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Nowadays, in the low electron dose condition, the information about 3-4〓 can be recorded on the cryoEM micrograph, and the high-resolution reconstruction using the cryoEM has reached 6. 5-8〓 levels. The reconstruction can reveal the protein α helical and recognise β fold secondary structure. Secondly, it is due to the development in 3D reconstruction method, which can be more efficient to determine the orientation and center parameters. The last but not least, it is also owning to the rapid development in computer science and information technology. Such as, some computational task, which previously must be executed by using super-computer, now can be carried out by personal computer, and at the same time, the network speed and band width of data bus and input/output equipment have increased several times. In addition, the improvement of the high quality graphic displaying is available. All these provide strong support for cryoEM high-resolution reconstruction study.

目前，在低电子剂量的情况下，冷冻电镜照片可以记录到3-4 〓的信息，使用冷冻电子显微镜进行高分辨三维重构工作，已经获得6.5-8〓高分辨率水平的结果，可以分辨出蛋白质的α螺旋、识别出蛋白质的β折叠等蛋白质二级结构；其次是，三维重构算法效能的不断提高，使得对二十面体取向和中心参数计算的可靠性大大提高；再次是，由于计算机和信息技术迅猛发展，例如，以往需要使用大型计算机的计算任务已经可以被一般的个人电脑完成，网络速度、数据总线和硬盘等I／O设备的带宽成倍增长，以及图像显示能力的提高都为冷冻电子显微镜高分辨三维重构技术提供了强有力的支持。

After you play for a while you get used to the rhythm of the game but you are most likely to die once in the world one, all the way to die about 6 times for each level in world 9 at end, unlike in the DS version.

当你开始玩一会儿，你会习惯游戏的节奏，但是你极可能在第一世界身亡一次，在最终到达第九世界前身亡六次。不同于DS，Wii有九级世界。

Firsdy, an analysis is made on how left-wing writers defined female ideology in their film making through ideological transformation of "modem" pop culture. Secondly, a conclusion is made that left-wing writers would take the subversion of the existing social system as a final solution to all social problems by exploring why "prostitute" subject prevailed at the then times and analyzing its historical evolving process and revealing the characteristics of prostitute being both exposer and salvation. In addition, the traditional effects on left-wing writers in terms of female are available in this chapter.

首先分析了左翼作家在电影创作中通过对"摩登"的流行文化意义的意识形态改造来完成对女性的意识形态规训过程，之后，通过对"娼妓"题材在本时期流行原因的探索、同类题材的历史演变过程的分析和左翼电影中娼妓作为暴露和拯救双重承受者的身份这一特点的揭示，得出了左翼作家将对现行社会制度的颠覆作为一切社会问题的最终解决方案的结论，同时指出了左翼作家在妇女问题上所受的传统的影响。

Forgive me for having been cold to you. Sorry, I just don't know how to face it. So I pretend to care nothing but in fact I have cheated myself for many times.Yeah, it is heart that can not betray yourself. However I have no choice but to smile all the time, although I feel bitter at the bottom of my heart.

原谅我先前对你的冷漠，对不起，我只是不知如何做好，只是装作一副不在乎的样子，一次次欺骗自己，但是心是不会骗人的，可是我无能为力，原谅我始终微笑，哪怕内心真得很在乎，很痛苦。

Fellini juggles all these conceits with cheerful aplomb, rendering it impossible at times to tell which is which.

费里尼俘获所有这些奇想与开朗沉著，有时因此无法告知哪个是哪个。

Not all researchers were at sea; others trawled through historical records, read whalers' diaries and even studied photos of prize fish taken by Floridian anglers to work out the state of the oceans in earlier times.

不是所有研究人员都在海洋工作，有的人翻阅历史记录，阅读捕鲸人的日记，甚至研究佛罗里达钓客拍的得奖渔获，以此来研究海洋的历史状况。

As soon as Nikolay came in in his full-dress uniform of an officer of hussars, diffusing a fragrance of scent and wine about him, and said himself and heard several times said to him, the words,"Better late than never," people clustered round him. All eyes were turned on him, and he felt at once that he had stepped into a position that just suited him in a provincial town—a position always agreeable, but now after his long privation of such gratifications, intoxicatingly delightful—that of a universal favourite.

身着骠骑兵制服，周身散发出香水和酒的气味的尼古拉，一走进来便说了一句，并且也听到别人对他说了几遍"vautmieusxtardquejamais"，之后便被包围起来；所有的目光都朝向他，使他立即感受到他已进入他在那一省的适当地位——那向来愉快的，如今又在经过长期困苦生活之后陶醉于满足之中的，众人宠爱的地位。

Other times, my friend would simply break even, he neither won nor lost money, but sometimes, Bob would lose his shirt, he would lose all the money he had, he took a beating at a gambling table.

其他时候，我的朋友仅仅收支平衡，他也没有输钱也没有赢钱。但有时会输掉衬衫，他输掉所有的钱。

In these latter times, whenever she dined at Laure's, she donned her diamonds and occasionally brought with her Louise Violaine, Maria Blond and Tatan Nene, all of them ablaze with finery; and while the sordid feast was progressing in the three saloons and the yellow gaslight flared overhead, these four resplendent ladies would demean themselves with a vengeance, for it was their delight to dazzle the little local courtesans and to carry them off when dinner was over.

只有萨丹，每次遇到这些麻烦事时，总是保持冷静，睁着蓝蓝的眼睛，露出处女般的纯洁的面容；她常被两个女人争夺，她被咬，被打，被拉来拉去，而她只说这太可笑了，劝她们最好和解算了。

Yideer's works 《Golden Fairy Tale》was about a forgotten idealizational age which filled with grandiloquence. The golden clothes by himself in his picture was a king of imagery about the old good times that are always inside Yideer. In the pictures, he stand from cloacae; he wander in the air; he lonely stand at higher……In this uphold substance age ,all of belief and ideal like a golden fairy tale. It's far away from us so we can't touch it, but always touch our feeling and hurt us.

伊德尔的《金色童话》，涉及的是一个被人遗忘的豪言壮语的理想化时代，穿一身金色服装的伊德尔本人，就是他心中的一个理想化时代的一种隐喻：他从下水道里钻出来，他游荡在空中，他站在令人生畏的高处……在物欲横流的今天，所有的理想和信仰，像一个金色的童话，离我们遥远而难以触摸，又常常触动我们的神经，让我们隐隐作痛

The concept of equivalent rotationally rigidity is offered and the formula of rotationally rigidity is obtained.

主要做了如下几个方面的工作：对伸臂位于顶部的单层框架—筒体模型进行分析，提出了等效转动约束的概念和转动约束刚度的表达式。

Male cats normally do not need aftercare with the exception of the night after the anesthetic.

男猫通常不需要善后除了晚上的麻醉。