查询词典 assays

Here, using PCR-assays, we have studied on the tissue distribution of S-symbionts in colonized species of G. m. morsitans, G. p. palpalis, G. austeni and G. brevipalpis, and found that S-symbionts were primarily detected in the midgut, hemolymph, milk gland of every species and also existed in older fly's muscle, testes and fat body.

在本研究中，我们利用PCR检测技术分析了S-共生菌在实验室繁育的G.m.morsitans，G.p.palpalis，G.austeni和G.brevipalpis等采采蝇物种或亚种中的组织分布状况，发现S-共生菌均主要存在于每一物种的中肠、血淋巴及乳腺中，而在年长的个体中，S-共生菌还存在于肌肉、精巢和脂肪体等其它组织器官。

In vitro binding assays revealed that different NAP1-like proteins bind, with distinct relative binding strengths, to different classes of histones.

体外结合实验显示了不同的NAP1类似蛋白与不同的组蛋白的结合能力存在差异。

LACS substrate specificity assays indicated that the pXT166 preferentially activated long chain fatty acids and saturated fatty acids.

分析该基因编码蛋白的底物偏好性显示，长链饱和脂肪酸是其优先底物。

We employed a cell line of mouse podocytes immortalized with the simian virus 40 large tumor antigen gene, and examined the cell proliferation using colony-forming and MTT assays following treatment with various concentrations of bFGF. BMP-7, alpha-smooth muscle actin e podocytes were detected by western blot. Methyl blue staining was used for the observation of cell morphology.

利用SV40肿瘤抗原转染所获得的永生性小鼠足细胞系培养，以集落形成法和噻唑蓝MTT还原实验测定不同浓度bFGF刺激下的足细胞增殖，采用SDS-聚丙烯酰胺凝胶电泳和Western blotting 检测BMP-7、alpha-平滑肌肌动蛋白、以及波形蛋白的蛋白质水平表达，同时以甲基兰染色观察细胞形态。

By thismethod, the erythromycin titres of biological and are chemical assays 11.1% and 24% higher than that of fixed temperature (32℃) fermentation.

采用此变温培养进行发酵，红霉素的化学效价和生物效价比恒温32℃培养对照组分别提高了24%和11.1%。

Genotype determination in patients co-infected with HCV and HIV should be performed by methods relying on sequence analysis, since serotyping assays have shown a lower sensitivity in co-infected cohorts. HCV-RNA concentration is a strong predictor of response to therapy.

基因型决定了合并感染HIV患者应该接受何种治疗策略依赖于对HCV-RNA的序列分析，只从血清型分析发现对于合并感染患者而言敏感性太低以来，HCV-RNA滴度测定是治疗应答的一个最强预测因子。

Nematode assays showed that the resistance to root-knot nematodes was significantly improved in some transgenic lines compared to untransformed susceptible control, and the resistance was inheritable in their selfed progenies.

目前国际上已从番茄中发现了8个抗根结线虫基因，其中3个抗性基因Mi-1、Mi-3和Mi-5在番茄染色体上的定位已经清楚，这篇文章没有明确是Mi基因家族的哪一种？

Reporter gene assays showed that ZNF325 and ANKZF1b both are transcriptional repressors because overexpression of ZNF325 and ANKZF1b in the COS-7 cells inhibit the transcriptional activities of AP-1 and SRE.

ZNF325转录活性的拆片分析和RNAi分析进一步证明C2H2锌指区域对报告基因AP-1和SRE起着重要的抑制作用。

Null mutant Δmnh6 produced appeared offwhite hyphae, showed reduction in growth, conidiation and appressorium formation, and also showed reduction in appressorium turgor pressure. And the hyphae were more readily wettable by a solution containing SDS and EDTA and more easily digested by fungal cell-wall-digesting enzymes. The appressoria produced by Δmnh6 mutants showed diminished host penetration and the infectious hyphae of Δmnh6 mutants showed reduced in planta growth. Targeted deletion of mnh6 resulted in the greatly reduced virulence capacity to barley and rice CO39 in infection assays.

突变子Δmnh6的菌落形态不同于野生型GUY11，菌落呈灰白色，菌丝稀疏，生长速度变慢，产孢菌丝细胞壁的溶剂疏水性消失，菌丝细胞壁容易被细胞壁降解酶降解，产孢减少，孢子形态变得细长，孢子的附着胞形成率降低，附着胞的膨压降低，附着胞对寄主植物表面的穿透能力减弱，侵入后生长速度减慢，喷雾接种后对大麦和水稻CO39的致病性消失。mnh6基因重新引入Δmnh6后，突变子的表型恢复。

Biological assays of the toxin showed that it could inhibit rice growth, cause rice seedlings to wilt and make tobacco cells necrosis.

毒素具有抑制水稻生根、使水稻秧苗萎蔫和对烟草细胞坏死的作用。

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。