查询词典 assays

You will be responsible for developing and validating in vitro and cell-based biological assays for the testing of drug activities.

你将负责体外和细胞水平药物活性的生物学检测方法的开发和验证。

A variety of techniques from molecular biology have been used in research laboratories to study the effects of DNA binding small molecules, such as the gel mobility shift assay,~1H NMR, DNA foot-printing assay, and fluorescence-based assays, UV-Vis spectrum and viscometry.

目前有很多方法应用于实验室研究DNA与其他物质的相互作用，比如：凝胶电泳分析，核磁共振，DNA足印分析，荧光分析，紫外可见光谱分析以及黏度分析等。

A variety of techniques from molecular biology have been used in research laboratories to study the effects of DNA binding small molecules, such as the gel mobility shift assay,~1H NMR, electrochemistry analysis, DNA foot-printing assay, and fluorescence-based assays, UV-Vis spectrum and viscometry.

目前有很多方法应用于实验室研究DNA与其他物质的相互作用，比如：凝胶电泳分析，核磁共振，电化学分析、DNA足印分析，荧光分析，紫外可见光谱分析以及黏度分析等。

The assays in vitro demonstrated the cells of passage 7 exhibited multipotential differentiation into osteogenic and adipogenic cells.

第7代细胞在体外可定向诱导分化为成骨细胞和脂肪细胞。

Rather than being selectivelyrendered anergic in vivo, circulating survivin-specific CTLswere highly functional as shown by cytotoxicity and interferongamma enzyme-linked immunospot assays in six of nine patients.Survivin-specific CD107a mobilization by T cells was found infive of five patients.

细胞毒性和γ干扰素酶联免疫印迹测定法显示，9例中有6例的外周血survivin特异性CTLs具有高度的活性，该结果与体内选择性提取时无细胞免疫反应的T细胞不同。5例患儿血标本均可检测到T细胞动员的sruvivin 特异性CD107a。

After treatment of benzamide which is an inhibitor of poly polymerase,β-galactosidase activity assays and PCR analysis were performed to test the deletion of LacZ gene and c-Ha-T24ras gene. The results showed that:(1)LacZ gene was deleted from both A13.4-NIH3T3 cells and B12.7-NIH3T3 cells, but neigher from A13.4-HeLa cells nor B12.7-HeLa cells. So it is possible that there is cell line specificity in gene deletion induced by BA.(2) In A13.4-NIH3T3 cells, LacZ gene and c-Ha-T24ras gene were completely deleted at the same time.(3) The transcription activity of exgenous DNA fragment may have same effect on its sensitivity to the deletion induced by BA.

用聚ADP核糖聚合酶的NAD位点抑制剂苯甲酰胺分别处理四种转化细胞后，检测细胞中整合的外源LacZ基因与c-Ha-T24ras基因的删除情况，结果如下：BA诱导的基因删除可发生于NIH3T3转化细胞系，但不能发生于HeLa转化细胞系；位于同一外源表达载体上的LacZ基因与c-Ha-T24ras基因的删除过程是同步的；外源整合DNA片段的转录强度可能直接影响其被BA诱导删除的敏感性。

Based on the absorbance change of indicators with the concentration of hydrogen ion released from the enzyme-catalyzed reaction, a convenient colorimetry method is established for the assay of acidic phospholipase 〓 and glycogen phosphorylase b Brilliant yellow and bromothymol blue are chosen as indicators for assays of acidic phospholipase 〓 and glycogen phosphorylase b by following the absorbance changes at 495 nm and 615nm respectively The method is simple, sample-saving, sensitive and valid for a wide range of enzyme concentrations.

为了研究糖原磷酸化酶的激活动力学和酸性磷脂酶〓的复性过程，我们根据酶催化反应中释放氢离子浓度的变化引起相应的指示剂的光吸收发生变化的原理，建立了一种简捷的比色法，用于测定酸性磷脂酶〓和糖原磷酸化酶b的活性。选用亮黄和溴麝香草酚兰分别作为酸性磷脂酶〓和糖原磷酸化酶b测活的指示剂，在495 nm和615 nm处检测二者的光吸收值的变化，可以测定酶活。本方法的优点是，可在比较宽的酶浓度范围内进行活力测定，而且操作简单、节省样品、灵敏度高。

PKC and PKA activity assays showed that 0.03μmol/L of 20-HETE increased PKC and PKA activity in isolated cardiomyocytes, while chelerythrine (5μmol/L) and H-89 (5μmol/L) inhibited these effects.

PKC和PKA活性测定的结果表明20-HETE(0.03μmol/L)增加心肌细胞PKC和PKA的活性，并且白屈菜赤碱(5μmol/L)和H-89(5μmol/L)抑制该效应。

OBJECTIVE: To study the feasibility of selecting chemotherapeutic agents in vitro chemosensitivity assays with peripheral blood lymphocytes.

目的：探讨应用肿瘤患者的外周血淋巴细胞进行体外药敏试验指导临床化疗用药的可行性。

The following parameters were used in assays:morphology,chlor ophyll a and β-carotene.

通过测定藻丝形态参量、叶绿素a、β胡萝卜素，研究半导体激光对藻生长的影响。

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。