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This suggested there might have cis-acting element in 3'-UTR. In order to search the possible trans-acting factor, we found RNA-protein band in H〓 cells and no band in 95D cells using 3'-UTR probe by RNA EMSA assay. At the same time, this band could be competed with the unlabled probe. There was also no band obsearved using 5'-UTR probe in both cells.

为了寻找可能与3'-UTR结合的蛋白质反式作用因子，用RNA凝胶阻滞(electrophoresis mobility shift assay，EMSA)的方法，我们在H〓细胞的胞浆蛋白质提取物与3'-UTR探针的结合反应中，发现了一条RNA-蛋白质的结合带，而在95D细胞没有结合带出现；而且这种结合带能被未标记探针竞争性抑制；5'-UTR的结合反应也没有观测到结合带的形成。

At the market economy age, free, no limit and unmovable land utilization is no longer existing.

在市场经济时代，土地无偿、无限期和无流动使用的现象已彻底改变，国家法律法规明确规定，国有土地实行有偿、有限期、可以流转土地使用权等制度，这就为做好地租征缴工作提供了法律依据。

Masked under the guise of an externally sanctioned, absolute authority-----such as the "gods"-----or an internally sanctioned absolute authority----such as "mystical" experience----the unphilosophical mind deceives itself into thinking it has arrived at some final, certain knowledge, when in truth the mind has not arrived because there is no arrival; there is no authority; there is just the ever-evolving, living path called wisdom.

以表面的装腔作势为特色的那些绝对权威，诸如"众神"，或自以为"心有所感"的那些诸如体验之类，不过都是些毫无哲学素养的自欺欺人；他们以为自己达到了某个终点，获得了某些绝定不错的道理；而其内心实在无所得的时候，却又认为"本应无所得"；这世界上从来没有权威，有的只是不断的进化过程和我们自己的生活之道，它的名字叫智慧。

Your boss, no matter how narrow - minded , no matter how unprepossessing, but his manager, after all, he is sitting at the table.

你的上司无论多狭隘，无论多不讨人喜欢，可他毕竟是管理者，他是坐在餐桌上的。

Cell proliferation and viability were assayed 48h after transfection, and MDA-7 demonstrated selective inhibition of tumor cell growth, inhibitory rates of A549, Hela and HepG2 cell lines were 25%, 20% and 19%, respectively, but had no significant effect on human fetal kidney derived 293 cell line. Hela cell was screened by G418 for 2 weeks after pcDNA3-MDA-7 and monolayer colony was counted, its monolayer colony formation was 30% of cells transfected with pcDNA3. 0 vector. CMV-driven MDA-7 adenovirus vector was constructed. 293 showed no significant apoptosis during adenovirus packaging and the unpurified adenovirus titer was about 1×10〓pfu/ml. Cos 7, A549, Hela, HepG2 and Hep3B cell was infected with Ad-GFP at different MOI.

二。黑色素细胞分化相关蛋白-7（MDA-7）的克隆及功能研究：利用RT-PCR方法从5月龄人胚胎脾细胞扩增MDA-7的编码序列，经测序鉴定序列与文献报道一致后，与真核表达载体pcDNA3.0连接，构建pcDNA3-MDA-7表达载体，瞬时转染293、A549、Hela和HepG2细胞后抽提细胞总RNA，RT-PCR结果显示表达载体可介导MDA-7在不同细胞系中有效表达；转染48h后测定细胞增殖和活力，MDA-7可选择性抑制肿瘤细胞的增殖，对A549、Hela和HepG2细胞的抑制率分别为25％、20％和19％，但是对人胚肾来源的293细胞生长无明显影响。pcDNA3-MDA-7载体转染Hela细胞后，以G418筛选2周后计数单层细胞集落形成数，计数仅为转染pcDNA3.0空载体的细胞的30％左右。

He considers his whole life as a single act,as if he boards on a train that has no end.He paints with fine paints,sits on the chairs of the old generations.He grows from a utility man to a leading actor,migrates from Japan to China.He stops at each station,but no stops are endpoints for him.

"这句很美，我就先在这里翻译了，"他用一生的时间演一场戏，如同登上一列没有终点的地铁，涂着精致的油彩，坐着前辈的椅子，从龙套到主角，从日本到中国，每一站都会停留，每一次停留都不是永远。

But you will be making a big mistake if you think it will happen this time: there will be no V-shaped recovery; perhaps no recovery at all.

但是， 你将成为一个很大的错误，如果你认为它会发生这个时候：将不会有V型复苏；也许没有恢复所有。

There is no wonder that human is a kind of strange animal,I remember a famous author is named 'MaoDun',on the other hand,it is said that to be a human sometimes is equal to be many inconsistencies, maybe today we laugh at a person ,just cause we deem him who always do something stupid,but many years later,when we are remind of such a person,we have to admit that the veriest stupid person are just oueselves,a proverb said by a former philosopher is that,still water runs deep ,but the world is no longer the world our philosopher had mentioned about,the world is becoming smaller and smaller,what about you...

这也难怪，人本来就是一个奇怪的生物，有个著名作家不是就叫茅盾么，说明做人有时是很矛盾的，也许现在我们因为他做一些自己看来很傻的事情而称他为傻子，然而若干年后再提起他时，我们却不得不暗骂自己是傻子，圣贤书中曾有云，大音如希，大象于无，大智若愚，可是这个世界再也不是圣人提及的那个世界了，地球变小了，你能不变么。。。

NO streaming protocols are supported at this time, so NO live content via MMS and other media servers.

没有流协议的支持，在这个时候，所以不会有活的内容透过MMS和其他媒体服务器）。

Ask for help the ASP WEB the application the procedure, the customer passes the Browser software conjunction backstage database and complete to do the operation, on the B/ the mode of S net shopping system, the data supports the convenient customer carries to have no to need the appropriative software, having the good network expand the sex, supporting the TCP/ the IP agreement connects into the Internet directly, at the same time for B/ the mode of S but speech, the procedure have been no longer so important with the physics position of the data, the visitant concern just their URL all right.

借助 ASP 的 WEB 应用程序，用户通过Browser 软件连接后台数据库并完成做操作， B/S模式的网上订餐系统，数据维护方便客户端无需专用的软件，有较好的网络扩展性，支持TCP/IP协议直接接入Internet，同时对于B/S模式而言，程序和数据的物理位置已经不再是那么重要了，访问者所关心的只是它们的URL就可以了。

According to the clear water experiment, aeration performance of the new equipment is good with high total oxygen transfer coefficient and oxygen utilization ratio.

曝气设备的动力效率在叶轮转速为120rpm～150rpm时取得最大值，此时氧利用率和充氧能力也具有较高值。

The environmental stability of that world - including its crushing pressures and icy darkness - means that some of its most famous inhabitants have survived for eons as evolutionary throwbacks, their bodies undergoing little change.

稳定的海底环境─包括能把人压扁的压力和冰冷的黑暗─意谓海底某些最知名的栖居生物已以演化返祖的样态活了万世，形体几无变化。