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In this article, we studied some components in giant panda seminal plasma and their relationship with semen characteristics.The protein bands were separated by SDS-PAGE, platelet-activating factor acetylhydrolase activity and glutamic-aspartic aminotransferase activity were detected by ELASE and chymic colorimetry respectively. The results are as followed:Twelve protein bands in giant panda seminal plasma were identified by SDS-PAGE, the molecular weights were 74.9、59.6、 56.2、 54.7、 47.3、 38.2、 30.8、 23.8、 18.4、 16.8、14.0 and 13.4kDa respectively, six of them (74.9, 59.6, 56.2, 47.3, 30.8 and 13.4kDa) werepresent in all samples. However, there was no relationship was found between the abnormalprotein bands appeared in several samples and semen characteristics in our experiment.

本研究采用SDS-PAGE对大熊猫精浆蛋白电泳谱带特性进行分析,使用酶联免疫方法检测精浆血小板激活因子乙酰水解酶活性、化学比色法检测精浆谷草转氨酶活性,并初步探讨了这些物质与大熊猫精液质量的关系,结果显示:(1)大熊猫精浆SDS-PAGE电泳共分离得到12条蛋白谱带,其分子量分别为74.9kDa、59.6kDa、56.2kDa、54.7kDa、47.3kDa、38.2kDa、30.8kDa、23.8kDa、18.4kDa、16.8kDa、14.0kDa和13.4kDa,其中74.9kDa、59.6kDa、56.2kDa、47.3kDa、30.8kDa、13.4kDa谱带为所有个体大熊猫精浆样品所共有。

Typical bands were used to analyze relation with ecotypes and characters of fruits, indicating that there was no relation between typical bands and ecotypes, but some extent relation between bands and characters of fruits existed. If all cultivars were divided into freestone and clingstone, melting and nonmelting, both freestone (ratio shared total survey, 41/56=73.2%) and clingstone (ratio shared total survey, 33/79=41.8%) appeared in the loci of S_(167)-1050 bp linked marker raised by Warburton et al. It was apparently not strong linkage between the marker and the character, and exchange rate of genes was high. If combinations of S_(167)-850bp and S_(167)-1050bp or S_(167)-850bp and S_(167)-1400bp were used to distinguish characters of fruits, 6 freestone accessions without clingstone ones and 6 melting accessions without nonmelting ones displayed in the first combination of markers, 20 clingstone accessions and 2 semi-freestone ones without freestone ones appeared in the second combination of markers.

通过典型带对不同生态型、果实性状为类别进行分析,结果未发现有与生态型相吻合的特征带出现,而将供试所有品种按离核、粘核和溶质、不溶质划分归类,供试品种在Warburton等得到S_(167)-1050 bp处粘离核分子标记,出现既有离核(占供试离核41/56=73.2%),又有粘核(占供试粘核33/79=41.8%),可见此连锁标记连锁性不强,发生交换的机率较高,而S_(167)-850bp带和S_(167)-1050bp带同时出现时,该组包含6个离核,无粘核品种;6个溶质,而无不溶质。S_(167)-850bp和S_(167)-1400bp同时出现时,包含20个粘核品种和2个半离核品种。

FT-IR spectrum showed that the new bands at 3000cm-1~2700cm-1 and 1600cm-1~1400cm-1 were assigned to the C-H telescopic vibration and the framework vibration feature of imidazole ring, besides the characteristic bands of BMMs appeared in all samples, however, all of characteristic bands for imidazolium ionic liquid disappeared in the samples when calcined at 550oC, which implied that the structure of imidazolium ionic liquid has been destroyed during calcinations.

FT-IR分析测试中3000cm-1~2700cm-1及1600cm-1~1400cm-1波数范围内的谱带被认为是饱和的C-H伸缩振动和芳环骨架振动,说明离子液体的存在,这些峰只在萃取样品中出现,可见键连的离子液体的结构在焙烧过程中被破坏。

Twenty-four random primers were used to analyze the genomes of three descendant strains with steady hereditable variation produced form domesticated silkworm by artificial insemination with sperms of eri silkworm. The results show that in the RAPD patterns there are many amplified bands called" parental bands"which are similar to those of the female parent. At the same time, there appears varied amount of amplified bands called "variant bands" that are different from those of the female parent. The variant ...

采用24种随机引物,对以蓖麻蚕精子进行人工授精得到的家蚕后代中的3个稳定变异品系及其亲本的基因组进行了RAPD检测,结果显示,在变异品系的RAPD图谱中,不仅存在大量与母本相同的&亲本带&,同时还出现了不同数量与母本不同的&变异带&,包括&非亲本带&、&缺失带&及个别仅与父本相同的&目的带&,从分子水平上揭示了变异品系存在着明显的&偏母性&与&变异性&特点。

These primer combinations produced a total of 1 119 bands, 229 specific loci (including 54 absent bands, and 175 monomorphic bands). Among them, 983 polymorphic bands, accounting for 88%, were detected. The percentage of identification per primer combination was as high as 100%. The average PPB of 14 foreign cultivars was 35.86% and the average PPB of seven domestic cultivars was 31.51%.

结果表明:8对引物共产生1 117条谱带, 229个特异位点(其中缺失带54条、单态带175条),多态带983条,多态带的比例为88%;每对引物鉴别效率为100%。14个国外银杏观赏品种平均多态带的比例35.86%,7个国内品种平均多态带的比例31.51%。21个观赏品种之间相似系数为0.4899~0.8499。

Five polymorphic primers with good, clear bands were got, screening fromseventy-five ISSR primers. These five pairs of primers were used to amplificaterespectively the genomic DNA of 66 different Pterocarya stenoptera individuals and atotal of 45 bands were amplified with 43 polymorphic bands and percentage ofpolymorphic bands 95.6%.

2从75条ISSR引物中筛选出5条多态性好、谱带清晰的引物,用这5条引物对66个枫杨叶样的基因组DNA进行了PCR扩增,共得到45条带,其中多态性带43条,多态性百分率为95.6%。

Moreover, it added two bands of higher molecular weight and one band which molecular weight is about 25KD, but lost two bands of smaller molecular weight. The bands of peroxidase isozymes showed vitrous plantlets lost three bands such as Rf0.245, Rf0.309, Rf0.382; but added one band of Rf0.200, and the activity of Rf0.136 was increased.

过氧化物酶电泳表明,玻璃苗缺失了Rf值分别为0.245、0.309和0.382的三条谱带,同时增加了Rf值为0.200一条谱带,而Rf值为0.136的谱带活性要明显高于正常苗。

Number of polymorphic bands changed after irradiation. Flawing bands and increasing bands were observed in amplified bands of 14 selected ISSR primers.

双环蘑菇变异最大,200和500Gy辐照引起的ISSR变异率分别为33.3%和50.5%;金针菇变异最小,200、500和800Gy下的ISSR变异率分别为2.3%、2.3%和3.1%,杏鲍菇菌丝的变异率分别为8.6%、13.4%和20.0%。

Three factors, the information amount of bands, correlation between bands and separability of objects in bands, are considered in selecting bands. The major steps of band selection are

与常规遥感数据不同,从高光谱数据选出的最佳波段要多得多,而且它们之间往往存在相关性,因此在波段选出后,还应对它们进行特征提取。

There are the common specific bands on the zymogram of three isozymesfrom the lamina for every population of Pinellia ternata, e.i. two bands of EST,three bands of SOD and one bands of POD.

同工酶分析结果表明,三种同工酶酶谱在各居群间均有共同特征谱带,其中EST同工酶有2条,SOD有3条,POD有1条。

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