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False positive were lowered to an acceptable rate by this protocol, therefore, a big amount of work on fingerprint to get rid of false positive clones can be avoided. When whole BAC clone containing one locus of a multi-loci probe were applied for library screening, no matter homogenic BAC or heterogenic BAC, site-specificity is higher than short DNA fragment probes.

对于无法通过小基因组填补间隙的区段，可以本物种BAC克隆直接进行染色体步移，采用抑制重复顺序杂交法能够有效抑制大量存在于植物基因组中的重复顺序的干扰，把假阳性率降低到可以接受的范围，从而避免了用大量指纹分析剔除假阳性克隆。

The adopted preparation method comprises the following steps: paper fibre or porous organism is used as a hard template; CoFeFe-LDH is synthesized in the surface and holes of the hard template and roasted to prepare the magnetic cobalt iron ferrite; as the function for fixing the houghite position is produced by the obstruction and adsorption of the hard template, the occurrence of the sintering phenomenon is greatly avoided, thereby preparing the magnetic cobalt iron ferrite with a consistent shape and even granularity.

所采用的制备方法是以纸纤维或多空有机物为硬模板、在硬模板表面及孔内合成Co 2 Fe 2 Fe 3 -LDH，再经焙烧得到磁性钴铁铁氧体，由于硬模板的阻隔和吸附而产生的水滑石位置固定作用，极大地避免了烧结现象发生；从而得到形状一致、粒度均匀的钴铁铁氧体。

Three meals Food and drink has arranged for responding to a few many meals , avoided full; Hyperpietic is often fatter , must eat low thermal energy food, general quantity of heat ought to be in 0 coke retinue in every day , 150-250 grams of staple food in every day under the control of , animal egg white and vegetalitas egg white account for 50% respectively.

三餐 饮食安排应少量多餐，避免过饱；高血压患者常较肥胖，必须吃低热能食物，总热量宜控制在每天8.36兆焦左右，每天主食150-250克，动物性蛋白和植物性蛋白各占50%。

The net that tax a bank note is avoided in its duty say in statement " be interrupted to serving because of force majeure or the network that clean out the reason with ticket incontrollable net to cause or other blemish, the net that tax a bank note does not assume any responsibility ", make consumer cannot ask the net that tax a bank note recoups a loss.

淘票网在其免责声明中称&对于因不可抗力或淘票网不能控制的原因造成的网络服务中断或其它缺陷，淘票网不承担任何责任&，就使得消费者无法要求淘票网赔偿损失。

A new eucaryotic expression system of recombinant PoIFN-γ was also constructed so that the denaturalization and refolding process is avoided. The target gene coding PoIFN-γ2 mature protein was subcloned into expression vector, transformed into Pichia pastoris, The His〓 Mut〓 phenotype transformants were screened, fermented in flasks and induced by 1％methanol. The expressed product in culture solution and sedimentation have antiviral activity on VSV and immunological activity proved by indirected immunofluorescence antibody and Dot blotting assay.

为了构建可对表达产物修饰、加工的真核表达系统，使表达产物具有自然干扰素的活性，无需变性、复性，我们将PoIFN-γ2成熟蛋白基因连接酵母整合表达载体，电转化毕赤酵母，筛选了His〓Mut〓表型转化子，摇瓶培养，1％甲醇诱导表达，培养上清和菌体裂解上清均有抗VSV病毒活性，经间接免疫荧光抗体检测和Dot blotting鉴定，重组酵母菌诱导表达产物具有免疫活性。

In third chapter, We extend Horowitz and Maldacena's proposal about black hole final state to Dirac fields and find that if annihilation of the infalling positrons and the collapsed electrons inside the horizon is considered, then the nonlinear evolution of collapsing quantum state will be avoided.

在第三章中，我们把Horowitz和Maldacena关于黑洞终态的假设推广到了Dirac场并发现如果考虑黑洞蒸发过程中落进视界的负能粒子同黑洞物质之间的湮灭，那么出射态的非线性演化可以避免。

In third chapter, We extend Horowitz and Maldacenas proposal about black hole final state to Dirac fields and find that if annihilation of the infalling positrons and the collapsed electrons inside the horizon is considered, then the nonlinear evolution of collapsing quantum state will be avoided.

在第三章中，我们把Horowitz和Maldacena关于黑洞终态的假设推广到了Dirac场并发现如果考虑黑洞蒸发过程中落进视界的负能粒子同黑洞物质之间的湮灭，那么出射态的非线性演化可以避免。

In the traditional trust evaluation methods, various hypotheses are put forward for the evaluated entities, and the inveracious recommendation of malicious customers can not be avoided, so the objectivity and creditability of the evaluation result is affected.

传统的信任评估方法对评估对象提出种种假设，不能分辨恶意客户的虚假推荐，因而影响了评估结果的客观性和可信性。

The effect of some experimental conditions including the acidification of water sample, the selection of absorption solution and gas separation on determination was discussed, which avoided the interference of iodometric method.

讨论了水样的酸化效果，吸收液选择，气体分离等实验条件对侧定的影响，避免了碘量法所产生的干扰。

The boundary condition of the isochoric combustion process can be precisely controlled. And the disadvantageous influence on the isochoric combustion of the engine is avoided. Therefore, the combustion process and the factors of the influence on it can be clearly found.

可以实现对定容燃烧过程边界条件精确控制，剔除点燃式发动机定容加热过程中众多杂乱影响因素，从而清晰地反映出燃烧过程及其影响因素的作用。

We have no common name for a mime of Sophron or Xenarchus and a Socratic Conversation; and we should still be without one even if the imitation in the two instances were in trimeters or elegiacs or some other kind of verse--though it is the way with people to tack on 'poet' to the name of a metre, and talk of elegiac-poets and epic-poets, thinking that they call them poets not by reason of the imitative nature of their work, but indiscriminately by reason of the metre they write in.

索夫农 、森那库斯和苏格拉底式的对话采用的模仿没有一个公共的名称；三音步诗、挽歌体或其他类型的诗的模仿也没有——人们把&诗人&这一名词和格律名称结合到一起，称之为挽歌体诗人或者史诗诗人，他们被称为诗人，似乎只是因为遵守格律写作，而非他们作品的模仿本质。

The relationship between communicative competence and grammar teaching should be that of the ends and the means.

交际能力和语法的关系应该是目标与途径的关系。