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Four sets of specific oligonucleotide primers for infectious laryngotracheitis virus, infectious bronchitis virus, Newcastle disease virus, and avian influenza virus were used in this study.

我们使用针对家禽流行性感冒病毒、传染性支气管炎病毒、传染性喉头气管炎病毒、新城病病毒的特异性引子来进行测试。

Part two, The consructon of universal probe real time PCR detecting of Avian viral disease We constructed a plasmid including a specified fragment of NDV, and optimizated reactive condition of universal probe real time PCR, and detected Infectious Laryngotracheitis Viruse, Infectious Bronchitis Viruse and

第二部分:通用探针实时荧光PCR检测禽病毒病方法的建立本部分首先构建含有NDV特定片段的质粒,以提取的质粒为模板

We have researched and developed Avian Influenza inactivated vaccine , chicken Infectious Laryngotracheitis genetic vaccine,Infectious Bursal Disease Inactivated vaccine and obtained certifications of new bio-preparations approved by Agricultural Ministry of China.

截至目前,员工总数150人,其中硕士以上12人,本科学历43人,大中专以上学历60人,中级以上职称35人,公司高层管理人员均为本科以上文化程度,中层管理人员均为大中专以上学历或中级以上职称人员。

Avian leucosis virus is one of the member of retroviruses, which has been divided into 10 subgroups of A to J.

家禽白血病(avian leucosis, AL)是由家禽白血病病毒(avian leucosis virus, ALV)引起,依ALV感染宿主的不同、病毒干扰的现象及病毒封套蛋白质上的抗原性不同,可分成十个亚群。

However, measuring p27 in the blood at ages 1 and 6 weeks old by ELISA could be used for discriminating the ALV-J-infected flocks from non-infective flocks having endogenous non-tumorigenic avian leucosis. Because the p27 of the former increases but that of the latter decreases during young ages.

然而应用ALV-J可在1周龄内鸡群中迅速传播的特性,感染鸡群早期的p27会上升,因此检测1周龄及6周龄鸡只血液p27的升高,可以与无ALV-J感染只含内源性病毒的鸡群区别。

Avian leucosis virus ; monoclonal antibody ; DAS-ELISA

禽白血病病毒;单抗;双抗体夹心法ELISA

Avian leukosis virus subgroup J ; IMC_(10200) strain ; experimental ; myeloid leucosis

J亚群禽白血病病毒; IMC10200株;实验性;骨髓性白血病

A double-antibody sandwich ELISAemploying monoclonal antibody 5G5 was developed for detection of P27 antigen of avian leucosis virus.The limit of viral antigens detected was 5?

利用原核表达的禽白血病病毒P27蛋白作为抗原制备的单抗作为包被抗体,以制的酶标兔抗P27作为酶标抗体,在国内首次建立了检测ALV抗原的双抗体夹心ELISA方法。

To explore the influence of reticuloendotheliosis virus and subgroup J avian leucosis virus infection on chicken cellular immunity,the mitogenic response to ConA of blood and spleen lymphocytes tested and analyzed with 3H-thymidine(3H-TdR) incorporation assay at different intervals after infection or co-infection of single or both viruses.

自1974年分离出网状内皮增生症病毒[1]和1991年分离出禽白血病病毒J亚群[2]以来,在我国先后分离出不同毒株的REV和ALV-J,并开展了病原学、流行病学、致病性和免疫反应以及预防诊断等方面的研究,揭示出不同的致病性,并且确认它们都可造成感染鸡群免疫抑制,成为一些

In order to discuss the interaction of viremia and antibody caused by reticuloendotheliosis and avian leucosis virus of subgroup J, REV, ALV-J and REV+ALV-J were injected to SPF chicken at 2 d separately, then tested the viremia and antibody dynamically for 7 weeks. The results indicated that compared with single infection, the dynamic viremia level of REV and ALV-J wasn't obviously different when coinfected.

为了研究禽网状内皮增生病病毒、禽J亚群白血病病毒共感染时对彼此病毒血症及抗体反应的影响,2日龄SPF鸡分别接种REV、ALV-J或同时2种病毒造成共感染,对各感染组7周内的病毒血症及抗体反应进行动态检测。

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