查询词典 avidin

To explore whether peptide could be used to deliver agent to target site, synthesized peptide was chemically conjugated with large molecule-BSA and NHS-d-Biotin easily detected by avidin.

目的：研究从噬菌体肽库中筛选到的与血管内皮生长因子受体Ⅱ有特异结合活性的小肽，做为KDR靶向药物的先导物质的应用。

Preliminary studies on affinity isolation based on the specific combination between Biotin and Avidin Presently, affinity chromatography is increasing to be a main protein isolation and purification technique for a complicated system because of its high specificity, high rate of recovery, etc.

利用生物素和抗生物素蛋白间的特异性结合作用，将生物素通过化学反应键合到蛋白质的某种官能团上，酶解后的肽段通过固定了抗生物素白蛋白的亲和分离凝胶色谱柱，可以选择性地分离生物素化的肽段，达到简化复杂体系的目的。

Its principle is as follows:red blood cells are labeled with NHS-Biotin,participated in the circulation and ages in vivo.Using the high affinity between biotin and avidin,the labeled RBCs with specific ages are separated.

其基本原理是用生物素标记一定量的红细胞，使之参与血液循环，并在体衰老，利用biotin与抗生物素蛋白之间的高亲和力，分离带有标记的、具有特定年龄的红细胞。

Bcl|2 and bax were studied by strept avidin|biontin complex immunohistochemistry.results the expression rate of telomerase in breast cancer was significantly higher than that in precancerous breast(p＜0.01) and in breast benign tumors.

用原位杂交方法检测端粒酶的表达及其活性，采用免疫组织化学技术sabc法检测bcl|2和bax。结果端粒酶在乳腺癌中的表达率明显高于癌前病变（p.01）和乳腺良性肿瘤。

Methods The expression of hTERT and Rb Protein was measured in formalin-fixed, paraffin-embedded gastric cancer specimens from 50 patients in gastric cancer group, 25 patients in precancerous lesions group, and 11 healthy people in normal control group by using the strept avidin biotin peroxidase method, and their correlations to clinicopathologic features were analyzed.

应用免疫组化法检测了50例胃癌标本，25例胃癌前病变组织(萎缩性胃炎伴肠上皮化生10例、萎缩性胃炎伴异型增生15例)hTERT、Rb蛋白的表达，所有病例均经病理证实。

Methods The Biotin-Strept -Avidin enzyme linked immunosorbent assay, spectrophoto-metric assay were used to measure the plasma levels of CT-1 and sICAM-1 in 56 patients with hypertension before and after treatment of Perinopril or Benidipine and 30 normal controls.

应用生物素－链霉亲和素酶联免疫吸附法对56例高血压患者应用培哚普利和贝尼地平治疗前后及30名正常健康人，测定血浆CT-1水平和血浆sICAM-1水平。

Methods The avidin-biotin-peroxidase complex method and imagine analysis were employed to detect neuro peptides-LI neurons in trigeminal ganglions of eight SD male rats.

SD雄性大白鼠8只，灌注固定，取双侧三叉神经节水平位连续切片，ABC法漂染，镜检，计算机图像分析。

The monoclonal antibody was produced by the method of inoculating BALB/c mice intraperitoneally with the hybridoma cells, and purified with protein A-Sepharose 4B affinity chromatography.3 Establishing of ELISA protocol to detect hBLySTwo kind of different ELISA protocols were tried to study the experiment condition of detecting hBLyS level. Streptavidin was used to indirect coating in protocal 1, but biotin-avidin system was used to enlarge the final reaction signal in protocal 2. The optimal reaction condition was chosen by the experiments. The sensitivity of protocal 1 was poor, but the linear relation of protocal 2 standard curve was excellent when the concentration of hBLyS was between 3.2~400 ng/mL. So a protocal was established which sensitivity can reach to nanogram.

建立检测hBLyS蛋白含量的ELISA方法选用2种ELISA方案对hBLyS蛋白含量的测定方法进行研究，方案一用链霉亲和素做间接包被，方案二用生物素-亲和素系统做终反应放大；优化选择2种检测方法的反应条件；方法一的敏感度较低：方法二在hBLyS蛋白浓度为3.2～400 ng/mL范围内，标准曲线的线性关系良好；从而建立了一种可检测hBLyS蛋白浓度为ng级水平的ELISA方法。

Methods VDR and PCNA expression was evaluated in 6 specimens of normal parathyroid tissue and 7 cases of SHPT(19 parathyroid glands) with immunohistochemistry (avidin-biotin complex method).Results The weight of parathyroid was 16.1 times greater in SHPT patients than normal subjects.

7例严重SHPT病人的甲状旁腺 19枚，正常对照6枚，用免疫组化ABC法检测维生素D受体、细胞增殖核抗原表达。

What would he tell Judith and the children?

他将怎样告诉朱迪丝和孩子们呢？

I this is at that time, the opinion with peacockish true girl is full of in a heart.

这就是当时的我，一个心中布满虚荣的女孩子真实的想法。