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Different concentration of NAA and PP333 were sprayed on the stem and leaves of Jasminum sumbac L. at early florescence stage, the effects on Jasminum sambac L. floral bud formation were studied. The physiological and biochemical parameters such as chlorophyll, soluble sucrose and starch content,α-amylase activity, were used in the assays.

在茉莉开花前期分别使用不同浓NAA与PP333均匀喷施于植株茎、叶片等生长部位，研究其对茉莉开花的影响，分析测定茉莉新梢叶绿素、可溶性总糖、淀粉含量与α-淀粉酶活性等生理生化指标。

2Enzyme-linked immunosorbent assays was used for the level of serum testosterone, and inmmunohistochemistry was used for the condition of expression of proliferating cell nuclear antigen, with the karyon in orange brawn yellow being taken as positive.

2血清睾酮水平酶联免疫吸附法检测。增殖细胞核抗原的表达情况采用免疫组化法检测，以细胞核呈桔黄色或棕黄色颗粒为阳性。

High proportion of cells was in Sub-G1 phase of cell cycle and the cells could not grow in soft agar to form colonies. On the contrary, the three breast cancer cell lines, Bcap-37, MCF-7 and MDA-MB-231, are anoikis resistant, as indicated by DNA laddering and FCM assays.

而Bcap-37、MCF-7、MDA-MB-231三种乳腺癌细胞则具有抗脱落凋亡的特性，分别与它们各自贴壁培养的细胞比较，胞浆DNA琼脂糖凝胶电泳表现无明显差异，FCM所检测到的凋亡峰也无明显区别。

Obeviously this strategy is advantageously more flexibility because multiple discrete assays can be carried out simultaneously in mixing microvolume multi-samples.

该技术具有显著的灵活性和优点，甚至可以实现多种不同的探针进行同步的微量混合筛选。

In addition of cytomorphologic identification, immunolhistochemic technique of bone morphogenic protein-monoclonal antibody, alkaline Phosphatase staining and concentration assays, as well as osteoblasts reaction to PTH-cAMP were used, according to the biologic character of osteoblasts.

除了在细胞形态学上对其鉴定外，还根据成骨细胞的生物学特性，首次引用了骨形成蛋白单克隆抗体免疫细胞化学技术，以及碱性磷酸酶染色和含量测定，成骨细胞对甲状旁腺激素-cAMP反应等方法，验证了经酶消化分离培养的兔颅骨细胞具有成骨细胞的功能和特性。

The sensitivity rate of different biochemical assays for diagnosis of primary hyper-parathyroidism were: 100% in serum total, free calcium and osteocalcin level, PTH MM (96.4%), intact PTH (92.9%), Chloride/phosphorus ratio (89.3%), nephrogenous cyclic AMP (42.9%), 24h urinary calcium excretion (42.9%), calcitonin (21.4%), phos-phorus (17.9%), C terminal PTH (14.3%) and plasma cyclic AMP (0%).

在不同测量副甲状腺功能之生化检查当中，以血清总钙量、血清游离钙和Osteocalcin之敏感度100％最高，其次依序为PTH MM 96.4％，In-tact PTH 92.9％，24小时尿钙排泄量42.9％，抑钙素21.4％，血清磷17.9％，C-teminal PTH 14.3％，plasma cyclic AMP 0％。

Those assays were used to measure DNA damage ofindividuals exposed occupationally to arsenic.

本研究采用这两个实验对职业砷接触人群DNA损伤进行检测。

ELISA and immunofluorescence assays showed that ER had strong affinity for A431 and A549 cells that overexpress EGFR.

ELISA检测及细胞免疫荧光显示融合蛋白与纯EGFR抗原的相对亲和力为8.0×10~(-9)M，对EGFR高表达的肿瘤细胞的亲和力明显高于低表达的正常细胞。

objective to study the efficacy of the tr/patoc 1 slide agglutination assay for vaccination new pentavalent whole cell vaccine for leptospirosis.methods using tr/patoc 1 slide agglutination assay to detect the searial of serum specimens of 100 healthy people after vaccination for leptospirosis and comparing with the microsopic agglutination test.results both basic immunizaton and 20 days after the enhanced immunization,their positive seroconversion rates were higher than 90% in tr/patoc 1 slide agglutination assay,they were not signficantly different between slide agglutination assay and mat,the antibody titer was greatly lower in 90 days after basic immunization.conclusion the tr/patoc 1 slide agglutionation assay was one of useful assays in evaluation the lately immune efficency of leptospira vaccination.

目的 采用tr/patoc 1玻片凝集试验评价钩端螺旋体疫苗免疫效果及应有价值。方法采用tr/patoc 1玻片凝集试验检测100名健康人钩端螺旋体疫苗免疫后的血清抗体变化，并与显微镜凝集试验比较。结果无论是基础免疫还是加强免疫20？d后，玻凝试验抗体阳性率高达90%以上，与标准的显微镜凝集试验比较差异无统计学意义，玻片凝集试验检测的抗体持续时间较短，90？d后阳性率已大幅度降低，表明sat检测的抗体是早期抗体。结论 tr/patoc 1玻片凝集试验操作简易，可以作为基层卫生机构监测钩端螺旋体疫苗近期免疫效果的方法之一。

The tissues of choice for most organophosphate residue assays are liver and kidney, but perirenal or dorsal fat should be included if the application of a systemic compound is suspected.

大多数有机磷酸酯残留化验的最佳组织是肝脏和肾，但如果怀疑应用了内吸收的化合物，应该把肾周围脂肪或背部脂肪也包括进去。

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。