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The new GV preparation method we developed allowed the best visualization of chromatin distribution after Hoechst or CMA3 staining. Our results showed that GV chromatin configurations are different among different species, and thus, while chromatin condensed into a ring around the nucleolus in porcine and bovine oocytes, no such a ring was observed in caprine oocytes; Porcine oocytes were synchronized at GV1, goat oocytes at GV3n and bovine oocytes at GVf configurations before GVBD occurred, indicating that these configurations are progressive towards final maturation rather than atresia; Changes of chromatin configurations were associated with gene activity of transcription; GV chromatin configurations were affected by the environmental changes such as serum starvation and elevated temperature.

应用该技术对猪、山羊和牛不同大小、不同健康程度卵泡及体内和体外成熟过程中卵母细胞染色质构型的变化发现：1）不同动物卵母细胞染色质构型不同；猪和牛卵母细胞染色质围绕核仁凝集形成环，但山羊卵则没有此环。2）卵母细胞在GVBD之前都同步在某一染色质构型；猪同步在GV1，山羊GV3n，牛GVf，说明这些染色质构型代表着进行性变化。3）染色质构型的变化反映基因转录活动的变化。4）染色质构型受环境变化的影响。

At 4 weeks following implantation, cells on the surface of coralline hydroxyapatite were found and connective tissues were seen in the material pores in the experimental group. Cells on the coralline hydroxyapatite were observed only in the control group. At 8 weeks, new bone formation was detected on the surface of coralline hydroxyapatite; bony tissue deposition and a few chondroid tissues were found in the pores or surrounding the pores in the experimental group. A few fibrous connective tissues were observed in the control group. At 12 weeks, abundant mature woven bone was detected on the surface of coralline hydroxyapatite; medullary cavity-like structure and vessels were found in some regions in the experimental group. No new bone or bony tissues were found in the control group.

植入材料后4周，实验组可见珊瑚羟基磷灰石表面有细胞生长，孔隙内有结缔组织长入；对照组仅见珊瑚羟基磷灰石表面有细胞生长。8周时珊瑚羟基磷灰石表面有新生骨形成，孔隙内和孔隙边缘可见骨样组织沉积和少量软骨样组织形成；对照组仅见少量纤维结缔组织长入。12周时珊瑚羟基磷灰石材料表面有较多成熟编织骨形成，部分区域可见髓腔样结构形成，并有血管长入；对照组仍未见新骨及骨样组织形成。

The highest value was found in Gracilaria conferuoides for Fe enrichment with bioaccumulation coefficient at 204875.29, followed by Nemacysti decipientis for Hg at 13800.00; and the least one was Cladophora sp. for Se at only 3.48. No individual alga had strong accumulation of every heavy metal.

各藻类对各重金属的富集系数从几倍至几十万倍不等，最高是细江蓠对Fe 的富集，其次为海蕴对Hg 的富集，最差为刚毛藻对Se 的富集，其富集系数分别为204875.29、13800.00、3.48。

The study showed the recombinant wt/mCREG protein depressed the VSMC proliferation depending on dose and the optimal concentration was 400nM;2biologic function of CREG protein and the membrance receptor mechanism:①effect on VSMC migration: the wound healing experiment showed the OB2 cells migration was slower significantly after added wt/mCREG(400Nm) in supernatant. The HITASY cells migration were very slowly and no remarkable change. The gelatinase digestion and Western blot analysis showed the matrix metalloproteinase was decreased and TIMPs was increased;②effect on differentiation: after added wt/mCREG(400nM), the expression of myocardin, SMα-actin, MHC and caldesmin were increased and that of LM-1 and FN were decreased in OB2 cells. These effects were more significant when adding wtCREG.;③effect on VSMC proliferation: Cell cycle assay and BrDU stain showed: after added the wtCREG and mCREG protein, the ratio of cell in G0/G1 phase increased to 0.5773 and 0.5572 from 0.5308 respectively in OB2 group, which increased to 0.7369 and 0.7034 respectively from 0.6297 in HITASY group;3Role of M6P/IGF2R in CREG biologic function:①ELISA and co-immunoprecipitation showed the wt/mCREG binding to M6P/IGF2R directly.②antibody blocking test: when the anti-IGF2R was added to medium at the same time with wt/mCREG at different concentration(2μg/mL、4μg/mL、8μg/mL),the effects of CREG protein which depressing proliferation, migration, secretion and promoting differentiation were blocked, which had the positive correlation to the concentration of added anti body. The studies showed two combinant CREG promoted VSMC switch to differentiation phaenotype, at the same time, depress VSMC proliferation, migration and secreting extracellular matrix.

上述实验结果证实：两种重组CREG蛋白对VSMC增殖均有剂量依赖性的抑制作用，并且相同浓度的糖基化的CREG蛋白对细胞增殖的抑制效应更为显著，最佳效应浓度为400nM；2两种重组CREG蛋白添加后对HITASY和OB2细胞生物学行为的影响：①CREG蛋白对VSMC迁移的影响：刮伤实验发现，加入最佳效应浓度的wtCREG和mCREG蛋白24h后，OB2组迁移能力下降，HITASY组无明显变化；细胞外基质金属蛋白酶-2，9(Matrix metallo-proteinase 2，9，MMP2 ，9)明胶酶电泳检测和Western blot检测结果证实，两种CREG蛋白均可以使OB2细胞合成细胞外基质MMP2，9减少，而组织金属蛋白酶抑制物(Tissue Inhibitors of Metalloproteinases，TIMPs)增加；②CREG蛋白对VSMC分化的影响：加入400nM的wtCREG和mCREG蛋白12h后，OB2细胞myocardin、SMα-actin、MHC、caldesmin表达增加，LM-1、FN表达减少；③流式细胞仪分析细胞周期和BrDU染色分析证实，加入400nM的wtCREG和mCREG蛋白后，OB2组G0/G1期细胞由0.5308分别增加至0.5773和0.5572，HITASY组G0/G1期细胞由0.6297分别增加至0.7369和0.7034；3M6P/IGF2R在重组CREG蛋白的生物学功能中的调控作用：①免疫共沉淀和免疫荧光双染色分析结果显示，CREG蛋白与M6P/IGF2R存在直接结合；②应用抗体阻断实验：将不同浓度的anti- M6P/IGF2R(2、4、8μg /mL)与两种CREG蛋白同时加入培养液中，CREG蛋白抑制VSMC增值、迁移和合成细胞外基质、促进分化的效应减弱，而且与加入anti- M6P/IGF2R浓度正相关。

Result :There was no significant difference between sexes,ages and sides.The fibrillation potential amplitude was maximum at 3 to 4 months after denervation of the posterior cricoarytenoid muscle or at 5 to 6 months after denervation of the thyroarytenoid muscle and still remained at certain level for years in some patients.Significant difference was showed in complete and incomplete nerve injuries groups,but changes of amplitude with time in incomplete nerve injuries group were small.

结果：不同性别、年龄及不同侧的纤颤电位波幅间的差异无显著性，失神经环杓后肌纤颤电位波幅均值在病程2～＜4个月时最高，而失神经甲杓肌纤颤电位波幅均值在病程4～＜6个月时最高，相当一部分患者的纤颤电位波幅晚期仍维持一定水平，并且神经完全损伤者和不完全损伤者各时间纤颤电位波幅均有显著性差异，而神经不完全损伤者各时间幅度变化小。

When no imaging/measurement is done at all at a particular time point, the patient is not evaluable at that time point.

如果在所有某一特定时间点，没有影像资料和其他测量指标，那么该患者在该时间点是不可评估的。

Skin stimulation test and implantation in vivo found no abscess and fistule formation. Histological section showed there was moderate inflammatory reaction of forign object exciation at the early stage of implantation. At the intermediate stage, inflammatory cells decreased, mainly including lymphocytes, and there was little inflammatory reaction and antigen reaction. Meanwhile, SIS degraded sequently. At 12 weeks, SIS was almost completely degraded and absorbed.

皮肤刺激试验和体内植入研究发现，无脓肿及瘘管形成，组织切片发现SIS植入后组织反应在早期呈现出异物刺激引起轻中度炎性反应，中期时炎症细胞减少，以淋巴细胞为主，炎症反应和抗原反应都较小，同时进行程序性的降解。12周时SIS几乎完全降解吸收。

Boy with eyes looking at you begging, he knew he had no feathers to the Princess to eat, but we have not been eating. More afraid of the boy, he can not afford to lose Yu Fei, he turned around in the bag, and try to find some things to eat, but ...... all of a sudden, his eyes, yes, he found the miracle is that Fei Yu-box Do not eat instant noodles, instant noodles, he immediately won, but the Gangan eat instant noodles how Fei Yu, Yu Fei did not have the strength. When the boys open the instant noodles, and there have been a miracle, even inside the meat, boys come up with a knife, chopped meat, a feather to a feed under the Princess. Another at a second time in the past, boys have been exhausted, he fell, the fourth day in the morning, the cave was opened, which re-launched into the sun, some of the passengers, including boys and girls were taken to hospital emergency treatment, because the boys Fei Yu Love left, and the boy was gone, with infinite love and comfort never left, even though he failed to accompanied Princess feather in the side, but he realized he had tears of angels and the desire to promise: I would like to Yu Fei at the expense of all.

男孩用乞求的目光看着大家，他知道自己已经没有可以给羽妃吃的东西了，但是大家也早已没有了吃的东西男孩更加害怕了，他不能失去羽妃，他在包里到处翻，希望可以找到一些可以吃的东西，然而……忽然，他眼前一亮，是的，他找到了奇迹，是那盒羽妃没有吃的泡面，他马上拿下泡面，可是干干的泡面羽妃怎么吃啊，羽妃已经没有力气了当男孩打开泡面时，又一个奇迹出现了，里面竟然有肉，男孩拿出刀子，把肉切碎，一口一口给羽妃喂下时间又一分一秒过去，男孩已经筋疲力尽，他倒下了，第四天早晨，山洞被打开了，里面重新射入阳光，一些乘客，包括男孩和女孩被医院带去抢救，羽妃因为男孩的爱留下了，而男孩却走了，带着无限的爱和宽慰永远离开了，虽然他没能陪在羽妃身边，但他实现了他在天使眼泪下的愿望和诺言：我愿意为羽妃牺牲一切

We found that younger age at onset of stroke, white-collar occupation, motor performance of hemiparesis at six months after stroke, no urine incontinence at admission were significant factors to return to work after stroke.

研究结果显示中风发生时之年龄、工作性质、中风初期是否有小便失禁及肢体障碍之程度均为统计上有意义之因子。

An antibody binding to IGF-IR and inhibiting the binding of IGF-I and IGF-II to IGF-IR which is characterized in that said antibody is a is ofIgG1 isotype, b shows a ratio of IC50 values of inhibition of the binding of IGF-I to IGF-IR to the inhibition of binding of IG-II to IGF-IR of 1:3 to 3:1, c inhibits for at least 80% at a concentration of 5 nM IGF-IR phospohrylation in a cellular phosphorylation assay using 3T3 cells providing 400,000 to 600,000 molecules IGF-IR per cell in a medium containing 0.5% heat inactivated fetal calf serum when compared to such an assay without said antibody, and d shows no IGF-IR stimulating activity measured as IGF-IR phophorylation at a concentration of 10 M in a cellular phosphorylation assay using 3T3 providing 400,000 to 600,000 molecules IGF-IR per cell in a medium containing 0,5% heat inactivated fetal calf serum when compared to such an assay without said antibody has improved properties in antitumor therapy.

一种已经提高了抗肿瘤治疗的特性的抗体，所述抗体结合IGF-IR并且抑制IGF-I和IGF-II与IGF-IR结合，其特征在于所述抗体a是IgG1同种型，b显示其对IGF-I与IGF-IR结合的抑制的IC 50 值与其对IGF-II与IGF-IR结合的抑制的IC 50 值的比率为1∶3-3∶1，c当与没有所述抗体的这种测定比较时，其在5nM的浓度上，在包含0.5％的热灭活胎牛血清的培养基中，使用3T3细胞的细胞磷酸化测定中，抑制至少80％的IGF-IR磷酸化，所述3T3细胞提供400，000-600，000分子IGF-IR/细胞，和d当与没有所述抗体的这种测定相比，在包含0.5％的热灭活胎牛血清的培养基中，使用3T3的细胞磷酸化测定中，其在10μM的浓度上没有显示作为IGF-IR磷酸化所测量的IGF-IR刺激活性，所述3T3提供400，000-600，000分子IGF-IR/细胞。

Singer Leona Lewis and former Led Zeppelin guitarist Jimmy Page emerged as the bus transformed into a grass-covered carnival float, and the pair combined for a rendition of "Whole Lotta Love".

歌手leona刘易斯和前率领的飞艇的吉他手吉米页出现巴士转化为基层所涵盖的嘉年华花车，和一双合并为一移交&整个lotta爱&。

This is Kate, and that's Erin.

这是凯特，那个是爱朗。