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METHODS: The expression level of HIF-1α and VEGF protein and mRNA were also assayed by western blotting and semiquantitative RT-PCR.

半定量RT-PCR检测HIF-1α、VEGF mRNA的表达，蛋白质印迹法检测HIF-1α和VEGF蛋白的表达。

Hematopoietic stemprogenitor cells were assayed by flow cytometry and semisolid culture.

流式细胞术和半固体培养进行造血干/祖细胞特性检测。

The cell proliferation and differentiation were measured as index of stimulating osteoblastic bone formation. Cell proliferation was assayed by MTT method with fluoride sodium as positive control.

以成骨样细胞的增殖和分化为活性检测指标，成骨样细胞的增殖以MTT法测定，氟化钠为阳性对照药。

SPANXA1 (sperm protein associated with the nucleus, X-linked, family member A1) is one out of approximately 300 candidate genes. The expression level of SPANXA1 is up to 100-fold in CL1-0 compared with that of CL1-5 assayed by RT-PCR.

SPANXA1（sperm protein associated with the nucleus， X-linked， family member A1）即为其中之一，因为此基因存在於X-染色体上，在遗传及演化上有著重要意义，且与肺癌的相关性尚未有任何的文献报告，值得我们更进一步的探讨。

The model animal was medicated with tonifying kidney plus strengthening vital energy for 6 weeks, then, the production of IL 1 by peritoneal macrophages, IL 2 and IL 12 by splenetic lymphocytes were assayed with the methods of biological activ...

结果 模型动物IL 1、IL 2及IL 12的活性水平较正常小鼠下降，其mRNA表达均受到抑制，与正常对照组比差异显著(P 0 5 )；经益气补肾方药治疗后，三种细胞因子活性水平及其mRNA表达均有不同程度的提高。

The SFC of palm stearin and soybean oil blend of 40:60 and 30:70 after enzymatic interesterification with packed bed reactor were assayed, which could provide reference for future application research.

采用填充床式反应器对40：60和30：70的棕榈油硬脂和大豆油的混合物进行酶法酯交换反应，测定了反应产物的SFC曲线，为将来的应用开发提供参考数据。

Methods Since Nov 2005,we have assayed 30 couples of sample,that to those patients whom needs X-ray check for their styloid process, taken photos styloid process by used classical Lat viewand by used Orthopantomograph,then study it according to those images.

从05年11月起，我们连续选取了30例需要做茎突X线检查的病人，同时摄取常规茎突侧位影像和用口腔全景机拍摄的茎突影像，然后根据这些影像资料对它们进行对比和分析。

And cell differentiation was assayed by measuring the cellular alkaline phosphatase activity with dexamethasone as positive control. The influence of ethanol and dimethyl sulfoxide on cell proliferation and differentiation assay was also investigated.

成骨样细胞的分化采用测定细胞内碱性磷酸酶活性的方法，地塞米松为阳性对照药，同时考察了溶剂乙醇和二甲基亚砜对细胞增殖和分化的影响。

The concentrations of interleukin(IL-6) and tumor necrosis factor-α in the supernatant were also assayed.

但内毒素抑制大鼠肝细胞白蛋白表达是否通过细胞因子的介导仍不明确。

Gene bcl-2 and bax expression in tissue sections were assayed with immunohistochemistry supersensitive kit with a streptavidin-biotinperoxidase complex method.

应用链霉菌抗生物素蛋白-生物素-过氧化物酶复合物法免疫组化染色超敏试剂盒检测血管壁bcl-2、bax基因表达情况。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。