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The results showed that the PCR fragment length of root-knot samples was 768bp in cowpea(RKN-1), tomato(RKN-2), balsam pear(RKN-3), Astragalus adsurgens (RKN-19)and rose(RKN-4) from Beijing, peanut fromWeifang (RKN-7) Yantai(RKN-6) and Jiaozhou(RKN-5) in Shandong, tomato from Hexian(RKN-9), cucumber from Suzhou (RKN-8) and Platycodon grandiflorum from Taihe(RKN-11) in Anhui and two kinds of tomato species(RKN-22,23) from China Central Agricultural University; the PCR fragment length of root-knot samples was 769bp in balsam pear from Ganyu(RKN-10) in Jiangsu, tomato from Kunming(RKN-12) in Yunnan, Langfang (RKN-17)in Hebei, Chengdu(RKN-18) in Sichuan and Suzhou(RKN-21) in Anhui and cucumber from Sixian(RKN-20) in Anhui; the PCR fragment length of root-knot samples was 772bp in guava(RKN-13) and pawpaw (RKN-15) from Hainan; the PCR fragment length of the other two samples was both 766bp in tomato(RKN-24,25) from China Central Agricultural University, and the PCR fragment length of root-knot nematodes in cucumber from Hangzhou in Zhejiang(RKN-16) and pepper from Anding (RKN-14)in Hainan was 767bp and 869bp, respectively.

结果表明,北京密云的豇豆、番茄、苦瓜,北京植保站月季,山东胶州,烟台和潍坊的花生,安徽和县的番茄,宿州的黄瓜和太和的桔梗,北京畜牧所沙打旺以及中国农业大学两个番茄品种(RKN-23和RKN-25)上的根结线虫种群的PCR扩增片段长度为768bp;江苏赣榆苦瓜,云南昆明,河北廊坊,安徽宿州和四川成都番茄以及安徽泅县黄瓜上根结线虫种群的PCR扩增片段长度为769bp;海南安定木瓜和石榴上根结线虫种群的PCR扩增长片段度为772bp;中国农业大学另外两个番茄(RKN22和RKN-24)品种上的根结线虫种群的PCR扩增片段长度为766bp;而浙江杭州黄瓜和海南安定胡椒上的根结线虫种群的PCR扩增长度分别为767bp和869bp。

Tall: 64cm # Circumference of Neck: 9.5cm # Circumference of Chest: 25.5cm # Shoulder Width: 13cm # Length of Arm from Shoulder to Wrist: 22cm # Length from Elbow to Wrist: 10cm # Length of Back: 16cm # Circumference of Waist: 20.5cm # Circumference of Hips: 25cm # Length from Hips to Knee: 22cm # Length from Knee to ankle: 15cm # Circumference of Ankle: 7cm # Foot Size: 7.5cm

#高层:64厘米#圆周颈部:9.5厘米#圆周胸围:25.5厘米#肩宽:13厘米#长度从肩至腕臂:22厘米#长度从肘部至手腕:10厘米#返回长度:16厘米#圆周腰围:20.5厘米#圆周臀围:25厘米#长度到膝盖的臀围:22厘米#长度从膝盖到脚踝:15厘米#圆周踝关节:7厘米#脚尺寸:7.5厘米

In plotting scene, the invention connects fiber-optic gyro(1) with full station instrument telescope(3) by adaptor(2), and make optical fibre gyro can follow full station instrument telescope simultaneously do 180 degree turning reel full station instrument cross shaft, respectively on two horizontal positions of east and west direction of gyro axis that differ by 180 degree; each horizontal position process 2 x 2 position gyro data acquisition on full station instrument perpendicular tray that differ by 180 degree; using formula to count out full station instrument collimation axis true azimuth, survey station meridian convergence angle, then can obtain full station instrument collimation axis grid azimuth, realize full station instrument orient.

基于光纤陀螺的全站仪组合定向方法是一种应用于测绘工作的基于光纤陀螺的全站仪组合定向方法,该方法在测绘现场,把光纤陀螺(1)通过连接装置(2)与全站仪望远镜(3)固连,使光纤陀螺(1)可随全站仪望远镜(3)绕全站仪横轴(4)同步做180度转动,分别在陀螺轴东西向相差180度两个水平位置,每个水平位置在全站仪竖盘相差180度的两个竖直位置进行2×2位置陀螺数据采集;利用公式算得全站仪视准轴真方位角、测站子午线收敛角,即可进一步获得全站仪视准轴坐标方位角,实现全站仪定向。

The new developed anti-explosion structural model test devices are used and the integral anti-explosion reinforcement effect comparative simulation tests and study are made on cavities and free type anchor cables, full length grouted one and free type anchor cables as well as the full length type and the half length type anchor cables.

采用新研制的抗爆结构模型试验装置,对毛洞与自由式锚索,全长粘结式与自由式锚索以及全长粘结式锚索与半全长粘结式锚索对洞室的整体抗爆加固效应进行了对比模拟试验研究。

I dance around this empty house Tear us down Throw you out Screaming down the halls Spinning all around and now we fall Pictures framing up the past Your taunting smirk behind the glass This museum full of ash Once a tickle Now a rash This used to be a funhouse But now it's full of evil clowns It's time to start the countdown I'm gonna burn it down down down I'm gonna burn it down 9, 8, 7, 6 5 4, 3, 2, 1, fun Echoes knocking on locked doors All the laughter from before I'd rather live out on the street Than in this haunted memory I've called the movers Called the maids We'll try to exorcise this place Drag my mattress to the yard Crumble tumble house of cards This used to be a funhouse But now it's full of evil clowns It's time to start the countdown I'm gonna burn it down down down This used to be a funhouse But now it's full of evil clowns It's time to start the countdown I'm gonna burn it down down down I'm gonna burn it down 9, 8, 7, 6 5 4, 3, 2, 1, fun Oh, I'm crawling through the darkest home My key don't fit my life no more I'll change the drapes I'll break the plates I'll find a new place Burn this fucker down do do do do dodo do do do do do dodo do do do do do dodo do do do do do dadadada do do do do dodo do (9, 8, 7, 6 5 4, 3, 2, 1) do do do do dodo do do do do do dodo do do do do do dodo doo This used to be a funhouse But now it's full of evil clowns It's time to start the countdown I'm gonna burn it down down down This used to be a funhouse But now it's full of evil clowns It's time to start the countdown I'm gonna burn it down down down I'm gonna burn it down

本人舞蹈解决这个空房子撕裂我们失望你驱逐出去尖叫了大厅纺纱各地,现在我们属于图片框过去您的嘲弄微笑背后的玻璃该博物馆完全灰一旦痒现在皮疹这曾经是一个funhouse 但现在是充满了邪恶的小丑现在是时候开始倒计时我要去烧伤跌下来了我要去刻录下来 9 , 8 , 7 , 6 5 4 , 3 , 2 , 1 ,乐趣回声敲锁车门所有的笑声之前我宁愿住在街道上进行比这个闹鬼记忆我已经称为推动者所谓的女佣我们会尝试傩这个地方拖曳我床垫的码崩溃暴跌众议院卡这曾经是一个funhouse 但现在是充满了邪恶的小丑现在是时候开始倒计时我要去烧伤跌下来了这曾经是一个funhouse 但现在是充满了邪恶的小丑现在是时候开始倒计时我要去烧伤跌下来了我要去刻录下来 9 , 8 , 7 , 6 5 4 , 3 , 2 , 1 ,乐趣噢,我最黑暗的爬行通过主页我的钥匙不适合我的生活没有更多我会改变窗帘我要打破盘子我会找到一个新的地方烧伤这个笨蛋下跌做这样做这样做渡渡鸟做这样做这样做渡渡鸟做这样做这样做渡渡鸟这样做这样做dadadada 这样做这样做渡渡鸟做( 9 , 8 , 7 , 6 5 4 , 3 , 2 , 1 )做这样做这样做渡渡鸟做这样做这样做渡渡鸟这样做这样做渡渡鸟斗这曾经是一个funhouse 但现在是充满了邪恶的小丑现在是时候开始倒计时我要去烧伤跌下来了这曾经是一个funhouse 但现在是充满了邪恶的小丑现在是时候开始倒计时我要去烧伤跌下来了我要去刻录下来

It,s allelchemicals were isolated through extraction, thin-layer chromatography, and column chromatograpathy. After the white clover was infused in ethanol and evaporation, the leavings was dissolved by water. The aqueous extract was extracted by petroleum,chloroform, ethyl acetate and n-Butanol successively, and the bioassay showed that the chloroform phase has obvious inhibitory effect, the inhibitory rate was 44.32%,88.08% on Abutilon theophrasti M., seedling length and root length, 82.08%,92.16% on Echinochloa crusgalli L., seedling length and root length, and the Amaranthus retroflexux L., germination rate is 0, the secondly was ethyl acetate phase.

运用萃取、薄层层析、柱层析等方法对白三叶草地上部分的化感物质进行初步分离,结果表明,白三叶草经乙醇浸提旋转蒸发后用蒸馏水溶解,依次用石油醚、氯仿、乙酸乙酯、正丁醇萃取,生物测定表明氯仿组分对杂草的抑制作用最强,对苘麻芽长和根长抑制率分别为44.32%、88.08%,对稗草芽长和根长的抑制率分别为82.08%、92.16%,反枝苋的发芽率为0;其次是乙酸乙酯相。

The method involves exposing the cells to a compound having the formula I in which: w is a nucleic acid x is a non-amino acid or non-peptide nucleic acid binding group y is a spacer having a chain length equivalent to 1-30 carbon-carbon single covalent bonds or is absent R4 is H or halogen or CH2O-R3; and R1, R2 and R3 are the same or different and are either hydrogen, methyl, ethyl, alkyl, alkenyl, hydroxylated alkyl, hydroxylated alkenyl groups or ether containing alkyl, alkenyl, hydroxylated alkyl or hydroxylated alkenyl groups optionally being an acyl group having a carbon chain length equivalent to 3-24 carbon atoms saturated or unsaturated, with the proviso that at least one of R1, R2 or R3 includes a group having a carbon chain of 3-24 carbon atoms saturated or unsaturated, or to a compound having the formula II in which: w is a nucleic acid x is a non-amino acid or non-peptide nucleic acid binding group y is a space having a chain length equivalent to 1-30 carbon-carbon single covalent bonds or is absent, R5 is alkyl, alkenyl, hydroxylated alkyl, hydroxylated alkenyl group or ether containing alkyl, alkenyl, hydroxylated alkyl or hydroxylated alkenyl group optionally being an acyl group having a carbon chain length equivalent to 3-24 carbon atoms saturated or unsaturated, with the proviso that R5 includes a group having a carbon chain of 3-24 carbon atoms saturated or unsaturated.

该方法包括使细胞暴露于具有式的化合物,在式中:w是核酸,x是非氨基酸或者非肽核酸结合基团,y是具有等于1―30个碳―碳单共价键的链长的间隔基或者不存在,R4是H或者卤素或者CH2O-R3;R1、R2和R3是相同的或者不同的并且是氢、甲基、乙基、烷基、链烯基、羟基化烷基、羟基化链烯基或者包含烷基、链烯基、羟基化烷基或羟基化链烯基的醚,任选地是来源于具有等于3―24个碳原子的碳链长的饱和或不饱和脂肪酸的酰基,其条件是R1、R2或者R3的至少一个包括具有3―24个碳原子的饱和或不饱和碳链的基团,或者使细胞暴露于具有式的化合物,在式中:w是核酸,x是非氨基酸或者非肽核酸结合基团,y是具有等于1―30个碳―碳单共价键的链长的间隔基或者不存在,R5是烷基、链烯基、羟基化烷基、羟基化链烯基或者包含烷基、链烯基、羟基化烷基或羟基化链烯基的醚,任选地是具有等于3―24个碳原子的饱和或不饱和碳链的链长的酰基,其条件是R5包括具有3―24个碳原子的饱和或不饱和碳链的基团。

0Mg/L 6-benzylaminopurine was the optimal concentration of shoots quantity inducing which frequency of inducing shoots and number of shoots per explant were 100% and 11.1 respectively. 0.5mg/L 6-benzylaminopurine was the optimal concentration of shoots length inducing which average length of shoots was 12.0mm. Explant size had significantly effects on direct shoots quantity and length induction. When complete cotyledon used as explant, frequency of inducing shoots, number of shoots per explant and average length of shoots could approach to 100%, 6.7 and 17.1mm respectively. 2.0mg/L silver nitrate could significantly increase shoot quantity induction, both frequency of inducing shoots and number of shoots per explant could be as high as 97.2% and 4.2 respectively. But silver nitrate also could restrain shoots elongation.

结果表明:不同黄瓜品种直接不定芽数量诱导存在显著差异,但长度诱导无显著差异;6-苄氨基嘌呤对直接不定芽数量和长度的诱导作用显著,其中4.0mg/L为数量诱导适宜浓度,其出芽率和每外植体出芽数达到最高,分别为100.0%和11.1,0.5mg/L为长度诱导适宜浓度,其平均芽长12.0mm;外植体的大小对直接不定芽数量和长度均具显著影响,随着子叶的增大诱导直接不定芽的数量和长度增加,其中单片完整子叶为最适宜大小,其出芽率、每外植体出芽数和平均芽长分别达到100%、6.7和17.1mm;硝酸银也对直接不定芽数量诱导影响显著,其中2.0mg/L为适宜浓度,出芽率和每外植体出芽数分别达到97.2%和4.2,但对芽长有抑制作用。

The cypsela of 6 genera (32 species) from eastern Qinghai-Tibetan Plateau were collected. The length, width and length/width of the cypsela were computed, then the relativity about the length, width, length/width of the cypsela and altitude were analyzed.

采集青藏高原东缘6属32种菊科植物的连萼瘦果,测算其长度、宽度和长/宽3项指标,并对上述指标与植物分布海拔高度的相关性进行了分析。

In this research, the ~ and F2 of the crosses between a natural mutant 慪34?with super-minute grain and 慪38?with super-large grain,慡huhui 881擲huhui 527?with middle grain size were carried out to study the inheritance of the grain shape (grain length, grain width, grain thickness and grain length/width ratio) and 1000-grain weight. The main results are summarized as below:? The F1 grain length, grain width, grain thickness and 1000-grain weight of three crosses were lay between the two parents and tended to Y34, which indicated that those grain traits were all governed by the dominant effect of Y34 and influenced by both female and male parents. The differences of F1 grain length, grain width, grain thickness and 1000-grain weight of positive and negative crosses between Y38 and Y34 indicated the existence of cytogene effects.? The broad heritabilities of major grain traits were calculated.

本研究利用一份水稻极小粒自然突变材料Y34与一份水稻极大粒材料Y38、两份常规籽粒大小材料蜀恢881、蜀恢527的杂交F_1及F_2,对主要粒形性状(粒长、粒宽、粒厚、长宽比)及千粒重进行了遗传研究,根据遗传研究的结果利用微卫星标记结合F_2群分法对控制Y34短粒性状基因进行了分子标记定位,主要结果如下:●各组合F_1粒长、粒宽、粒厚和千粒重介于双亲之间且明显偏向于小值亲本 Y34,这表明粒长、粒宽、粒厚和千粒重均主要受小值亲本显性基因的控制并同时也受大值亲本核基因的影响。Y34与Y38正反交F_1在粒长、粒宽、粒厚、千粒重等性状上存在差异,表明存在细胞质效应。

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