查询词典 bacterial protein

SDS-PAGE analysis revealed that the GST-HF93 fusion protein had an apparent molecular weight of 38 kD and accumulated up to 50% of bacterial soluble proteins.

表达产物经谷胱甘肽S转移酶亲和层析柱纯化，得到的融合蛋白纯度达90％以上。

The results indicatethat the expression of Cyt1A alone could result in the cytolysis of host bacterial cells,and both P20 and Cry11A could prevent bacterial cells from cytolysing caused byCyt1A protein.

为了进一步探讨P19和P20对Cry11A和Cyt1A相互作用的影响，本研究构建了表达P19、P20、Cry11A和Cyt1A不同组合的七个Bt重组菌株，分别从目的蛋白表达、营养细胞生长、芽胞和晶体形成、杀虫毒力等方面进行了研究。

Tomatoes Tomato fruit contains is very rich in protein, fattiness, carbohydrate, line acid, carotene, vitamin B1, B2, C, etc, it also contains promoting growth of the teenagers of calcium, phosphorus, iron and other minerals, and inhibiting bacterial growth of tomato, tomato.

番茄 番茄果实含有十分丰富的蛋白质、脂肪、碳水化合物、炯酸、胡萝卜素、维生素B1、B2、C等，它还含有促进青少年生长发育的钙、磷、铁等矿物质，以及抑制细菌生长的番茄素。

Bacterial flagella are thread-like appendages composed entirely of protein.

完全由蛋白质构成的一种丝状的附属结构。

Therefore, CRP could mediate E-LDL participating in the early stage of atherogenesis. CRP is an ancient conserved protein whose initial role as a pattern recognition molecule may have been to defend against bacterial infections, but whose present biological role appears quite complex. CRP is protective against a variety of bacterial infections and inflammatory stimuli as an opsonin. It is likely that the activity of CRP in humans is either pro- or anti-inflammatory is dependent on the context in which it is acting.

由于CRP的识别相可以结合被暴露出磷酸胆碱结构的E-LDL，形成E-LDL／CRP复合体，而内皮细胞表面能以高亲和力识别CRP的配体相，从而CRP-相性结构具有结合E-LDL，被内皮细胞捕获，引起脂质沉积和激活补体、触发炎症反应的可能性，这样可以解释早期动脉粥样硬化为何同时存在E-LDL、CRP、补体，而这三种物质在同时、同地出现绝非一个巧合。

The present invention relates to a process of producing a functional immunoglobulin superfamily protein, which has at least one disulphide bond when functional, the process comprising the steps of providing a bacterial cell comprising a gene coding for the protein, the gene is expressible in said cell, cultivating the cell under conditions where the gene is expressed, isolating the protein from the cell without reducing it, and subjecting the isolated protein to a folding treatment.

本发明涉及一种产生功能性免疫球蛋白超家族蛋白的方法，此蛋白质在有功能时具有至少一个二硫键，此方法包括如下步骤：提供一种包含编码此蛋白质的基因且此基因可在其中表达的细菌细胞；在使此基因表达的条件下培养此细胞；从该细胞中分离出此蛋白质而不还原它；以及使分离出的蛋白质经受折叠处理。

The N gene ORF was then subcloned into pET-30a vector and the recombinant plasmid was transformed into E.coli BL21 (DE3) and induced with IPTG. The protein expression was determined by SDS-PAGE. The expressed protein had a molecular weight of 54.4 Ku that existed as inclusion body. Thin-layer scanning showed that the expression product accounted for 30.5% of the total bacterial proteins. The recombinant protein possessed native biological activity and could react with anti-PEDV hyperimmune serum in Western blot.

以阳性质粒为模板，用分别含有BamH Ⅰ和Xho Ⅰ酶切位点的上、下游引物扩增得到ORF，其PCR产物经BamH Ⅰ和Xho Ⅰ双酶切后定向克隆到pET-30a载体，构建的重组质粒命名为pET-30a-PN；将pET-30a-PN转化到大肠杆菌BL21 (DF3)中，在IPTG诱导下进行表达；SDS-PAGE结果表明表达出与预期大小相符的约54.4 Ku的重组蛋白，重组蛋白以包涵体形式存在；薄层扫描结果表明表达产物占菌体总蛋白的30.5%；Western blot分析表明表达的重组蛋白能与抗PEDV高免血清反应，说明该重组蛋白具有免疫学活性。

①The collected clinical Acinetobacter baumannii isolates were more seriously resistable to Ciprofloxacin than to Imipenem;②The antibiotic resistance of clinical Acinetobacter baumannii isolates may be associated with the decline of CarO protein expression;③There is some relationship between CarO protein and ciprofloxacin-resistant Acinetobacter baumannii strains,it proves that CarO protein supports bacteria on multi-drug resistance;④CarO protein may be related to cell signalling;⑤The difference in CarO protein functional sites and structure between the sensitive strains and the resistant strains may be related to bacterial resistance.

①收集的临床鲍曼不动杆菌对环丙沙星的耐药情况较亚胺培南严重；②CarO蛋白的表达下调可能与临床鲍曼不动杆菌的耐药性相关；③CarO蛋白与鲍曼不动杆菌耐环丙沙星有一定相关性，佐证了CarO蛋白可能与细菌多重耐药有关；④CarO蛋白可能与细胞信号传导有关；⑤敏感菌株与耐药菌株的CarO蛋白功能位点和内部结构有差异，可能与细菌耐药有关。

For further understanding the effect of the strain on K-bearing silicate mineral, an experiment with KO2 strain cultured in nitrogen-containing and nitrogen-free medium was conducted. By putting mineral powders into the medium and measuring the concentration of capsular polysaccharides, we concluded that the strain can accelerate the weathering rate of silicate minerals. The results showed that the polysaccharides secreted by the strain in the growth process could help the bacterial to adhere to the mineral surface effectively, and created the bacterium-mineral complex, which formed a mircro-enviorment avail the release of potassium. Another result was that there was higher level of Carbonic Anhydrase activity, which revealed that some exo-protein or enzyme produced by the bacterial had postive impact on the process of releasing potassium ion. We carried out the bacterial fermentation for larger scale production of the bacterial secretion, which was used to sperate, and identify the small molecules related to the mineral-bacterial interaction. After the analysis, it was found that the strain can produce many kinds of small molecules, such as 2-Hydroxybenzoic acid, 4-Hydroxyphenylacetic acid, methyl-4-hydroxybenzoate, etc.

分别将菌株接入含有不同矿粉的培养基中培养，检测其在有氮、无氮培养基中荚膜多糖含量的变化，并在以钾长石和黑云母为矿源的情况下，比较研究了菌株在有氮、无氮培养基中对矿物的风化能力，通过一系列的实验，证实胶质芽孢杆菌在生长过程中所分泌的粘稠胞外聚糖可帮助细菌有效黏附在矿物表面，形成细菌-矿物复合体，并改变及维持该复合体内部的微环境，有助于该菌的解钾作用，而且细菌分泌的胞外蛋白质在该菌对含钾硅酸盐矿物的解钾作用过程中发挥重要作用；将胶质芽孢杆菌接入以钾长石和黑云母为矿源的有氮、无氮培养基中培养，检测到该菌碳酸酐酶活性的变化，并进行批量发酵后小分子酸性分泌产物的提取、分离、纯化与鉴定等方面的研究，结果表明，该菌可产生2-羟基苯甲酸，4-羟基苯乙酸，4-羟基苯甲酸甲酯等小分子物质。

Zuo Tian M80 with beneficial bacteria and bacterial protein organic raw materials, through the Japan's Central Engineering Co.,Ltd.Open aerobic fermentation system,using modern microbial fermentation technology, the depth of maturity by the production of Zoeticland card with fertilizer,was awarded for its excellent quality a number of appraisal,has won:China International Vegetable Science and Technology Fair gold medal, standardized production to promote their products and vegetables in Shouguang,Shandong Nongji Xie focusing on promoting technology projects,Jinan Municipal Bureau of Agriculture Farmers trusted quality products.

采用佐田氏M80有益菌和菌体蛋白有机原料、通过日本日环工程株式会社开放式有氧发酵系统，运用现代微生物发酵技术，经深度腐熟生产的&佐田氏&牌有肥料，以其优良的品质荣获了多项成果鉴定，先后获得：中国国际蔬菜科技博览会金奖、寿光蔬菜标准化生产推广产品、山东农技协重点推广技术项目、济南市农业局农民信得过优质产品。

According to the clear water experiment, aeration performance of the new equipment is good with high total oxygen transfer coefficient and oxygen utilization ratio.

曝气设备的动力效率在叶轮转速为120rpm～150rpm时取得最大值，此时氧利用率和充氧能力也具有较高值。

The environmental stability of that world - including its crushing pressures and icy darkness - means that some of its most famous inhabitants have survived for eons as evolutionary throwbacks, their bodies undergoing little change.

稳定的海底环境─包括能把人压扁的压力和冰冷的黑暗─意谓海底某些最知名的栖居生物已以演化返祖的样态活了万世，形体几无变化。