查询词典 assays

The immunoreaetivities of the prepared antisera with recombinant and native PrP proteins were evaluated by ELISA and Western Blot assays.

ELISA和Western Blot检测所制备抗体与重组和天然的PrP蛋白的免疫反应性。

Methods Indirect immnofluorescence assay was applied to identify specificity of the two groups of monoclonal antibodies prepared with crude antigen and recombinant antigen of Aspergillus fumigatus,respectively.Two different double monoclonal antibody sandwich ELISA assays established with the two groups of antibodies were performed to detect antigents in the cell culture supermatants of 19 common species of Aspergillus,Penicillium marneffei,and 5 species of Candidas.

采用天然烟曲霉抗原免疫，获得广谱针对曲霉抗原的单克隆抗体；采用重组烟曲霉抗原获得特异性针对烟曲霉抗原的单克隆抗体，用间接免疫荧光鉴定，并分别建立2种双抗体夹心ELISA法，对19种常见的环境和临床分离曲霉株、马尔尼菲氏青霉菌及念珠菌培养液进行检测。

Moreover, the result about the inhibitory effect on ryanodine binding of concentrated chelators indicates that using proper concentrations of chelators is essential in ryanodine binding assays.

此外，高浓度螯合剂抑制ryanodine结合的结果还表明，在ryanodine结合实验中，使用的螯合剂浓度不能过高。

Replating of cells from primary LTC with different feeders into secondary LTC-IC assays containing the best combination of engineered feeders showed that LTC-IC maintenance could be significantly enhanced (up to 7-fold as compared with primary cocultures containing HMF).

Replating细胞从小学中校不同馈线到中学中校集成电路检测含有最佳结合工程馈线表明，长期培养集成电路维修可以大大提高（最高可达7倍相对于小学cocultures含有羟甲基糠醛）。

Crofts N%Maskill W%Gusst ID Evaluation of enzyme-linked immunosorbent assays:a method of data analysis Journal of Virological Methods , 1988,1

李秀华%许四宏%宋爱京%王佑春 HIV抗体诊断试剂特异性样品库的建立临床输血与检验， 2006，4

The effect of aluminum ions on the Fe2+-initiated lipid peroxidation in liposomes was studied by chemiluminescence, TBARS, conjugate dienes assays and ESR spectroscopy.

利用化学发光、TBA 反应与测量共轭二烯的方法观测了Al3＋对Fe2＋启动的卵磷脂脂质体脂质过氧化的影响。

The IWFs were purified by (NH4)2SO4 precipitation fractionation, and then applied to Sephadex G-75 column. The column eluates were collected separately according to A280 nm and injected into the health wheat leaves to examine the eliciting activities by PAL, PO assays.

IWF经硫酸铵粗沉淀后，将蛋白沉淀用重蒸水重新溶解后进行凝胶过滤柱层析，以280nm紫外吸收检测、收集不同的蛋白峰，分别注射到健康的Thatcher叶片中，以苯丙氨酸解氨酶(phenylalanine ammonialyase，PAL)、过氧化物酶(peroxidase，PO)防卫反应作为评价诱导活性的指标，检测活性成分在各部分中的分布和诱导活性的强弱。

Now, there is still no suitable functional assays for DCV exocytosis in C. elegans.

但仍然缺乏在线虫上对DCVs分泌进行功能分析的合适手段。

The results of pharmacological assays, together with the structural characterization of the derivatives, indicate that immunomodulatory activity of glucan derivatives not only depends on the glycosyl residue linkages and sugar chain conformation, but also is closely related to the nature of functional groups, degree of substitution and its distribution in a glycosyl residue and along the sugar chain.

免疫药理分析表明，不同结构和电荷特性的取代基的引入可以极大地改变原多糖的免疫调节活性，不同取代度的同一种取代基也可能具有完全不同的免疫调节效应。

In the present study, patch-clamp whole-cell recording, transwell migration assays and organotypic cerebellar slice cultures were used to examine the effect of melatonin on granule cell migration.

在这篇研究中，我们使用膜片钳、Transwell迁移分析法和小脑脑片培养方法，观察MT对颗粒细胞迁移的影响。

Neither the killing of Mr Zarqawi nor any breakthrough on the political front will stop the insurgency and the fratricidal murders in their tracks.

在对危险的南部地区访问时，他斥责什叶派民兵领导人对中央集权的挑衅行为。

In fact,I've got him on the satellite mobile right now.

实际上 我们已接通卫星可视电话了