查询词典 bacillus

in order to establish a rapid, sensitive and specific detection method for identification of four kinds of food-borne pathogenic bacteria including salmonellla spp, listeria monocytogenes, staphylococcus aureus and bacillus cereus by using multiplex pcr, according to invasion protein a geneof salmonellla spp, transcriptional regulatory protein gene of listeria monocytogenes, autolysin gene ofstaphylococcus aureus and gyrase b subunit gene of bacillus cereus, four pairs of specific primers were designed for multiplex pcr amplification.

小 摘要：为建立一种利用多重pcr技术检测和鉴定沙门氏菌、单核细胞增生李斯特菌、金黄色葡萄球菌和蜡样芽孢杆菌的方法，根据沙门氏菌侵袭力蛋白a基因、单核细胞增生李斯特菌蛋白转录调控基因、金黄色葡萄球菌自溶素基因、蜡样芽孢杆菌的促旋酶b亚单位基因设计引物，进行多重pcr扩增，并对多重pcr反应体系进行优化。

This organization of pro A and proE from Bacillus subtilis 93151 was first reported in Bacillus subtilis.

这是在枯草芽孢杆菌中首次发现的proB和proA基因重叠的组织方式。

Resulls showed that five stored antagonists (Burkholder cepacia, Candida guiliermondii, Kloeckera apiculata, Debaryomyces han-senii, Bacillus subtilis) could effectively inhibit brown rot of sweet cherry fruit, among which Bacillus subtilis and Candida guiliermondii could entirely inhibit the disease.

结果表明，保存的洋葱伯克霍尔德氏菌、季也蒙假丝酵母、柠檬形克勒克酵母、汉逊德巴利酵母、枯草芽孢杆菌 B-912等5种拮抗菌对甜樱桃褐腐病都表现出显著的抑制效果，其中枯草芽孢杆菌、季也蒙假丝酵母可完全抑制病害的发生；季也蒙假丝酵母和柠檬形克勒克酵母能有效地防治核果类果实采摘后软腐病。

The identification on the physiological and biochemical characteristics of F1-5 showed that it was Bacillus subtilis or Bacillus cereus.

对F1-5进行生理生化特征鉴定，结果表明，其为枯草芽孢杆菌或蜡样芽孢杆菌。

From 1983 since, american researcher already produced toxin of an anthrax bacili, A flesh successfully with contemporary biology technology family name of congratulate of bacillus, choleraic vibrio, annals is dysenteric the gene of bacillus and toxin of buccal larynx bacili.

从1983年以来，美国探究人员已用现代生物技术成功生产出炭疽杆菌、A 型肉毒杆菌、霍乱弧菌、志贺氏痢疾杆菌和口喉杆菌毒素的基因。

After washing, 5 ml of the blood was taken to culture, and 200～300 ml of the blood was taken to add with staphylococcus aureus, bacillus pyocyaneus and colon bacillus.

留取处理血液200～300 ml，从中取血样5 ml进行细菌培养，在余血中加入金黄色葡萄球菌、绿脓杆菌和大肠杆菌。

No staphylococcus aureus and bacillus pyocyaneus were seen after cultured in sample Ⅱ of group Ⅰ and group Ⅱ. But the colon bacillus was positive in sample Ⅱ of all the patients.

结果：〖HT5SS〗所有病人血样Ⅰ的细菌培养结果均为阴性；两组病人血样Ⅱ中金黄色葡萄球菌、绿脓杆菌培养均为阴性，但大肠杆菌培养全部为阳性。

Consider to make clear in recent years, second acid is calcic - Bowman does not move bacillus clinical bacterium of single born of the same parents of the detached rate that go up and verdigris holiday comparatives, have the tendency that year after year rises, the fundamental condition pathogenic bacteria that already became the infection inside the courtyard [1] , have to a variety of antibacterial content be able to bear or endure higher property of a medicine [1, 2] , for this, we are monitored and statistic analysed my courtyard to came 1998 2004 7 years a second acid is calcic - the clinical infection condition that Bowman does not change bacillus, to 14 kinds of antibacterial content be able to bear or endure property of a medicine and be able to bear or endure medical tendency, sum up a report to be as follows now. 1 material and method all data all come from 1.1 basic materials to came on January 1, 1998 my courtyard outpatient service was mixed on December 31, 2004 the specimen sending check of inpatient, via clinical microbiology the lab is fostered and classics appraisal is second acerbity calcium - Bowman does not move bacillus to affect case of illness, with clinical and commonly used antibacterial at the same time content makes the kill that medical quick detects. 1.2 bacteria appraisal is mixed medical quick experiment uses live thing VITEK32 of Mei Li dust is full automatic the bacterial appraisal of bacterial analysis system and its form a complete set gets stuck and medical quick card undertakes detecting, 1998~2000 year use GNI appraisal gets stuck, 2001~2004 uses GNI+ card.

近年探究表明，乙酸钙-鲍曼不动杆菌临床上的分离率和铜绿假单胞菌相当，并有逐年上升的趋向，已成为院内感染的重要条件致病菌[1]，对多种抗菌药物有较高耐药性[1，2]，为此，我们监测并统计分析了我院1998年至2004年七年间乙酸钙-鲍曼不动杆菌的临床感染情况，对14种抗菌药物的耐药性及耐药趋向，现总结报道如下。1材料和方法1.1基本资料所有资料均来自于1998年1月1日至2004年12月31日我院门诊和住院病人的送检标本，经临床微生物学实验室培养并经鉴定为乙酸钙-鲍曼不动杆菌感染病例，同时以临床常用抗菌药物作药敏检测的结果。1.2细菌鉴定和药敏试验采用生物梅里埃VITEK32全自动细菌分析系统及其配套的细菌鉴定卡和药敏卡进行检测，1998~2000年使用GNI鉴定卡，2001~2004使用GNI+卡。

Methods use monoclonal antibody against mammiferous hsp70 to recognize the related antigens of Bacillus pyocyaneus,Bacillus proteus,Bacillus coli,Staphylococcus aureus and Klebsiella pneumoniae with modified western blotting.

用抗哺乳类热休克蛋白（heat shock protein，hsp）70抗体识别绿脓杆菌、变形杆菌、大肠杆菌、金黄色葡萄球菌和肺炎克雷白杆菌抗原，进行免疫转印分析，测试正常豚鼠膜迷路中hsp70的表达水平。

Through this topic, we discover with the 2%NaOH-NALC digesting fluid may in 10 minute in rapid decomposition sputamentum resisting medium, during guarantee digestion effect not because the NaOH strong function will have killed the bacterium (timing control in 20 minutes), also will not affect the magnetic pellet the function; Through the application self-restraint quota simulation clinical specimen, we have compared the acid-fast stain and the pyoktanin yellow O fluorescence dye pick out limit, the pyoktanin yellow O fluorescence dyes the dark background to after-crop the obvious green bright the bacillus tuberculosis to compare in the acid-fast stain white background the red bacillus tuberculosis to accommodate strikingly, the pyoktanin yellow O fluorescence dyes picks out limits between 0.05~0.001 Mai unit, is higher than the acid-fast stain 0.05 Mai unit, the former sensitivity is high; Moreover, concentrates the function after magnetic nano particles the specimen smear, under the mirror demonstrated that its bacillus tuberculosis quantity is higher than the specimen which obviously has not passed through concentrates.

通过本次课题，我们发现用2%NaOH-NALC消化液可在10分钟内迅速裂解痰液的粘性介质，在保证消化效果的同时不会由于NaOH过强的作用杀死细菌（时间控制在20分钟内），亦不会影响磁性颗粒的作用；通过应用自制定量模拟临床标本，我们比较了抗酸染色和金胺O荧光染色的检出限，金胺O荧光染色黑暗背景下发出明显绿色亮光的结核杆菌较抗酸染色白色背景中的红色结核杆菌更容醒目，金胺O荧光染色的检出限在0.05~0.001个麦氏单位之间，高于抗酸染色的0.05个麦氏单位，前者敏感度较高；另外，经过磁性纳米颗粒富集作用后的标本涂片，在镜下显示其结核杆菌数量明显高于未经过富集的标本。

In the negative and interrogative forms, of course, this is identical to the non-emphatic forms.

。但是，在否定句或疑问句里，这种带有"do"的方法表达的效果却没有什么强调的意思。

Go down on one's knees;kneel down

屈膝跪下。。。下跪祈祷