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The increased amount of non--exchangeable NH4^＋-N during the incubation indicated as the following tendency, namely, Eum-Orthic Anthrosols 〉 Los - Orthic Entisols 〉 Hap-Ustic Isohumisols 〉 Ust-Sandiic Entisols. Organic material influenced significantly the increased amount of non-exchangeable NH4^＋-N （P was 0.0002, 0.0004 and 0.0003 when incubated at 20 d, 50 d and 60 d, respectively）. NH4^＋-N increased remarkably when Stlpa bungeana and Medicago sativa were added compared with no addition. The contents of non-exchangeable NH4^＋-N increased significantly when added （NH4）2SO4 compared without addition at 20 d, 40 d and 60d of incubation and P was 0.0037, 0.0033 and 0.0027, respectively. It was the result that the NH4^＋-N from （NH4）2 SO4 was fixed within the soil. The increased amount of non-exchangeable NH4^＋-N increased significantly for different soils, on which different vegetation types grew before the collection of soil samples, when incubated at 20 d （P=0.0434）, but not significantly at 40 d and 60d （P=0.7378 and 0.5375）. The increased amount of non-exchangeable NH4^＋-N in the soil, on which crop straw and nitrogen fertilizer had been incorporated for a long-term period, was larger than that of no addition, but it was not significantly different among these two fertilization models. Soil clay, total N and organic matter were positively correlated remarkably with the contents of non-exchangeable NH4^＋-N, the increased amount of non-exchangeable NH4^＋-N had no correlation with soil clay, whereas it had significantly positive correlation with total N and organic matter.

培养期间非交换性NH4^＋-N的增加量均表现为以土垫旱耕人为土最大，其次是黄土正常新成土，简育干润均腐土和干湿砂质新成土较小；添加有机物料极显著影响培养期间的非交换性NH4^＋-N增加量（培养20d、40d和60d时P分别为0.0002，0.004和0.0003），表现为紫花苜蓿和长芒草土壤非交换性NH4^＋-N的增加量均极显著高于不添加有机物料的对照土壤；在培养20d、40d和60d时，加（NH4）2SO4土样非交换性NH4^＋-N的增加量显著大于不加（NH4）2SO4土样（户分别为0.0037，0.0033和0.0027），这是土壤对（NH4）2SO4中NH4^＋-N固定的必然结果；不同植被类型土壤培养20d时的非交换性NH4^＋-N增加量差异显著（P=0.0434），培养40d和60d时差异不显著（p分别为0.7378和0.5375）；长期秸秆和氮肥配施土壤非交换性NH4^＋-N增加量大于不施肥对照土壤，但差异不显著土壤黏粒、全氮和有机质与培养0d、20d、40d和60d时土壤的非交换性NH4^＋-N含量均呈极显著正相关；而非交换性NH4^＋-N的增加量与粘粒无相关性，但与全氛和有机质呈显著正相关。

The sample particles studied in this project include human red blood cells, osmotically swollen into a micro-sphere, with and without treating with N-ethylmaleimide, and at different temperatures, as well as liposomes with different DOPC/cholesterol ratio (1:1 vs.1:3) For spherical RBCs, smaller deformation were observed in samples at room temperature with NEM treatment and samples at 42 ℃without NEM treatment, compared with the deformation of the control set, namely, RBCs at room temperature without NEM treatment.

这可能是由於42 ℃为红血球细胞骨架Spectrin的型态转换温度，Spectrin由tetramer转换成dimer。推测此温度及药物作用下对Spectrin的型态转换可能造成类似的效果。同时以自行合成DOPC/胆固醇比例(1：1)及(3：1)之微脂粒重覆上述之形变实验，发现两种组合比例微脂粒形变率的差异在误差范围内。

Since Watson and Crick have brought forward the model of DNA helix structure and explain about it's reproduce mechanism in molecule level, research work concerning DNA has been continuously lucubrated. However, whether translating inheritance information and rebuilding DNA molecule via menstruating DNA sequence, or to date the most compelling gene project, chromosome protract et. al, there all need a sort of molecule scissors cleaving DNA, DNA cleavage reagent. At present, tool enzyme of cleaving DNA is mostly DNA restriction enzymes founded in 70 years, which only cleave DNA at specific 4-8 base pair sequences that are usually palindromic, so this restricts the at very fast speed developing of gene technology at certain extent.

自从Watson和Crick提出DNA的双螺旋结构并对其复制机制在分子水平上进行解释后，使得对DNA的研究不断深入，但无论是通过测定DNA的序列来破译遗传信息、改造DNA分子，还是目前最令人关注的基因工程、染色体绘制等，都需要一种断裂DNA分子的&剪刀&——DNA断裂试剂，目前断裂DNA的工具酶主要是上个世纪70年代发现的DNA限制性内切酶，但所知的Ⅱ型限制性内切酶的DNA序列识别长度较短（约为4～8个碱基对），而且一般要求回文结构，这就给飞速发展的基因工程技术带来一定的限制。

Thermally denatured DNA was preconcentrated at pretreated GCE by its adsorption in open circuit for 5min or at the potential of+0.3V for 90s, and produced two well-defined oxidation peaks of guanine and adenine residues at+0.80V and+1. 11V in pH 5. 0 phospoate buffer, respectively, but the native DNA did not produce any peaks at the same conditions.

在pH 5.0的磷酸盐缓冲液中，于+0.3V富集90秒或在开路条件下吸附富集5分钟，热变性DNA可以吸附富集在预处理玻碳电极表面，阳极扫描时，鸟嘌呤和腺嘌呤残基产生两个很好的氧化峰，峰电位分别为+0.80V和+1.11V，而在同样条件下，天然DNA却几乎不出现峰。

Dipping process: 1.5~2.0%owf, bath ratio 1:10, dip at 40-50℃ for 20min, predry at 80℃, and then cure at 170℃ for 30s, or at 150℃ for 2 to 3 min

浸染法：1.5～2.0%owf ，浴比1：10，在40-50℃浸泡20min ，脱水，80℃烘干。170℃焙烘30秒或150℃焙烘2-3分钟

Nauplii metamorphosed into protozoea I at salinities of 25 ppt and 30 ppt earlier than at 20 ppt and 35 ppt at temperatures of 25°C and 30°C, whereas no regularity in time length existed among salinities at 20°C.

在温 度25及30℃的情况下，无节幼体变态成蚤状幼体第一期所需时间，在盐度25及30ppt较盐度20及35ppt短；但在温度20℃的情况下，盐度对变态时间没有影响。

The determination of quercitin was performed on Century SIL C18 BDS (250 mm×4.6 mm, 5μm) column, using methanol-water-phosphoric acid (V:V:V=60.00:40.00:0.02) as mobile phase at a flow rate of 0.8mLmin^(-1) and UV detection at 360 nm. The determination of gallic acid was performed on Century SIL C18 BDS (250 mm×4.6 mm, 5μm) column, using acetonitrile-water-phosphoric acid (V:V:V=5.00:95.00:0.02) as mobile phase at a flow rate of 0.8mLmin^(-1) and UV detection at 272 nm.

槲皮素的测定条件为：Century SIL C18， BDS(250.0mm×4.6mm， 5μm)色谱柱，流动相为甲醇－水－磷酸（体积比为60.00：40.00：0.02），流速为0.8mLmin^(-1)，检测波长为360nm；没食子酸的测定条件为：Century SIL C18 BDS (250.0mm×4.6mm， 5μm)色谱柱，流动相为乙腈－水－磷酸（体积比为5.00：95.00：0.02），流速为0.80mLmin^(-1)，检测波长为272nm。

A steel plate having a tensile strength of at least about 930 MPa (135Ksi), a toughness as measured by Charpy V-notch impact test at least about 120 joules (88 ft-lb), and a microstructure comprising at least about 90 volume percent of a mixture of fine-grained lower bainite and fine-grained lath martensite, wherein at least about 2/3 of said mixture consists of fine-grained lower bainite transformed from unrecrystallized austenite having an average grain size of less than about 10 microns and comprising iron and specified weight percentages of the additives: carbon, silicon, manganese, copper, nickel, niobium, titanium, aluminum, calcium,rare earth metals, and magnesium, is prepared by heating a steel slab to a suitable temperature; reducing the slab to form plate in one or more hot rolling passes (10) in a first temperature range in which austenite recrystallizes; further reducing said plate in one or more hot rolling passes (10) in a second temperature range in which austenite does not recrystallize, quenching (12) said plate to a suitable quench stop temperature (16); and stopping said quenching and allowing said plate to air cool (18) to ambient temperature.

一种钢板，其抗拉强度至少约930MPa135ksi），在－40℃（－40°F）下，采用夏氏V型缺口冲击试验测定的韧性至少约120焦耳（88英尺－磅），并且其显微组织包含至少约90％体积的细晶粒的下贝氏体和细晶粒的板条马氏体的混合物，其中该混合物的至少约2／3由从平均粒径小于约10微米的未再结晶奥氏体转变而来的细晶粒的下贝氏体组成，而且，其中所述的钢板包含铁和特定重量百分比的添加元素：碳、硅、锰、铜、镍、铌、钛、铝、钙、稀土金属和镁，所述钢的制备过程为：将一钢板坯加热至一个适当的温度；在奥氏体可发生再结晶的第一个温度范围内，采用一个或多个热轧道次（10），将所述板坯轧制成板材；在奥氏体不发生再结晶的第二个温度范围，采用一个或多个热轧道次（10）进一步轧制所述板材；将所述板材淬火处理（12）至一个适当的淬火终止温度（16）；停止所述淬火处理并将所述板材空冷（18）至室温。

Five miles ahead of the retreating army went the refugees, halting at Resaca, at Calhoun, at Kingston, hoping at each stop to hear that the Yankees had been driven back so they could return to their homes. But there was no retracing that sunny road.

这些难民在军队前面五英里处行进，在雷萨卡，在卡尔洪，在金斯敦先后停留了片刻，每停一次都希望听到北方佬已被击退的消息，以便回到自己家里去，可是在这条阳光譇E热的大路上却不见有谁退回的踪影。

Second, residues in the reactive site of the inhibitor were replaced by the consensus substrate recognition sequence of furin, namely, Arg at P_1, Lys at P_2, Arg at P_4 and Arg at P_6. In addition, the P_7 residue Asp was substituted with Ala to avoid possible electrostatic interference with furin inhibition. Finally, the extra N and C terminal residues beyond the doubly conjugated disulfide loops were further truncated. However, all resultant synthetic peptides were found to be temporary inhibitors of furin and kexin during a prolonged incubation, with the scissile peptide bond between P1 and P'1 cleaved to different extents by the enzymes.

高活性、低分子量的furin酶抑制剂有可能发展成为新型的抗细菌内毒素和抗病毒感染的药物，我们以绿豆胰蛋白酶抑制剂Lys片段长链22肽为模板，设计了一系列能抑制furin和kexin活性的突变体，采用如下三种方法逐步优化：一、去除Lys片段长链三对二硫键中的一对，用Ser替换Cys，减少可能存在的二硫键错误配对；二、抑制剂的活性中心根据furin底物的专一性逐步改变，即P1、P2、P4和P6位分别变为Arg、Lys、Arg和Arg，同时为了避免P7 Asp和P6 Arg之间可能存在的相互作用，将P7位Asp替换为Ala；三、将两对二硫键形成的双环的外面5个氨基酸残基去除。

However, as the name（read－only memory）implies, CD disks cannot be written onorchanged in any way.

然而，正如其名字所指出的那样，CD盘不能写，也不能用任何方式改变其内容。

Galvanizes steel pallet is mainly export which suits standard packing of European Union, the North America. galvanizes steel pallet is suitable to heavy rack. Pallet surface can design plate type, corrugated and the gap form, satisfies the different requirements.

镀锌钢托盘多用于出口，替代木托盘，免薰蒸，符合欧盟、北美各国对出口货物包装材料的法令要求；喷涂钢托盘适用于重载上货架之用，托盘表面根据需要制作成平板状、波纹状及间隔形式，满足不同的使用要求。