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The diluted monoplast suspension was equally assigned into 3 samples, and separately used for simple modified differential adherence, simple serum-free conditioned medium, and modified differential adherence ＋ serum-free conditioned medium.

①无菌条件取大鼠嗅球组织，消化、离心去除上清液后，用含体积分数为0.2胎牛血清的DMEM/F-12培养基重悬，稀释的单细胞混悬液均分成3份，分别用于单纯改良差速贴壁、单纯无血清条件培养液纯化和改良差速贴壁＋无血清条件培养液纯化的实验。

METHODS: NSCs were isolated from neonatal rats by enzyme digestion and mechanical separation. At the fourth passage, cell clone masses received nestin immunocytochemistry. Remaining cells were dispersed by mechanical separation. Monoclone NSCs were incubated by limiting dilution assay, and made into 108 L-1 monoplast suspension in complete medium. NSCs were assigned into 2 groups. NSCs in the control group were incubated in 10% fetal bovine serum.

酶消化和机械分离法相结合体外分离培养新生鼠神经干细胞，传至第4代的细胞克隆团行巢蛋白免疫细胞化学染色观察，剩余细胞团用机械法分散，采用有限稀释法进行单克隆神经干细胞培养，加入完全培养基制成108 L-1的单细胞悬液，分为2组滴入培养板，对照组加入10%FBS，诱导组加入10%FBS+50 μg/L神经生长因子，培养5~7 d。

METHODS: Olfactory bulb was sterilely collected from rats, digested, and centrifuged. After removal of the supernatant, the olfactory bulb was resuspended with DMEM/F-12 medium containing 0.2 volume fraction of fetal bovine serum. The diluted monoplast suspension was equally assigned into 3 samples, and separately used for simple modified differential adherence, simple serum-free conditioned medium, and modified differential adherence + serum-free conditioned medium.

①无菌条件取大鼠嗅球组织，消化、离心去除上清液后，用含体积分数为0.2胎牛血清的DMEM/F-12培养基重悬，稀释的单细胞混悬液均分成3份，分别用于单纯改良差速贴壁、单纯无血清条件培养液纯化和改良差速贴壁+无血清条件培养液纯化的实验。

It assigned a system engineer exclusively to Ms. Mura until she got up to speed with the computer system.

公司还专门为Mura配了一名系统工程师，在她能够顺畅地使用电脑系统之前来配合她的工作。

A effective inside navar includes the following characteristic: The reliable clerk , duty clear and definite being competent at a job are assigned , appropriate warrant , voucher , precis writer and electronic control etc.

一个有效的内部控制系统包括以下特征：胜任工作的可靠职员，明确的职责分配，适当的授权，凭证、记录和电子控制等。

After catheter insertion under transoral endoscopic guidance, animals were randomly assigned to one of the four groups: acid reflux (pepsin 0.3 mg/mL + taurodeoxycholic acid 0.155 mg/mL + acid pH 2); nonacid reflux (pepsin + taurodeoxycholic acid + pH 6); saline reflux; and unwounded control.

经口腔内镜引导下插入导管，并随机将兔子分组：酸返流组（胃蛋白酶0.3mg/ml＋牛磺脱氧胆酸0.155mg/ml＋酸pH＝2）；非酸返流组（胃蛋白酶＋牛磺脱氧胆酸＋pH＝6）；生理盐返流组；以及无损伤组。

The most surprising result of the SPORT RCT was the high rate of protocol nonadherence (ie, crossover from the assigned treatment group to the other group).

SPORT RCT最为意外的结果便是无法坚持既定方案（即从被分配的治疗组变换到另一治疗组）的比率很高。

To be eligible for the award, a soldier must be assigned or attached to a unit participating in OIF for 30 consecutive days or for 60 nonconsecutive days in Iraq or meet one of the following criteria: 1 Be engaged in actual combat against the enemy and under circumstances involving grave danger of death or serious bodily injury from enemy action, regardless, of time in the Iraq.

要符合获奖资格，士兵必须分配或附加到一个单位，在法语国家组织参加了连续30天或60天或伊拉克的非连续符合下列其中一项条件：1）将在对敌实战从事情况下涉及死亡或严重的身体伤害的敌人行动的严重危险，不管，在伊拉克的时间。

To be eligible for the award, a soldier must be assigned or attached to a unit participating in OEF for 30 consecutive days or for 60 nonconsecutive days in Afghanistan or meet one of the following criteria: 1 Be engaged in actual combat against the enemy and under circumstances involving grave danger of death or serious bodily injury from enemy action, regardless, of time in the Afghanistan.

要符合获奖资格，士兵必须分配或附加到一个单位，参加了在牛津经济连续30天或60天或阿富汗非连续符合下列其中一项条件：1）将在对敌实战从事情况下涉及死亡或严重的身体伤害的敌人行动的严重危险，不管，在阿富汗的时间。

While participating as a regularly assigned air crew member flying sorties into, out of, within, or over Afghanistan in direct support of OEF; each day that one or more sorties are flown in accordance with the criteria shall count as one day towards the 30 consecutive or 60 nonconsecutive day requirement.

在作为定期指派机组人员飞行架次为位成员的，参加范围内，或在阿富汗的持久自由行动的直接支持，每一个或多个架次，均符合标准，按照飞行应算为一天一天走向连续30天或60非连续的要求。

Listen,point and check your answers.

听，指出并且检查你的答案。

Warming needle is one of effective treatment methods for knee arthralgia aggravated by cold,and it is simple,safety,so it should be developed in clinical acupuncture and moxibustion extensively.

但以本院科针灸门诊在2005年1月—2006年6月期间共收治膝痛患者100余例，经过临床的诊断后，其中施以温针治疗的48例，疗效显著，报道如下。1临床资料本组病例48