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Avian leukosis virus Subgroup J is a retrovirus which infects meat-type chickens.

J亚群禽白血病病毒是90年代由英国鉴定出来的ALV新的亚群,主要引起肉鸡的骨髓瘤白血病。

Key words: broiler;avian leukosis;pathological diagnosis;p53 gene;Bcl-2 gene

肉鸡;禽白血病;病理学诊断;p53基因;Bcl-2基因

The effect of an artificial infection with a China isolate(NX0101 strain) of avian leukosis virus subgroup J on the growth of commercialbroiler chickens was analysed.

此外还通过剖检和免疫器官的病理组织学变化进一步验证ALV-J对肉鸡的致病性。

Reticulendotheliosis virus ; Avian leukosis virus subgroup J ; serum index ; body weight suppression

网状内皮组织增生病病毒; J亚群白血病病毒;血清指标;生长抑制

Broiler ; subgroup J avian leukosis virus; sequence compari

肉鸡; J亚群白血病病毒;序列比较

Avian leukosis virus Subgroup J; gp85 gene ; Mutation tendency

J亚群白血病病毒;中国野毒株; gp85基因;变异趋势

ConclusionComparisons of SD07LK1 sequence with that of the other Avian leukosis viruse strains, by using DNAstar software, demonstrated that the genes gag and pol of ALV-J were relatively conservative, the nucleotide identity of all the strains was over 95.0%. However, the gene env identity was only in the ranges between 88.6 and 94.0%.

将该序列与另外已完成的全基因组序列的比较表明,ALV-J的整个基因组gag和pol基因相对保守,各毒株间对应基因的同源性分别在95.0%以上,env基因的同源性仅为

By the anatomy of the six chickens, the majority of liver, spleen and other organs significantly enlarged were found, and some could see the surface of gray nodules of various sizes, a common feature of lesions in the sternum with the face, ribs, etc appeared Soy bean to Vicia bean size white tumor-like nodules, suspected Avian Leukosis Virus-J Subgroup.

经对6只病鸡解剖发现,大多数肝、脾等内脏器官明显肿大,有的表面可见大小不一的灰白色结节,共同的特征病变是在胸骨内面、肋骨等处出现黄豆至蚕豆大小的白色肿瘤样结节,疑似禽J-亚群白血病。

To determine the extent of avian leukosis virus infection in China in 2009, 178 suspected ALV samples were collected from 39 chicken farms in eight provinces and examined by polymerase chain reaction with ALV-A, ALV-B and ALV-J gene specific primers.

为了解自2009年年初以来国内一些地区禽白血病流行情况及流行毒株的分子特征,我们从湖北、黑龙江、山东、辽宁、吉林、广东、宁夏、安徽8个省区39个鸡场采集疑似禽白血病病料样品178份,用ALV-A、ALV-B和ALV-J特异性引物,通过PCR方法进行检测。

An subgroup J avian leukosis virus,designated as SCAU-0901,was isolated and identified by histopathological observation,DF-1 cell culture,ELISA test,PCR and immunofluorescence assay specific to ALV-J from Mahuang broiler breeder suspected with ALV infection.

在组织病理学观察的基础上,经接种DF-1细胞系、ELISA抗原检测、聚合酶链式反应以及亚群特异性免疫荧光试验,从送检的疑似禽白血病感染的麻黄肉种鸡中分离并鉴定出1株J亚群禽白血病病毒,命名为SCAU-0901。

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