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The result indicated: antibacterial concentration of inner thallus increased progressively with time prolongation when added CCCP with antibacteria, proved that multi-resistance of E.coli in livestock and avian is mediated really by active efflux system; The multi-resistant level have positive correlation with AcrAB transcription and expression level; Quantitive RT-PCR,Western-blotting and fluorescence quantitive PCR can use to detect AcrAB.these results provide theory foundation to detect and study multi-resistance of E.coli in veterinary clinic and have a important scientific significance to develop neotype antibacterials.

结果表明,多重耐药株在有CCCP 存在时,菌体内抗菌药物的浓度随时间的延长而递增,证明畜禽大肠杆菌多重耐药性确实由主动外排系统介导;其多重耐药水平与AcrAB的转录、表达水平呈现正相关;定量RT-PCR、Western-blotting、荧光定量PCR可用于AcrAB水平的检测。这些结果对检测临床大肠杆菌的多重耐药水平、对研究兽医临床大肠杆菌多重耐药机制提供理论依据,对开发新型抗菌药物有重要的科学意义。

It was used for detection of virus RNA in AIV infected chicken embryo allantoic fluids clinical specimens from various avian.

建立并优化了检测禽流感病毒核酸的探针杂交法,探针杂交法能鉴别出非免疫鸡胚和SPF鸡胚尿囊液中的病毒,攻毒后第 3天的 SPF和非免疫鸡泄殖腔拭子中 AIV的最大检出率为 1/10 ,对临床样品中的 AIV的最大检出率为 1/7,而直接 HA和 HI法及 AGP试验检不出临床样品的 AIV。

The avian influenza virus RNA was directly extracted and purified from chicken em-bryo allantoic fluid. According to published gene sequence,a pair of specific primers to the nucleoprotein gene of AIV was designed and synthesized. After that,the NP cDNA was amplified by RT-PCR .Then the complete NP gene was sequenced.

直接从鸡胚尿囊液中提取H9N2亚型禽流感病毒的RNA,并根据已发表的A型流感病毒株的核苷酸序列,设计了1对特异引物,采用RT-PCR技术成功地扩增了AIV的NP基因;将NPcDNA克隆后进行了序列测定。

There were 203 avian species in Dazhanhe Nature Reserve, of which 92 Asseriformes species and 110 non-Asseriformes species were found, and composed mainly of Muscicapidae, Anatidae and Scolopacidae.

结果表明:大沾河自然保护区共有鸟类16目39科203种,其中雀形目鸟类16科92种,非雀形目鸟类111种,种类较多的科有鹅科、雀科、鸭科、鹬科等。

Trumpeter Swans belong to the avian order Anseriformes, family Anatida, along with ducks and geese.

号手天鹅属于雁形目鸟类秩序,家庭Anatida ,随着鸭和鹅。

Jq Ez6_{0An outbreak of highly pathogenic avian influenza caused by H5N1 virus occurred in Vietnam, Japan and Korea, commencing in December, 2003. By February, 2004, it has been distributed widely in Asia and brought heavy losses. H5N1 are ball-like RNA viruses, birds can shed them via respiratory system, ocular secretions and feces, sometimes transferred to human and cause lethal infection. H5N1 patients have prominent features of reactive hemophagocytic syndrome,bronchiolar inflammation, necrosis of bronchial epithelium and organizing,diffuse alveolar damage with interstitial fibrosis.

2003年12月,H5N1型禽流感病毒在越南、日本和韩国爆发,到2004年2月,该病毒已在亚洲广泛蔓延并带来了巨大损失。H5N1是球型RNA病毒,禽类间可通过呼吸系统、眼分泌物和粪便传播,也可传染人类甚至导致死亡。H5N1感染者的特征是反应性噬血细胞综合征、细支气管炎症、支气管上皮坏死和机化、弥漫性肺泡病变和间质纤维化。

Avian influenza virus was isolated from the caecal tonsil, faeces, and kidney of the domestic ducks. The isolated virus was identified as a highly pathogenic H5N1, with a haemagglutinin proteolytic cleavage site deduced amino acid sequence of ... QREKRKKR/GLFGAIAG ... In order to determine the pathogenicity of the isolate, eight 6-week-old specific pathogen free chickens were inoculated intravenously with the virus, and all birds died within 24 h after inoculation.

病毒抗原可以从鸭的盲肠,扁桃体,肾脏及粪便分离出来,并且确定这种病毒是高致病性的H5N1,还从血凝素蛋白分解位点推断出病毒的氨基酸序列,为了了解独立群的发病机理,对8只6周龄鸡静脉注射这种病毒,结果所有的均在注射后24小时死亡。

Moreover, we we sequenced the entire mitochondrial DNA control region from 17 charadriiform species, and found that the base composition and distribution of the variable nucleotide sites within the control region were similar to other avian species, but a series of short tandem repeated motifs in the 3' end of domain III made A nucleotides and length increased significantly. Phylogenetic analysis showed that these sequences could not well resolve the relationship among genus in Charadriiformes, but within genera maybe has some valuable phylogenetic information.

此外,本研究测定了鸻形目17种鸟类线粒体调控区序列,发现在碱基组成和进化速率上与其它鸟类相似,但其3'末端的串联重复序列导致domain III长度且A含量显著增加;利用调控区序列未能较好的解决鸻形目属以上水平的分类,但对于解决属内物种间的进化关系具有一定的参考价值。

Plasmid DNA profiles analysis offer scientific reference to molecular epidemiology investigation of avian colibacillosis.

质粒DNA图谱分析为从分子水平上进行鸡大肠杆菌病流行病学调查提供了基础材料。

Avian colibacillosis induced by the experimental infection method was treated and prevented using the fermenting liquor of antagonistic actinomycetes and florfenicol.

采用人工感染诱发鸡大肠杆菌病造模,分别以放线菌发酵液与氟苯尼考进行预防和治疗试验。

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However, as the name(read-only memory)implies, CD disks cannot be written onorchanged in any way.

然而,正如其名字所指出的那样,CD盘不能写,也不能用任何方式改变其内容。

Galvanizes steel pallet is mainly export which suits standard packing of European Union, the North America. galvanizes steel pallet is suitable to heavy rack. Pallet surface can design plate type, corrugated and the gap form, satisfies the different requirements.

镀锌钢托盘多用于出口,替代木托盘,免薰蒸,符合欧盟、北美各国对出口货物包装材料的法令要求;喷涂钢托盘适用于重载上货架之用,托盘表面根据需要制作成平板状、波纹状及间隔形式,满足不同的使用要求。

A single payment file can be uploaded from an ERP system to effect all pan-China RMB payments and overseas payments in all currencies.

付款指令文件可从您的 ERP 系统上传到我们的电子银行系统来只是国内及对海外各种币种付款。