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Methods:The expressive plasmids of AML1b and AML1-ETO were transfected into CV-1 and 293 cells.The expression level of endogenous pig7 gene was detected by Realtime-PCR.The luciferase reporter plasmids containing pig7 enhancer/corresponding mutant sequences were constructed and co-transfected into CV-1 cells with expressive plasmids of AML1b and AML1-ETO.The transactivity of pig7 enhancer was assayed by luminometer.

研究方法：AML1b和AML7-ETO表达质粒分别转染及共转染CV-1和293细胞，用Realtime-PCR方法检测上述基因对两种细胞内源性pig7基因mRNA表达水平的影响；构建含AML1b结合位点的pig7基因增强子序列以及相对应的突变位点的荧光素酶报告基因质粒；将表达载体/突变载体与AML1b或AML1-ETO基因瞬时共转染CV-1细胞，分析AML1b、AML1-ETO对报告基因的转录调节作用。

Methods 40 healthy adult mice were randomly divided into control group, CTX immune-suppressed group, Fb treated group, Fb combined with CTX treated group. The influences of Fb in NK cell activity were determined by the method of NK mediated cytotoxicity test;the multiplicative capacity of lymphocyte was assayed by lymphocyte transformation rate of T cell,and the mice spleen T lymphoblast multiplication analytical method was used to measure the IL-2 level.

健康成年小鼠40只，随机分为对照组、环磷酰胺免疫抑制组、黄蘑碱溶性多糖活性组分正常小鼠给药组和Fb环磷酰胺伍用给药组，采用NK细胞介导的细胞毒试验测定自然杀伤细胞的活性，淋巴细胞转化试验测定T淋巴细胞增殖功能，鼠脾T淋巴母细胞增殖分析法测定白细胞介素2（IL-2）的活性，观察Fb对各组小鼠免疫指标的影响。

Methods The CSF concentrations of DA,5-HT,5-hydroxyindole acetic acid(5-HIAA) and homovanillic acid were assayed by high-performance liquid chromatography in the patients with viral encephalitis and meningoencephalitis(67 cases),schizophrenia(37 cases) and other diseases(21 cases).

方法对病毒性脑炎和脑膜脑炎患者67例、精神分裂症患者37例和病例对照患者21例采用高效液相色谱法检测其脑脊液中5-HT、DA、5-羟吲哚乙酸(5-HIAA)和高香草酸水平，并进行比较。

Methods The CSF concentrations of DA, 5HT, 5hydroxyindole acetic acid (5HIAA) and homovanillic acid were assayed by highperformance liquid chromatography in the patients with viral encephalitis and meningoencephalitis (67 cases), schizophrenia (37 cases) and other diseases (21 cases).

对病毒性脑炎和脑膜脑炎患者67例、精神分裂症患者37例和病例对照患者21例采用高效液相色谱法检测其脑脊液中5HT、DA、5羟吲哚乙酸（5HIAA）和高香草酸水平，并进行比较。

Methods:Bacteriostatic ring after both using together is assayed at asepsis condition by microbion s well diffusion means with using Sarcina lutea as bacterial suspension and ampicinin and erythromycin as model drug.

采用微生物井式扩散法，在无菌条件下，用藤黄八叠球菌作菌悬液，氨苄西林、红霉素标准品为模型药物，测定两者合用后的抑菌圈。

Cows in the perinatal period were used in the experiment.Through clinical symptom and laboratory examination, they were divided into five groups that were healthy multiparity cows,healthy primiparity cows,cows with fatty liver disease, cows with ketosis and cows with rtention fetal membrance. We experimented blood of antepartum 15,8,1 day and postnatal 1,8,15,22 day,then assayed Thyroid hormone, thyroid-stimulating hormone, Iodine ,Selenium, GSH-Px in blood-serum. At last,we analysed data by statistical way .

本实验以处于围产期的57头奶牛为研究对象，通过实验室检查结合临床症状查出其中健康的经产牛、健康的初产牛及患酮病、患脂肪肝、患胎衣不下的自然病例，在奶牛产前15天，产前8天，产前1天，产后1天，产后8天，产后15天和产后22天分别采颈静脉血，检测血清中甲状腺激素(T4和T3)、促甲状腺激素、碘、硒和谷胱甘肽过氧化物酶的含量、摸索出其动态变化并作了统计分析。

Some serum samples from ducks immunized with attenuated DPV were assayed with the ELISA kit, and the antibodies were detected first on day 6 or day 9 post-immunization in ducks vaccinated subcutaneously, orally or nasally, and still detectable on day 60 PI.

应用该试剂盒对皮下注射、口服和滴鼻免疫DPV弱毒的20日龄樱桃谷鸭血液中抗DPV特异性IgG抗体效价进行了测定，皮下免疫鸭于第6d，口服和滴鼻免疫鸭于第9d可在血清中检测到DPV特异性抗体，且至第60d时依然可检测到高效价抗体。

Methods: One hundred and twenty-six patients with UAP were randomly divided into two groups. The control group was treated with Xueshuantong injection, and the treatment group was treated with propylgallate. The clinical therapeutic effects were observed and the blood parameters such as C reactive protein, interleukin-6(IL-6) and tumor necrosis factor-α were assayed by rate nephelometry and enzyme linked immunoadsorbent assay.

选择126临床确诊为UAP的患者，随机分为血栓通注射液对照组和注射用棓丙酯治疗组，进行临床疗效观察，并分别采用速率散射比浊法及酶联免疫双抗夹心法检测C反应蛋白、白细胞介素-6(IL-6)及肿瘤坏死因子-α水平，观察其治疗前后的变化情况。

Methods Serum amylase and serum lipase were assayed in 54 patients with acute pancreatitis,58 patents with cholelithiasis,47 patients with nephrolith and 100 normal control beings.

方法对54例急性胰腺炎患者、58例胆结石患者、47例肾结石患者和50例健康对照组人员进行血清淀粉酶和脂肪酶测定。

Nighty rats were randomly divided into the groups of control, myocardial infarction and doxycycline, which were given orally 48 hours before and 48 hours after MI with 30mg/Kg per day. Protein and mRNA extraction was done on left ventricular samples containing scar and myocardium together. Samples were assayed from 1 day to 4 weeks post-Mi. The activity of MMPs was measured by zymography and collagen amount by the method of chloramine T, the ratio of II III collagen was assessed by immunohistochemical stain. Protein and mRNA of MMP-2, 9, TIMP-1 were determined by Western-Blot and RT-PCR.

分为对照组、心梗组和治疗组(D组，术前两天至术后两天，口服Doxycycline，30mg／kg／天)，分别于术后第一天、术后一周、术后两周、术后四周取心肌组织，采用免疫组化测定胶原含量和Ⅰ／Ⅲ胶原比例，酶谱法测定心梗后MMP-2，9活性蛋白的表达规律，Western Blotting进一步确定酶谱法中所消化条带蛋白的属性，逆转录-聚合链反应法测定心梗后MMP-2，9和TIMP-1的mRNA的变化规律，运用Acuson Sequoia 512超声心动图机分别测定心梗组、治疗组在两周和四周时左室前壁、后壁厚度以及左心室舒张末内径和左室射血分数。

Neither the killing of Mr Zarqawi nor any breakthrough on the political front will stop the insurgency and the fratricidal murders in their tracks.

在对危险的南部地区访问时，他斥责什叶派民兵领导人对中央集权的挑衅行为。

In fact,I've got him on the satellite mobile right now.

实际上 我们已接通卫星可视电话了