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The nanoplex were smaller than the unmodified nHAP; thelipiodol/nanoplex emulsion, with the mean diameter at 75±11.8nm can disperse moreuniformly and be more soluble and stable, contrary to the unmodified nHAP and nanoplex Conclusion: Nanoplex/pDNA mass ratios greater than 15 had exhibited completepDNA absorption onto positive charged polyplex and pDNA protection from thedestruction of nucleinase in rabbit serum. Unmodified nHAP may can\'t induce effectivegene delivery.PartⅡThe construction of the recombint eukaryotic expressive vector ofwild-type human p53 and RbObjective: To construct the recombint eukaryotic expressive vector of wild-type humanp53 and Rb by utilizing the theory of molecular medicine.

结果nHAP颗粒本身大小小于100nm，但由于颗粒表面本身带负电，不能包括有效地结合及保护DNA；经Ca~(2+)修饰后，颗粒可带正电，可一定程度地结合和保护DNA，但聚合物本身的中性电与带负电的细胞膜结合，而且本身的颗粒则变地容易聚集而成为很大的颗粒，也不利于细胞的吞噬；经PLL修饰后，颗粒溶解性提高，直径变小，表面带正电，电泳结果显示PLL-nHAP颗粒可包括有效地结合和保护DNA免受兔血清的破坏，而且复合物表面的正电也包括有利于与细胞膜的结合与吞噬。

Based on the researches of prehominid this thesis first discusses and analyzes on the product characteristic and the cost structure and also the factors that affecting the pricing of software. After that it gets a stage conclusion that the pricing strategy of the traditional product isn't suitable for software product. Then it analyzes the problems of the president pricing strategy of software .At last it gives an operatable and practical pricing model by the example of the ERP software product.

本篇论文在综合前人关于信息产品和软件产品定价方法的研究成果的基础上，首先对软件产品的特点、成本结构、影响软件定价的内外各种因素进行分析和理论探讨，从而得出传统的物质产品的定价方法不适用于软件产品定价这一阶段性结论；然后综合现有的软件定价方法和策略，对其存在的问题进行分析；最后运用定性分析和定量分析相结合的方法，以ERP软件为例提出一个可操作性较强的实用定价模型。

At such a rate,the suffocation incidence of the newborn was about 606％,and the incidence of complication and sequelae of the puerperaants was about 5.58％.Conclusion: The perineotomy parturitono may have remarkable significance in the second course of parturition in avoiding perineum laceration,alleviating the pressure of the pelvic tissues on fetus' head,shortening the second course of parturition,as well as in decreasing the suffocation incidence of the newborn.

会阴切开阴道助产术在第二产程中为避免会阴及盆底严重裂伤，减轻盆底组织对胎头的压迫、缩短第二产程、加速分娩、降低新生儿窒息率具有很大的意义，只有合理掌握手术的指征以及避免过多干预和进行手术助产，会阴切开阴道助产术就可取得良好的效果，侧切率就能得到很好的控制，否则将会产生一系列并发症，对母婴带来很大的危害。

objective to study the effects of ethanol extract of the huangqi on the skeletal muscles contractility in rats.methods the gastrocnemius and soleus muscle contractility were observed by stimulating the sciatic nerve with electricity after affusing stomach with ethanol extract of the200mg/kg dosage of huangqi for2weeks.results the ethanol extract of the huangqi could shorten latent period,increase maximal contractive extent and time and relaxative time at the twitch and complete tetanus of gastrocnemius and soleus muscles.conclusion the ethanol extract of the huangqi can enhance skeletal muscles contractility in rats.

方法］ 灌胃给予200mg/kg黄芪酒精提取液2周后，观察大鼠坐骨神经受刺激后腓肠肌及比目鱼肌的收缩力。［结果］黄芪酒精提取液缩短腓肠肌及比目鱼肌单收缩和完全性强直收缩潜伏期，增加单收缩最大收缩幅度，延长最大收缩时间及最大舒张时间；增加完全性强直收缩的最大收缩度，延长最大收缩时间。［结论］黄芪酒精提取液可增强大鼠骨骼肌的收缩力。

On the basis of other scholoar's research, the author makes systematic、complete and throughgoing research on characteristics and predication of strata movement for the first time in this paper. The creative work makes up land space in domestic and abroad. The author's major work are as follows: 1 According Haken's synergetics view, the author researches the non—linear self —organized evolving process of refeeding, hybriding and cooperating features of internal varables of the roof movement for the first time, and makes an important conclusion that it will appear chaos at the critical state.

本文在前人研究的基础之上，初步用非线动力学的观点，对岩层运动的动力学特征及预测方法进行了较全面、系统的研究，主要工作如下： 1 根据Haken的协同论观点，初步研究了顶板运动过程中系统内部变量的反馈、耦合、协作的非线性自组织演化过程，得到了顶板运动在突变的临界状态呈现出弱混沌性，其力学行为难以准确预测的结论，较好地解释了岩层突变灾害预测困难的内在原因，并为选择合理的预报时刻，提供了依据。

For strong convective weather , it classified all historical sample events into 4 weather patterns (like northwest , trough area ,west wind and southwest current), established 4 characteristic fields of 400hPa height of HLAFS , then according to the principle of the pattern match calculate and compare the real-time HLAFS forecast products-2-using the similar method inside the big and small key areas , establish forecast equation , finally gain conclusion .To duststrom weather, the historical samples were sorted into duststrom and severe duststrom types in 5 regions (they are whole area, west, central, middle-west and middle-east of Inner Mongolia ),moreover set up different sample databases about ECMWF fields (including 500hPa height, 850hPatemperature and sea-level pressure).In order to making duststrom forecast at different time level , we first filtered the real-time data by FAX data ,and then used the method of similar range degree to compare the historical data to the actual data of ECMWF .To precipitation weather ,they were divided into 2 types that suit or unsuit airplane artificial precipitation stimulation in line with their emergence time and district .The appropriate weather physical factors come from T106 were chosen to establish the artificial precipitation stimulation prediction model . In the actual application , we can get prediction result as long as use the real-time forecast data of T213 in the prediction model .

针对强对流天气将历史样本天气分为西北气流型、槽区型、西风气流型和西南气流型等4个类型，并建立其HLAFS资料400hPa四种特征场，按照模板匹配的原理，用相似分析方法在大、小两个关键区中对实时HLAFS预报产品进行计算、比较，再建立预报方程，得出结论；对沙尘暴天气将其按区域分为全区、西部、中部、中西部以及中东部5类，再按强弱分别分为强和一般2类，建立ECMWF的3个场(500hPa高度场、850hPa温度场和海平面气压场)的历史资料库，在用传真资料消空之后，用相似离度方法计算实时ECMWF资料，做出不同时次的预报；对降水天气按出现时间和区域分为适合和不适合飞机增雨作业2类，选择T106资料中恰当的气象物理量因子，用BP神经网络算法建立人工增雨降水预报模型，实际应用中将实时T213相关预报资料代入预报模型即可。

The results showed that different topping frequency (at intervals of 15 d, 30 d, 60 d, 90 d) had no evident influence on total wine loss, volatile acid content, T-SO2,F-SO2, wine colour, and wine pH values. Under constant temperature and humidity conditions, total wine loss was closely correlated with the using degree of Chinese barrel and the storage time in Chinese barrel. Volatile acid content slightly rose along with wine storage time. The content of SO2 dropped rapidly during 15 d after wine filling, but dropped tardily ever since. Wine colority rose remarkably during 15 d after wine filling, but climbed tardily ever since. Wine colority change was unstable, it changed wavily and depressed on the whole. In conclusion, it was recommended that the best topping interval was 90 d.

结果表明，橡木桶15 d、30 d、60 d、90 d添桶间隔对红葡萄酒的酒液损失总量、挥发酸含量、T-SO2、F-SO2、颜色、pH值无显著影响；在恒温恒湿的条件下，红葡萄酒的酒液损失总量与橡木桶的新旧程度、桶贮时间密切相关；随桶贮时间延长，葡萄酒中挥发酸略有上升；葡萄酒入桶后15 d内SO2含量急剧下降，以后则缓慢下降；葡萄酒入桶后15 d内色度显著上升，但随后变化平缓，总的趋势是随桶贮时间延长色度缓慢上升；葡萄酒的色调变化不稳定，呈波浪式变化，总的趋势是降低；建议中国橡木桶桶贮红葡萄酒的添桶间隔为90 d。

The content of each part follow as: In the first chapter, as start point and base of the paper, this part focuses on the basic study ofdefinition characteristic of NO, and the existing base of NO-E-Commerceenvironment; In the second one, this part studies the theory base of NO comprehensively applying the theories of core competence competent strategy and transaction cost; Chapter three studies the NO from the coordination of NO, and gives the structure clarification and characteristic of NO firstly, at the same time, put forward the concept of Virtual Enterprise Cluster; Based on such conclusion, studies the model of NO from life cyc organization level process and value chain, and operational mode; In Chapter four, a theoretical explanation was addressed on the above structure by modeling NO with game theory and graphic theory; In the fifth chapter, on the bases of analysis of NO operational risks, coordination mechanism of NO was studied by individually modeling the NO without core and NO with core, and then put forward the solution for coordination mechanism of NO; As an important component of coordination mechanism of NO, Chapter six explored some basic concept of trust and importantly put forward the way of how to build trust in NO, especially investigated the supporting function of valid reputation mechanism of NO for the trust building, importantly an operational method on building reputation mechanism and evaluation method in NO were given; The last chapter applied theconclusion of the paper to investigate the famous trade Web-SUNBU.

全文共分为七章，主要内容如下：第一章作为全文的理论出发点和基础，围绕网络组织的定义、特征以及网络组织生存基础--电子商务环境等方面对网络组织的基本概念进行了阐述；第二章综合运用核心能力、竞争战略和交易费用理论对网络组织产生的理论基础进行阐述；第三章首先从组织协调的角度对网络组织进行了研究，给出了网络组织的结构，分类和特征，同时并给出了虚拟企业群簇；然后在此基础上分别研究了网络组织的生命周期模型、层次模型、过程模型、价值链模型，以及运行模式；第四章综合运用博弈论、图论的相关知识，通过构建网络组织的模型，对上一章所研究的网络组织结构的形成机理给出了一种理论解释；第五章在分析网络组织运行风险的基础上，分别建立无盟主网络组织的博弈论模型和有盟主网络组织的博弈论模型，详细研究了网络组织的协调机制，然后给出了网络组织协调机制的解决方案；第六章作为网络组织协调机制的重要组成部分，本章在讨论了网络组织中建立信任机制的必要性的基础上，研究了网络组织信任关系的类型，提出了在网络组织中如何建立信任机制。

Based on this conclusion, discuss the The quality of cohering between the public art and the place spirt, and and make use of the theories of the place spirit in leading fulfillment, at the same time, to instruct the practice. And put out a tentative plan.Finally, I hope this text can enhance the cognition about humanities and spirit- symbol of public art design.

以此为依据，对老城区的&公共艺术&与场所精神的关系及一致性进行了探讨，并将场所精神的理论运用于对实践的指导，在得出结论的同时，对将来的苏州公共艺术规划和设计提出一个大致的构想。

It puts forward the significance of this paper. It points out that, in the time period of our economic reform, there exist serious social credit crisis and ethic crises in the economic order of society, which have made much negative influence on the development of our socialist economy. Then, the paper deplores into the reasons of these problems and draws a conclusion that at the time when new economic management system is replacing the old one, the confusion of social economic order is normal because of the imperfect new economic management system. The market disorder will necessarily lead to moral landslide and social credit crisis. Thus it will bring about market fluctuation, make worse the environment of system reform, and impede the deepening our reform.

前言提出本文研究的意义，指出我国经济体制转轨时期，在社会经济秩序方面存在着严重的社会信用危机和道德危机，给社会主义市场经济的发展带来严重的不良影响，进而探索出现这些问题的原因，指出由于新旧经济管理体制的更替，新的社会经济管理体制尚未健全，社会经济秩序出现混乱是一种正常的现象，而混乱的市场秩序必然会带来道德滑坡和社会信用危机，使市场出现波动，改革环境恶化，妨碍了改革的进一步深入进行。

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。