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The results showed that POD activity increased at first and then decreased in the skin and flesh of root. The peak occurred at 40 d and 60 d in the skin and flesh, respectively. POD activity in the skin was higher than that in the flesh at 30 d and then contrary results were observed. The higher POD activity was, the more POD isoenzyme was. The pattern of POD isoenzymes was different between skin and flesh at different vegetative growth stages. POD isoenzymes in the skin were composed of five monomers and four dimers while five dimers or two monomers, two dimers and one tetramer in the flesh. POD isoenzymes in the root were mainly acidic. POD localized mainly in the cell walls of periderm, cambium, xylem ray, xylem vessels and primary xylem.

结果显示,心里美萝卜营养生长期肉质根的皮和肉中POD活性均先升高后降低,分别在播种后第40 d和60 d出现峰值,30 d时皮中POD活性高于肉,之后则相反,酶活性最高时同工酶数目也最多,不同时期,皮和肉中同工酶种类不同;皮中POD同工酶由5个单体和4个二聚体组成,而肉中由5个二聚体或2个单体、2个二聚体和1个四聚体组成,均以酸性同工酶为主;组化定位显示POD主要分布在肉质根的周皮、形成层、木射线、木质部导管和肉的初生木质部等的细胞壁附近。

The results were as follows:(1) The spectral sensitivity response at 14 monochromatic light selected through 340nm to 605nm occurred in curve with multiple peaks, the primary peak in 340nm (ultra-violet) is the highest response rate of phototaxis (21%), the secondary in 524nm, and the rest in 400-440nm and 483nm in order;(2) The response rate of phototaxis is increasing as the relative intensity of light, it is showed as curves with inverse "L" shape in white light and the monochromatic light of 483nm, as an upward ladder at 524nm and a near straight line at 340nm. However, the response rate could be detected at the lowest intensity and not arrived to the summit at the strongest yet;(3) The effect of sex to response to the spectral sensitivity and intensity is not significant;(4) There was not significant photophobism response to light for the ladybird, the behavior probably derived from phototaxis or partly due to random movements. It can be concluded that both the spectrum and intensity of light stimulus gave rise to the phototaxis of P.

结果显示:(1)在340~605nm波谱内其光谱趋光行为反应为多峰型,峰间主次不明显,紫外340nm处峰最高,趋光反应率达21%,其它各峰依大小次序分别位于绿光524nm、蓝光400~440nm和483nm处;(2)随光强增强其趋光反应率增大,白光和483nm刺激时其光强趋光行为反应呈一倒"L"型式样,524nm时呈一阶梯型,340nm时为一较平直的线型;光强最弱时仍均有一定趋光率,最强时均未出现高端平台;(3)性别对其光谱和光强度行为反应均有一定影响,但影响不大;(4)龟纹瓢虫无明显的避光反应,其避光行为可能是趋光行为衍生或其随机活动造成。

Major results were summarized as follows:(1) A total of 58 tree species from 53 genera in 36 families among which 8 species were Rosaceae, 5 species were Liliaceae, 5 species were Eriaceae, 3 species were Ranunculaceae, 3 species were Compositae, 2 species were Cupressaceae, 2 species were Caprifoliaceae, 2 species were Umblliferae, and other 28 tree species were only one receptively. These were recorded in the 8 plots of the A. georgei var. smithii forest;(2) Number of families, genera, species and Margalef index correlated negatively with altitude (P.05), with a peak at 3600 m.(3) Shannon-Wiener index correlated negatively with altitude (P.01), and maintained stable at the altitudes between 3700~4100 m, evenness with altitudes, however, this trend was insignificant.(4) Jaccard index increased sharply with increasing altitude at the altitudes between 3600~4100 m, and was lower between different vegetation types at the altitudes between 4100~4200. Cody index β(subscript c decreased with an increasing altitude, but there were 2 troughs between 4000~4100 m and 4200~4300 m.(5) Maximum tree height H(subscript max and HH(subscript max=37 mcorrelated negatively with altitude (P.05); but basal area and BA BA(subscript max=5.3m^2 correlated with altitudes, however, this trend was insignificant.

结果表明:(1)在调查的8个急尖长苞冷杉林样地内共有植物58种,分属于36科53属,其中植物较多的科有蔷薇科8种、百合科5种、杜鹃花科5种、毛茛科3种、菊科3种、柏科2种、忍冬科2种、伞形科2种,其余的28科各只有1个种;(2)物种科、属、种数、Margalef指数D(下标 M与海拔存在显著的负相关性(P.05),在分布急尖长苞冷杉最低海拔3600m处出现物种丰富度的最大值;(3)多样性指数与海拔之间有极显著的负相关性(P.01),并且在3700~4100m之间多样性指数保持稳定;均匀度指数与海拔梯度之间存在负相关性,但不显著;(4)Jaccard指数C(下标 j在海拔3600~4100m随海拔的升高而升高,在生境过渡带的4100~4200m之间Jaccard指数C(下标 j较低;Cody指数β(下标 c随海拔的升高呈下降的趋势,但在4000~4100m和4200~4300m 海拔区间出现2个低谷;(5)最大树高H(下标 max和最大胸径DBH(下标 max与海拔之间存在显著负相关性(P.05);胸高断面积之和和立木密度与海拔之间存在负相关性,但不显著。

A scheme to be formulated and submitted for approval to the harbour commissioners for the exploitation of white coal, obtained by hydroelectric plant at peak of tide at Dublin bar or at head of water at Poulaphouca or Powerscourt or catchment basins of main streams for the economic production of

537有个建造水力发电厂的计划:利用都柏林沙洲的满潮、噗啦呋咔或鲍尔斯考特瀑布的水位差、主要河流的流域来开发白煤,经济生产五十万水马力的电力。

Transcript level was not obviously different between MSTN and IGF2. The expression of MSTN reached the highest point at 7 day and droped to the lowest point at 7 weeks after birth. The expression of IGF1 arrived at the highest peak at brith, and degraded the half of the birth after 7 days. Then, the change of the IGF1 expression level was no abvious. The expression result of the IGF2 decreased to the least at 1 week and increased the max point at 3 weeks. The change regulation of IGF2 expression was showed as a wave.

其中,IGF-1表达水平最低,MHC2a表达水平最高;IGF2与MSTN表达水平相当; MSTN的表达于7天时表达水平最高,是出生时表达的3倍,随后于14天时急剧下降,于7周时降至最低水平;出生时IGF1的表达水平最高,随后于一周时降至出生时的一半,后期的表达变化不大; IGF2的表达水平于3周时表达最高,是出生时的两倍,在1周时最低。3周后骤然降低,呈现一个波浪型变化;MHC的表达出生时最高,于1周后降至原来的1/7,随后逐渐升高,但变化并不太大。

The biomechanical tests showed that two kinds of artificial bones had not significant difference on compressive strength and Young\'s modulus(P>0.05),while the flexural strength of nano-nacre artificial bone was less than the control group(P<0.05).3.The results of CCK-8 showed that the difference were not significant in each group,the proliferation of osteoblast reached the peak at the 5th day;7 days after being co-cultured,the total protein content of study group was higher than control group and blank group(P<0.05),while the difference between control group and blank group was not significantP>0.05The difference of alkaline phosphatase activities among three groups was not significant(P>0.05The SEM view showed that osteoblast attached and grew well in two kinds of artificial bone.4.X-ray photography showed that two kinds of powder started to degrade in 2 weeks;this phenomenon became more appear in 4 weeks,nano-nacre powder degraded faster than micron-nacre powder,while the hole shadow was easy to be found;in 8 weeks,all the femoral holes recovered and returned to normal bone mineral density in all groups.Analysis of tetracycline fluorescent double marks in the hard tissue grinding slices indicated that new bone grew fastest around the bone defect area in study group,while most slowly in blank groupP<0.05 SEM(scanning electron microscope observation showed that nano-nacre powder degraded more quickly.The same result can be found through the demineralized sections morphometric analysis,and both of the composite artificial bones made from those two kinds of nacre powder had the good connection with the adjacent tissue in rats body without apparent inflammatory response.5.X-ray photography showed that rabbit\'s bone defects healed faster in study group since NNAB implanted than in control group since MNAB implanted.At 24 weeks after operation,bone density in radial defects had nearly accessed to the normal area,while lower in control group,and turned up nonunion in blank group;The checking of BMD showed that results in study group were higher than those in control group at 8,16 and 24 week(P<0.05), and the difference between the BMD values in study group at 24 week and those in blank group was not significant(P>0.05).The gross specimens showed satisfactory histocompatibility both in study group and in control group,with bone tissue growing from two sides into the center of implanted materials; Normal slices in HE stain and hard tissue grinding slices in Stevenel\'s blue/Van Geison\'s picro-fuchsin stain showed that the bone growth tendency was better in study group than that in control group,and the medullary cavity had been penetrated to the implanted materials in study group at 24 week;Analysis of tetracycline fluorescent double marks in the hard tissue grinding slices indicated that new bone in both groups grew fastest 8 weeks after surgery,while slow down at 16 week.

纳米珍珠层/消旋聚乳酸复合人工骨与微米珍珠层/消旋聚乳酸复合人工骨分别与成骨细胞共培养后,其各时间点CCK-8法检测值与空白对照无显著差异(P>0.05),成骨细胞均在第5天达到增殖高峰期;培养7天后,实验组细胞蛋白含量高于对照组及空白组(P<0.05),后两者之间则无显著差异P>0.05碱性磷酸酶活性在三组间均无显著差异(P>0.05电镜下可见成骨细胞在两种人工骨上都有良好生长贴附能力。4.X-ray显示两种粉体在大鼠股骨骨洞植入第2周时都开始出现了降解,第4周时更为明显,纳米珍珠层粉较之微米珍珠层粉降解更快,而空白对照组骨洞阴影仍可见,至8周时,则所有组骨洞均己闭合修复,X-ray下已不可见原钻孔痕迹,恢复正常骨质密度;硬组织磨片四环素荧光双标记结果显示纳米珍珠层粉植入组较其余两组在骨缺损区周围新骨生长速度更快,空白组速度最慢P<0.05电镜观察及常规脱钙切片亦可见到纳米粉体降解较快;由以上两种原材料制得的纳米珍珠层/消旋聚乳酸复合人工骨与微米珍珠层/消旋聚乳酸复合人工骨在大鼠体内均与周围组织结合良好,无明显炎症反应。5.X-ray显示纳米珍珠层/消旋聚乳酸复合人工骨植入兔桡骨缺损区后其骨愈合速度较对照组微米珍珠层/消旋聚乳酸复合人工骨植入的快,至植入术后24周,实验组骨缺损区接近正常骨密度,对照组骨缺损区密度较低,空白组则呈现骨不连状态;骨密度测量结果显示术后8周、16周、24周实验组的骨密度值高于对照组(P<0.05,24周实验组的骨密度值与术前所测得的正常值无显著性差异P>0.05动物取材大体所见均显示组织相容性良好,骨组织逐渐由植入材料两端向中央生长;常规切片HE染色及硬组织磨片Stevenel\'s blue/Van Geison\'s picro-fuchsin联合染色均可见实验组骨缺损区长势优于对照组,至术后24周,实验组骨髓腔与材料已呈相交通状;硬组织磨片荧光显微镜下观察,两组材料在术后8周处于骨生长最快速时期,16周时速度开始减慢,术后4、8、16周时实验组的新骨生长速度均较对照组的快

Egr-1 mRNA and Egr-1 protein hadn't been found in the normal vein. The expressions of Egr-1 mRNA and Egr-1 protein had biphasic changes. By reverse transcription-PCR and in situ hybridization, we found that the level of Egr-1 mRNA rose at 1 hour after graft, the expression of Egr-1 mRNA was (35±7)%. Decline at hour 6, 24 and day 3, the positive rates of Egr-1 mRNA were (8±2)%,(8±6)% and (8±4)% respectively. Reincrease at day 7, a peak at day 28, the positive rate of Egr-1 mRNA was (45±6)%(compared with other phase, P<0.01). At day 42, the expression of Egr-1 mRNA declined again. Immunohistochemical staining and Western blot revealed Egr-1 protein had expressed at hour 2 early phase, the expression of Egr-1 protein was (30±5)%, and until to hour 6. The level of Egr-1 protein was decrease at hour 24 and day 3, the positive rates were (7±3)% and (7±8)% respectively.

结果 自体静脉移植后,Egr-1 mRNA和Egr-1蛋白的表达呈双相变化,即移植后1 h, Egr-1 mRNA表达迅速升高,阳性率为(35±7)%,6 h、24 h及3 d时下降到较低水平,阳性率分别为(8±2)%、(8±6)%和(8±4)%,7 d时又再升高,28 d时达高峰,阳性率为(45±6)%,此与其余各时点比较差异均有统计学意义(P<0.01),42 d时,Egr-1 mRNA的表达再次下降;移植早期(2 h)即有Egr-1蛋白的表达,阳性率为(30±5)%,并持续至6 h,24 h~3 d表达下降到较低水平,阳性率分别为(7±3)%和(7±8)%,7 d时又再升高,至移植后28 d,Egr-1蛋白的表达阳性率达到高峰,为(40±9)%,此与其余各时点比较差异有统计学意义(P<0.01)。

A correlation of the experimentaldynamic response of the vessels with the theoretical finite element calculations ispresented and discussed.1 The response of the end caps is dominated by transverseand torsional deformation of the plates.The membrane strain is negligible compareto the lateral strain.2 In the lengthwise direction of the inner cylinder,themembrane strain has the same magnitude as that of the bending strain. But for theouter cylinder,the former is much smaller than the later at the same direction.3 Forboth the inner shell and the outer shell,hoop strains are primarily due to themembrane deformation.The amplitude of the circumferential flexural strain isconsiderably small.4 Peak strains in the vicinity of the connection portion of theend caps and the shells are very high.This is important to assess the safety of thecontainer in case of repeated use or tests.5 The applying load to the end cap isdifferent from that to the inner shell plate, but peak stains and nodal displacementsof these two structural parts are nearly the same.It can be inferred that the dynamicresponse of the end cap is greatly affected by the structural vibration excited by theimpulsive loading.

结果表明:1在本文所考虑的载荷水平下,平板变形以板的横向弯曲和扭曲为主,中面应变幅值比较小。2对于内容器,其纵向变形中弯曲应变和中面应变大小接近;而对于外容器,其纵向变形以弯曲为主,中面变形比较小。3内、外壳体的环向变形主要是中面应变,弯曲应变分量很小。4平板和壳体连接处应变很大,这对于多次使用或实验时评价结构安全有指导意义。5尽管内筒底板和大封头上的作用载荷有一定差别,但应变峰值和节点位移峰值基本相近,这说明脉冲载荷激发起来的结构振动对封头的动态响应有很大影响。

In order to let more people understand Qinghai, know Qinghai, give culture of Tibet of Qinghai, mysterious show of characterized Tibetan medicine in Qinghai-Tibet Plateau to common people at the same time, the mauve salt people in Qinghai advocate natural aspiration to the mankind very much, have used the struggle for several years too and has been " woken with striving up" and " mysterious treasure " for 380 million years under the ice sheet of Qinghai-Tibet Plateau is buried deeply, it peak favor in mankind of " the roof of the world ", Qinghai mauve salt people aim of own duty in line with " reciprocate society, reciprocate naturally, reciprocate every consumer ", make unceasing progress, enable peak real benefit and the mankind of " mysterious material " of 380 million years of " the roof of the world ".

为了让更多人了解青海、认识青海,同时把青海的藏文化、青藏高原的特有藏药之神秘展示给世人,青海紫红盐人极至了人类崇尚自然的渴望,也用数年的奋斗与拼搏&唤醒&了深埋在青藏高原冰层下三亿八千万年的&神秘宝藏&,它是&世界屋脊&之巅对人类的恩惠,青海紫红盐人将本着&回报社会、回报自然、回报每一位消费者&为己任的宗旨,不断进取,使&世界屋脊&之巅三亿八千万年的&神秘物质&真正的造福与人类。

The experimental results show that: DNA of normal gastric mucosa has stable phosphate backbone; Since the peak intensity at 1090 cm^(-1) is lower than that at 1050 cm^(-1), phosphate backbone in DNA of intestinal metaplasia becomes unstable, while the rest spectra peaks are similar to those in normal gastric mucosa; In terms of adenocarcinoma DNA, double peaks at 1090 cm^(-1) whose intensity are higher than 1050 cm^(-1), are observed, which indicates that DNA chain is broken and re-forms a double stabilized phosphate backbones. In addition, peaks at 950 cm^(-1), 1010 cm^(-1), 1100~1600 cm^(-1) also exhibit obvious difference compared with normal gastric mucosa, which shows that deoxyribose and bases are changed due to broken of DNA.

实验结果表明,胃粘膜正常组织DNA中有稳定的磷酸骨架;肠化组织基因组DNA拉曼特征峰在1090 cm^(-1)处谱峰强度低于1050 cm^(-1)处谱峰强度,表明其磷酸骨架变得不稳定,其它位置的谱峰与正常组织相似;腺癌组织基因组DNA拉曼特征峰在1090 cm^(-1)附近出现双峰,其相对于1050 cm^(-1)处谱峰强度更强,提示DNA有可能出现断链并再次形成了稳定的磷酸骨架,在950 cm^(-1)、1010 cm^(-1)、1100~1600 cm^(-1)波段的特征谱峰与正常组织DNA相比变化也较大,说明脱氧核糖和碱基可能由于DNA断链也随之改变。

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