查询词典 assays

Additionally,this study developed three species-spcific real-time fluorescence quantitative PCR-based assays for rapidly, specificity, sensitivity,detecting Brucella abortus, Brucella melitensis, Brucella suis.

在建立布鲁氏菌属荧光定量PCR检测方法的基础上，根据布鲁氏菌基因组多拷贝插入元件IS711及其下游多态位点，设计了检测牛、羊、猪种布鲁氏菌的引物和牛、羊、猪种特异性荧光探针，优化了牛、羊、猪种布鲁氏菌荧光定量PCR反应体系和条件，建立了牛、羊、猪种布鲁氏菌荧光定量PCR检测方法，组装了牛、羊、猪种布鲁氏菌检测试剂盒，该方法能特异检测上述3个种的布鲁氏菌。

The enzyme assays indicated that Pseudomonas sp. ND6 NahG exhibits broad substrate specificities and metabolizes salicylate, acetylsalicylate, sulfosalicylate, 3-methylsalicylate, 5-methylsalicylate and 5 chlorosalicylate.

酶学实验表明，ND6菌株的水杨酸羟化酶具有广泛的底物特异性，它不仅能代谢水杨酸，还能代谢多种水杨酸的衍生物，如乙酰水杨酸、黄基水杨酸、3-甲基水杨酸、5-甲基水杨酸和5-氯水杨酸等。

These assays can be used to predict tumor aggressiveness.

这一系列研究将被用于预测肿瘤的血管生成情况。

Compounds 1 and 2 were new xanthones, and compound 3 was isolated as a natural product for the first time, and compounds 4 and 6 were isolated for the first time from this genus. The antioxidant activities of all compounds were evaluated by ABTS,FRAP and DPPH assays respectively. Compound 9 showed significant activity by the ABTS and FRAP assays. Compound 1 showed significant activity with IC50 value of 0.31 mg·L-1 in DPPH assay.

化合物1，2为新化合物，化合物3为新天然产物，化合物4，6为首次从该属中获得；化合物9在ABTS和FRAP模型中显示出显著的抗氧化活性；化合物1清除DPPH自由基能力的IC50为0.31 mg·L-1。

To explore the functions of SPANXA1 in cancerous phenotypes CL1-5 cells were transfected with a SPANXA1 expressing vector and evaluated by cell proliferation, cell migration, Matrigel invasion and colony formation assays in vitro as well as mouse metastasis assays in vivo. The results indicated that the induction of SPANXA1 could reduce cell invasiveness. In the other hand, immunostaining showed that SPANXA1 was predominately located at the nucleoplasm.

经RT-PCR验证得知SPANXA1在CL1-0的表现量比CL1-5多100倍以上，於是我们将SPANXA1转染在CL1-5细胞株中，并测量活体外的细胞生长速度试验、细胞迁徙实验、Matrigel侵袭实验、癌细胞群落形成实验及小鼠活体内细胞转移能力，探讨SPANXA1对癌细胞性状的影响，结果发现增加SPANXA1会降低癌细胞的转移能力，在另一方面，我们利用免疫萤光染色法侦测出SPANXA1是存在於核质中，并以西方点墨法再次证实SPANXA1是存在於核质中。

METHODS: CNE-2 human nasopharyngeal carcinoma cells were exposed repeatedly to γ-rays and then screened for a new radioresistant subline named CNE-2R. Double times of CNE-2 and CNE-2R cells were determined by MTT assays. The relative radiosensitivities of tumor cells were assessed by standard colony formation assays. Survival rates after exposed to irradiation were measured by MTT assays. The time courses of cell cycle distribution before and after irradiation were investigated by flow cytometry.

用γ射线反复照射CNE-2细胞，筛选出放射抗拒细胞株CNE-2R；检测细胞生长的倍增时间；应用细胞克隆形成实验检测细胞的放射敏感性；MTT法检测照射后不同时间的存活分数；用流式细胞术检测细胞的周期分布特征。

Primarily, the thesis assays the condition of network framework, the network was divided into three levels in terms of network management in order to establish the distributed method ,and still pointing out the intention and denotation of studying the Topology Discovery.Next, the thesis analyzes SNMP protocol minutely, including its development, principle, and the Network Management system that established on the SNMP protocol. Have analysed MIB in detail and the application way of MIB in network management .The thesis also assays the ICMP protocol, describing its working principle and the format of datagram minutely , and describing two important tools of ICMP- Ping and TraceRoute in detail. On the base of upper analysis, the thesis expounds a kind of distributed Topology Discovery project, and book it in one autonomy system. Topology Discovery was divided into two levels in terms of network management, and analyses the way of linking up between the two level. The thesis minutely assays Router-Level Topology Discovery technology basing on SNMP and Subnet-Level Topology Discovery technology basing on ARP and ICMP. According to these analyses, the thesis explores the specific methodology and the technology of XML data object which using the WinSNMP API to achieve topology discovering system on the development platform of Visual C++, and also analyses the technology of topology analysis and topology graph minutely. Moreover, the thesis assays the technology of basic firewall and Topology Discovery response strategy. Finally, the thesis analyses the underlying blind problem of the topology finding, and also analyses the reason that the blind problem produced and the way that reduced. The distributed algorithm that this paper puts forward has a certain directive significance in wireless network or other fields.

本文首先分析了网络结构状况，将网络从网络管理的角度划分为三个层次，为分布式的方法奠定了基础，同时还指出拓扑发现研究的目的及意义；接着本文分析了SNMP协议，详细分析了SNMP协议的发展状况，协议的工作原理，以及由SNMP协议基础上建立的SNMP网络管理体系，详细分析了MIB，以及MIB在网络管理上的应用方式；本文又分析了ICMP协议，详细描述了ICMP的工作原理和数据报格式，并详细描述了ICMP的两个重要工具-Ping 和TraceRoute；然后本文在结合上述分析的基础上，提出了一种分布式的拓扑发现方案，将拓扑发现拟订在一个自治系统内，将拓扑发现从网络管理角度划分为路由器级和子网级两个层次，分析了两个层次之间的衔接方式，同时从拓扑地域的角度将拓扑发现过程分布化，分析了分布式算法的具体方法和分布式结点之间的数据通讯方法，本文详细分析了基于SNMP的路由器级拓扑发现技术和基于ARP和ICMP的子网级拓扑发现技术；根据这些分析，本文利用XML数据对象作为分布式算法中的数据对象，分析了XML的技术，本文使用Visual C++开发平台实现网络拓扑发现系统，详细分析了使用WinSNMP API实现基于SNMP的路由器级拓扑发现和基于ARP的子网级拓扑发现，分析了使用Winsock编程实现基于ICMP的子网级拓扑发现，本文还对拓扑分析和拓扑图的绘制技术作了较细致的分析；本文最后还分析了基本防火墙技术，分析了几种类型的防火墙对拓扑发现带来的影响，以及在拓扑发现时的应对策略，本文还分析了拓扑发现中可能产生的盲点问题，分析了盲点产生的原因以及拓扑发现中减少盲点的方法。

Establishing the specific phenotypic variant of methylmalonic acidemia requires studies on vitamin B12 responsiveness, C14 propionate tracer assays, complementation analysis, and cobalamin distribution assays.

要了解甲基丙二酸血症特异表型的类型需要进行维生素B12敏感性测试、C14丙酸盐示踪测试、互补分析和钴维生素分布分析。

Results suggested that Terminalia catappa leaves have no effect on cell viability of SCC-4 cells. The modified Boyden chamber assays revealed that a treatment of Terminalia catappa leaves significantly inhibited the cell motility/invasion capacities of SCC-4 cells.

在本实验中，利用 50%酒精萃取的榄仁树的叶之萃取物处理 SCC-4 细胞株，并利用 MTT assays 分析方法显示榄仁树的叶萃取物并不会影响 SCC-4 细胞的存活率，而当癌细胞转移时常常会伴随著细胞外基质的分解及细胞移动能力的改变，接著我们利用 modified Boyden chamber assay 发现榄仁树的叶萃取物具有抑制 SCC-4 细胞的移动与侵袭能力。

objective to establish immunological methods specific for detecting antigens in different groups of monoclonal antibodies.methods indirect immnofluorescence assay was applied to identify specificity of the two groups of monoclonal antibodies prepared with crude antigen and recombinant antigen of aspergillus fumigatus,respectively.two different double monoclonal antibody sandwich elisa assays established with the two groups of antibodies were performed to detect antigents in the cell culture supermatants of 19 common species of aspergillus,penicillium marneffei,and 5 species of candidas.results the results of indirect immnofluorescence assay indicated that the monoclonal antibodies prepared with crude antigen of aspergillus fumigatus were specific for antigens in both clinical isolates and environmental isolates of aspergillus, whereas the other group of monoclonal antibodies was proved to be specific for aspergillus fumigatus of both clinical and environmental isolates.the elisa assay established with the crude antigen-specfic monoclonal antibodies could detect both of the clinical and environmental isolates of aspergllius, while the other assay could only detect aspergillus fumigatus of both clinical and environmental isolates.and no cross reaction with the cell culture of penialllium marneffei and candidas was observed with the two methods.conclusion the elisa assays can detect both of the clinical and environmental isolates of aspergillus,and differentiate aspergillus fumigatus from other species of aspergillus.

目的 用2组曲霉单克隆抗体建立特异性识别不同种类曲霉抗原的检测方法。方法采用天然烟曲霉抗原免疫，获得广谱针对曲霉抗原的单克隆抗体；采用重组烟曲霉抗原获得特异性针对烟曲霉抗原的单克隆抗体，用间接免疫荧光鉴定，并分别建立2种双抗体夹心elisa法，对19种常见的环境和临床分离曲霉株、马尔尼菲氏青霉菌及念珠菌培养液进行检测。结果间接免疫荧光显示，用天然烟曲霉抗原免疫获得的单克隆抗体(mabs-1)可广谱识别多种曲霉分离株，而重组烟曲霉抗原获得的单克降抗体(mabs-2)仅能特异性结合临床和环境分离的烟曲霉抗原。用mabs-1建立的双抗体夹心elisa法可检测19种常见曲霉株培养液；用特异性针对烟曲霉抗原单克降抗体(mabs-2)建立的双抗体夹心elisa法可特异性检测临床和环境分离株烟曲霉培养液；与其他曲霉株无交叉反应；2种双抗体夹心elisa法与马尔尼菲氏青霉菌及念珠菌培养液均无交叉反应。

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。