的脱脂率增大，同时可改善皮革的起绒状况和革身柔软性2一定量的表面活性剂与Greasex 50L配合使用，有利于脂肪的去除，用量为平平加的0.05%～0.1%3 经Greasex 50L脱脂的裸皮在鞣制后油脂含量也较低，有利于后续工序进行，从而使成革粒面平细、革身柔软，臀部起绒得到改善，染色均匀性良好

Lactating women generally do not come so early menstruation, should go to the hospital to check and remove residual intrauterine infection and organizations. 2, a long birth process, trauma, and sacroiliac joint le with relaxation, can be caused by post-natal pelvic pain, which is a normal phenomenon. 3, cesarean section should be in bed resting after a month, this period should be Shimoji activities, day 3 - 4 times; two months in bed to do gymnastics to take the initiative to help restore market size; 3 months after recovery.

哺乳期妇女一般来讲月经不会来得这么早，应去医院检查一下，排除宫内感染和组织残留。2、产程长、创伤大、骶髂关节勒带松弛，均可造成产后盆腔疼痛，这是正常现象。3、剖宫产后应该卧床静养一个月，这期间应下地活动，每天3——4次；两个月后可主动做床上体操场帮助恢复体型；3个月后基本恢复。

"Rural collective land must be converted to state-owned after the transfer to enter the secondary market" requirement, due to the former State Land Agency last century 90 In the early implementation of the collective construction of policy, this policy has a logical paradox: the state-owned land can be through the land market into the secondary market, the collective construction land must be entered into the state after the secondary market.

"农村集体土地必须转换为国有移交后进入二级市场"的要求，由于该国国土局上个世纪90年初实施集体建设的政策，这一政策有一个合乎逻辑的悖论：国有土地可以通过土地市场到二级市场，集体建设用地，必须进入状态后，二级市场。

The program is good be about to begin to come true after my website, through the effort of a week, the structure of whole website came out, repass the test of period of time, basic function was finished, in April 20 I registered a domain name.cn, but the domain name that people says Cn does not use SEO to search (those who hear, do not know to whether be true), be in then in April 24 I registered a new domain name.com again, original plan buys a good space, but still hate to part with, after all fund is limited, but I am quite lucky still, sought a cheap space, and rate is quite good, also knowing because of me the space is speed also can affect a website to rank.

规划好我的网站后就要着手去实现了，经过一个星期的努力，整个网站的结构出来了，再经过一段时间的测试，基本的功能都完成了，4月20我注册了个域名。cn，但别人说cn的域名不利用SEO搜索，于是在4月24我又注册了个新的域名。com，本来打算买个好的空间的，但还是舍不得，毕竟资金有限，但我还是挺幸运的，找了个便宜的空间，而且速度都挺不错的，因为我也知道空间是速度也是会影响网站排名的。

My baby is 5 months has not yet stand up, I also urgent, but he was 2 months when I will be when he climbed in bed, he doubled over, I do not know this has to stand up be counted, and in any case to has now been five months he has not second breakthrough, after careful observation of my husband after the baby should be like the waist is too thick, because he could have 90 degrees sideways, but did not turn over, you also have his really no way, and my friends baby 5 months when the 16 kg, has been in bed of rolling, so I think it was and build a significant relationship.

我家宝宝也是5个月还没有翻身，我也很急，不过他在2个月的时候我将他爬在床上时，他到是翻了过来，不知道这样算不算已经翻身了，反正到现在已经5个月了他还没有第二次突破，后来经过我先生的仔细观察后想宝宝应该是腰太粗，因为他早就可以90度侧身了，但是就是不翻过来，你还拿他真的没有办法，而我朋友的宝宝5个月时16斤，在床上已经滚来滚去的了，所以我想应该是和身形有相当大的关系。

I think it's a bit wrong, school, after all is for the students to learn, it should be filled with the knowledge of the breath, if the school as attractions, visitors can buy a ticket at after visiting here in take a picture and a shadow, and there, so that schools will become what ah, on the one hand, will affect students' sentiment, on the other hand, the school, the school work after be spots on the side, which is dedicated to the development of the school, or focus on how to attract more tourists to revenue?

wo觉得这样有点不妥吧，学校，毕竟是供学子们学习旳地方，它应当是充满le知识的气息，假如把学校当成景点了，游客购买了票之后就行随意进去参观，这里拍个照，那里合个影，这样学校会成啥样子啊，─方面绝对会影响学生们的情绪，另一方面，学校成为景点之后，学校工作的侧重点该放在哪呢，是专注与学校的发展，仍然专注于怎么样吸引更多的游客来创收呢？

But it only can use one time, after once puts on cannot again take off, because extinguished actually its witch collects the strength under its influence already to surmount its own limit, too crossed the exuberant flame finally to be able to burn oneself, when it is loosened once more, the technique in instantaneous will obtain certainly the big strength, but in after, also will lose his/her body for extinguished actually all abilities Constrains the word spirit: The light depends on the technique name to be able the reflective launch technique Pulls the star box: God of death aristocrat's symbol * Breaks the Asian space newly, in order to does not let the space which the present world and the corpse soul each other contact produces impractically in the traversing break the accident separately outside the samsara soul, paces back and forth is breaking, passes through year after year gathers in together, afterwards the soul can separate the memory and the energy, does for returns to the samsara to prepare.

不过它只能使用一次，一旦戴上后就不能再脱下来，因为灭却师在它的影响下其灵子收束力已经超越了其自身的极限，太过旺盛的火焰最终会烧伤自身，当它再次被解下时，术者将在瞬间得到绝大的力量，但在之后，也将失去其身为灭却师的所有能力压抑言灵：光靠术名就能反射性的发动术牵星箱：死神贵族的象征吧（朽木白哉头上的东西★新断界亚空间，为了不让现世与尸魂界彼此接触而产生的空间虚浮在穿越断界意外失散于轮回外的魂魄，徘徊在断界，经过长年累月聚集在一起，之后魂魄会分离出记忆和能量，做为重返轮回做准备

If he assumes them to be eternal and necessary, he would not admit that the First Being gives the Intelligences being after non-being, at least if "after" is taken in a temporal sense."After" could only be taken in the sense of posterior in the order of nature, according to Avicenna\'s explanation of the meaning of creation in the sixth book of his Metaphysics, chapter two.

如果他假设四因是永恒的和必须的，那至少在时间的意义上他将不会认为第一存在以非存在为基础而后派生出智慧的存在，根据阿维森纳在其《形而上学》第二章第六部分中对创造的阐释，&而后&只能是自然秩序的后天的意义。

Singer Leona Lewis and former Led Zeppelin guitarist Jimmy Page emerged as the bus transformed into a grass-covered carnival float, and the pair combined for a rendition of "Whole Lotta Love".

歌手leona刘易斯和前率领的飞艇的吉他手吉米页出现巴士转化为基层所涵盖的嘉年华花车，和一双合并为一移交&整个lotta爱&。

This is Kate, and that's Erin.

这是凯特，那个是爱朗。