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The second reaction mixture consisted of 5uL 10×PCR buffer,0.5uL 20mmol/L dNTP,1uL of primer R2(25umol/L),0.5uL Taq(2.5U),1.5uL product of the first PeR reaction, 43μL of double-distilled water,the whole system was 50μL;The reaction was run under the following conditions:DNA pre-denaturation at 94℃for 5 min;DNA denaturation at 94℃for 30 s,primer annealing at 55.6℃for 1min,extension at 72℃for 30s,for 25 cycles, and extension at 72℃for 5min.

循环的条件为：94℃变性5min，从94℃变性30s、58.8℃退火1 min到72℃延伸30s共25个循环，最后72℃延伸5 min。

The first reaction mixture consisted of 5uL 10×PCR buffer,0.5uL 20mmol/L dNTP,1uL of primer F1、R2(25umol/L),0.5uL Taq (2.5U),FTA filtering,42μL of double-distilled water,the whole system was 50μL;The reaction was run under the following conditions:DNA pre-denaturation at 94℃for 5 min;DNA denaturation at 94℃for 30 s,primer annealing at 58.8℃for 1min, extension at 72℃for 30s,for 25 cycles,and extension at 72℃for 5min.

第一轮适宜反应体系分别为：5uL的10×PCR buffer，0.5uL的20mmol/LdNTP，引物F1、R2各1uL(25umol/L)，0.5uL的Taq酶(2.5U)，含有基因组DNA模板的滤膜，用去离子水补足到50 uL。

These are beings of the great neuter species: impotent men, parasites, cyphers, who have a little land, a little folly, a little wit; who would be rustics in a drawing-room, and who think themselves gentlemen in the dram-shop; who say,"My fields, my peasants, my woods"; who hiss actresses at the theatre to prove that they are persons of taste; quarrel with the officers of the garrison to prove that they are men of war; hunt, smoke, yawn, drink, smell of tobacco, play billiards, stare at travellers as they descend from the diligence, live at the cafe, dine at the inn, have a dog which eats the bones under the table, and a mistress who eats the dishes on the table; who stick at a sou, exaggerate the fashions, admire tragedy, despise women, wear out their old boots, copy London through Paris, and Paris through the medium of Pont-A-Mousson, grow old as dullards, never work, serve no use, and do no great harm.

他们全是那一大堆无用人群的组成部分；不事生产，食人之力，一无所长，有一点地产，一点戆气，一点小聪明，在客厅里是乡愚，到了茶楼酒馆又以贵人自居，他们的常用语是&我的草场，我的树林，我的佃户&，在剧场里叫女演员们的倒彩，以图证明自己是有修养的人，和兵营中的官长争辩，以图显示自己深通韬略，打猎，吸烟，打呵欠，酗酒，闻鼻烟，打弹子，看旅客们下公共马车，坐咖啡馆，上饭店，有一只在桌子下面啃骨头的狗和一个在桌子上面张罗的情妇，一毛不拔，奇装异服，幸灾乐祸，侮蔑妇女，使自己的旧靴子更破，在巴黎模仿伦敦的时装，又在木松桥模仿巴黎的时装，顽冥到老，游手好闲，毫无用处，但也不碍大事。

Between 4-9L exposures, C2H4 has a desorption channel at 155-180K. C2H4 desorption at 130K maybe results from BrCH2CH2Br absorbs in Br atom toward surfaces, after C-Br bond scission, because C2H4 has no opportunity to touch surfaces so it desorbs at low temperature. C2H4 desorption at 210K is likely to be desorption limited that, BrCH2CH2Br docomposes to C2H4, only to desorb at higher temperature. This C2H4 desorption channel maybe comes from defect site desorption.

曝露量在4-9L之间，C2H4在155至180K的范围内也有脱附的管道。130K的C2H4脱附可能是因为BrCH2CH2Br分子以Br原子朝向表面的方式吸附，在断了C-Br键后，C2H4分子没有机会接触到表面在低温就脱附了。210K的C2H4可能是脱附所决定的，亦即BrCH2CH2Br分解后在表面形成C2H4，随后温度升高至较高温才从表面脱附，此C2H4管道也许是来自缺陷位置上的脱附。

The complete fixation of compression set of China fir is achieved after wood is treated for 20 h at 180 ℃, 5 h at 200 ℃ or 2.5 h at 220 ℃ at the presence of air and treated for about 30 h at 220 ℃ in vacuum.

研究结论为：(1)在同一种热处理方式的条件下，相同温度时，随着处理时间的延长，杉木压缩试材的回复率减小；温度越高，RS随处理时间的减小程度越大。

The results showed that 1 There are 33 kinds of pyrolysates at 300℃. 42 kinds at 421℃, 50 kinds at 500℃, 49 kinds at 600℃ and 23 kinds at 800℃ 2 Under different conditions, the pyrolysates from the extraction of Honeysuckle are aldehycies. ketones, esters and furans. Most of the pyrolysates are the important components in cigarette smoke. It offers a possibility of the quality control on the extraction of Honeysuckle by using its fingerprint.

结果表明：①金银花提取物在这一系列裂解温度下检测到的挥发性热裂解产物分别为33种、41种、50种、49种、23种；②金银花提取物裂解后产生大量醛类、酮类、酯类和呋喃类物质，这些物质是构成卷烟香味的重要物质，能改善卷烟吸味、减轻刺激性。

The Department of Main wholesale fashion jewelry at home, fashion trade兼营hand-knit h at , two m at s, mechanical gear, household gener at ors to welcome you all old and new customers to p at ronize !

本批发部主营时尚家居饰品，时尚外贸帽子兼营手工编织品，亚草席，机械齿轮，家用发电机欢迎各位新老客户光顾！

The invention relates to a nanometer bone repair material, wherein it comprises hemigydrate calcium sulfate at 100 deals, nanometer hydroxyl agustite at 10-40 deals, accelerant at 0-3 deals, manna sugar at 0-3.5 deals, and solidify liquid at 30-70 deals.

本发明提供了一种新型纳米骨修复材料，含有下列组分：半水硫酸钙100份；纳米羟基磷灰10份～40份；促凝剂0～3份，上述的骨修复材料还含有0～3.5份的甘露醇；也可以含有30份～70份的固化液。

The invention relates to a surface chemistry modified latex which can be obtained by carrying out radical polymerization in emulsion in the presence of at least one ethylenically unsaturated monomer or at least one polymer having ethylinically unsaturated residual bonds, at least one radical polymerization, at least one single, double or triple block polymer which is soluble or dispersible in water, comprising an active group at the end thereof, capable of forming a radical, and chosen from dithioesters, xanthates, thioether-thiones and dithiocarbamates.

本发明涉及具有改良表面化学性质的胶乳，它可以通过在以下物质的存在下进行自由基乳液聚合来获得：至少一种烯属不饱和单体或至少一种含残留烯属不饱和键的聚合物，至少一种自由基聚合引发剂，至少一种水溶性和/或水可分散的单嵌段、二嵌段或三嵌段聚合物，在其端部包括选自二硫酯，硫醚-硫酮，二硫代氨基甲酸酯和黄原酸酯中的能够形成自由基的活性基团。

For the first time spud drilling: at 22:00 on the December 31, 2009 using the 26 " drills, earth-moving polymer drilling fluid depth of 16.40m for the first time since the Park, the drilling, at 06:00 on January 4, 2010 to the depth of drilling 262.5m, horizons Upper.fars Group at its first drilled, January 5 Electrical Measurement finished, at 04:00 on the 6th, under 20 " cementing casing to the depth of 261.50m, water, mud back to the ground, the cement plug surface depth of 248.50 m, casing pressure test with water 10.0MPa qualified, January 8 cementing electrical measuring the quality of finish, drill cement plug at 20 o'clock on the 10th end, the end of a job opening.

下面这段中文，希望网友帮忙译为英语：第一次开钻钻进：2009年12月31日22：00使用26&钻头、搬土聚合物钻井液自园井深16.40m第一次开钻，2010年1月4日06：00钻进至井深262.5m，层位Upper.fars组第一次完钻，1月5日电测完，6日04：00下20&套管至井深261.50m固井，水泥浆返至地面，水泥塞面井深248.50m，套管清水试压10.0MPa合格，1月8日电测固井质量完，10日20：00钻水泥塞完，结束一开工作。

Breath, muscle contraction of the buttocks; arch body, as far as possible to hold his head, right leg straight towards the ceiling (peg-leg knee in order to avoid muscle tension).

呼气，收缩臀部肌肉；拱起身体，尽量抬起头来，右腿伸直朝向天花板（膝微屈，以避免肌肉紧张）。

The cost of moving grain food products was unchanged from May, but year over year are up 8%.

粮食产品的运输费用与5月份相比没有变化，但却比去年同期高8％。