查询词典 autoradiographic

Results:β-L-D4A was successfully synthesized and confirmed by IR, NMR and MS. Autoradiographic bands showed similar for supernatant and intracellular HBV DNA. Episomal HBV DNA was inhibited in a dose-dependent manner. EC50 of 3 -L-D4A was 0.2 μM. The experiment of cytotoxicity gained its IC_(50) 200 μM.

结果经一系列步骤合成的化合物β-L-D4A经红外光谱、质谱和核磁共振谱鉴定正确；2.2.15细胞培养上清液病毒DNA的Southern印迹、自显影结果显示药物对病毒的抑制呈明显的浓度依赖性，计算出EC_(50)为0.2μM；细胞毒性实验显示IC_(50)为200μM。

The localization of the S-labelled AVP4-8 binding sites in the rat hippocampus was studied by using autoradiographic approach via observing the selective damages of hi_p-pocampal neurons by neurotoxins,and the developmental regulation of the hippocampalAVP4-8 receptor by pretreatment with exogenous AVP4-8 was observed.

本文利用放射自显影方法结合神经毒对海马神经元的选择性损毁观察AVP4_。结合点在大鼠海马内的分布和定位；利用外源性AVP，_a对新生大鼠的处理，观察海马AVP4_。

Using a 3H-labelled 7.3kb fragment of 18S and 28S rRNA g enes as a probe and by chromosomal in situ hybridization,it was demonstrated tha t the autoradiographic silver grains to be located along entire p+ part of the m arker chromosome.

应用3H标记的7.3kb长的rRNA基因探针进行染色体原位杂交，自显影银颗粒沿整个p+区域分布，p+上的银颗粒数是正常近端着丝粒染色体短臂上银颗粒平均数的5倍。

Pneumoniae FH strain was cloned and the sequence was analysed by M13 DNA sequencing method. Comparing the PCR product sequcence with MP M-129 strain P1 gene, we found that there are 4 bases different. This may result from the different MP DNA templates. The maximum homology is 98.8%. The result confirmed the fidelity and specificity of the amplified target DNA segment by PCP, and suggested that two categories of MP P1 gene still exist a few differences even in the conservation region. The cloning MP DNA segment was labelled by random hexanucleotide priming, after hybridization, the probe detection was completed using an anti-digoxigenin antibody alkaline phosphatase conjugate, and the substrates 5-bromo-4-chloro-3-indolyl phosphate and nitro blue tetrazolium. This hybridization system is much superior to the radioactive probe hybridization, because it is safe, easy to handle and has no limitation of decay time. The time required for colormetic detection is also much less than the corresponding autoradiographic exposure time needed to achieve similar detection limits with 32P-labelled probes. The Dig-probes could be used repeatedly, and this made them not only much convenient to use, but also lower the cost, and worthwhile to be used popularly.

将PCR产物进行重组，并将阳性重组质粒，应用M13测序系统对产物进行DNA序列分析，并与MPM-129株P1基因核苷酸进行同源性比较，发现有4个位置的碱基发生了变化，其同源性为98.8％，证实了PCR所扩增DNA片段的准确性和特异性，同时也证实了不同MP组型的P1基因即使在保守区也存在着一定的差异，将克隆的目的DNA片段用异羟基洋地黄毒苷配基用随机引物法标记制备MP DNA探针，杂交后用碱性磷酸酶标记的抗Dig多克隆抗体与杂交体反应，再用BCIP和NBT呈色，制备MP DNA探针，鉴定所扩增片段的特异性，与同位素探针比较，Dig探针不受半衰期限制，可反复使用，而且价格低廉，值得推广使用。

In the United States, chronic alcoholism and hepatitis C are the most common ones.

在美国，慢性酒精中毒，肝炎是最常见的。

If you have any questions, you can contact me anytime.

如果有任何问题，你可以随时联系我。