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Activity of GAD was assayed by measuring the formation of GABA.

根据L-谷氨酸脱羧酶能够分解谷氨酸产生γ-氨基丁酸和CO2的特性，通过测定产物γ-氨基丁酸推断酶活。

Methods: Parallel-plate-flow-chamber was used to assay the influence of shear stress on endothelial cells morphology and cytoskeleton and apoptosis and NO secretion. endothelial cells morphology was observed under microscopy, cytoskeleton was showed by Coomassie brilliant blue staining, fluorescence stain, and transmission electron microscopy, apoptosis was detected by FCM, NO of circulating culture medium was assayed by NO kit.

细胞形态显微镜下直接观察；细胞骨架通过考马斯亮蓝染色、荧光标记和透射电镜进行观察；细胞凋亡通过流式细胞仪检测进行分析，一氧化氮通过流动循环培养基的收集后应用检测试剂盒进行检测分析。

In this article, the effect of matrix on the intensity of RRS is analyzed theoretically based on the matrix negative absorption effect; the effect of pH is analyzed based on the conditional stability constant which is assayed by spectrophotometry; and MG and CV are analyzed qualitatively and quantitatively through detecting the intensity of RRS by the fluorescence spectrophotometer synchronously scanning.

本文采用负吸收效应，从理论上对体系基体的作用进行分析；采用紫外-可见分光光度计测定吸收光强度，求出缔合物的条件稳定常数，对体系酸效应进行分析；采用荧光分光光度计测定共振瑞利散射强度，对孔雀石绿和结晶紫进行定性、定量分析。

MethodsⅠMaximal electroshock seizure was adopted to analyse the relative of dose and effect, relative of time and effect, including maximal efficacy and potency of these five extractives in mice given by ig administration.ⅡMetrazol seizure test was used to compare seizure latency, anticonvulsant rate and mortality of mice with every extractives.ⅢPharmacodynamic parameters of five alcoholic extractives were assayed by means of Pharmacological Effective Method.ⅣUsing the model of seizure rats induced by penicillin localized injected in cortex,we studied the effect of extractives on seizure latency, frequency of epileptiform discharge, highest wave of hippocampus EEG(Recoded by RM6240C multichannel physiological signal collection and analysis recorde), the changes of convulsant behaviors and the latency of seizure.

方法①采用最大电休克惊厥模型，分析灌胃小鼠各药的量效和时效关系，比较其效价强度和效能；②采用戊四唑惊厥模型，比较ig小鼠各药的惊厥潜伏期、抗惊厥率和死亡率；③采用药效法，运用数学模式及参数来模拟各药在体内的药效动力学过程；④制备大鼠皮层定位注射青霉素点燃模型，采用RM6240C型多道生理信号采集处理系统记录脑电图，比较ig各药对痫性发作潜伏期及发作程度、痫性放电潜伏期、痫波发放频率及最高波幅的影响；⑤采用HPLC法测定海马区癫痫相关递质的含量，比较各药对Glu、Asp、Gly、GABA的影响。

In order to develop ELISA method for measuring the degradation fragment of fibronectin (MAD2),hybridoma technique was used to obtain the monoclonal antibodyIgG1 against MAD2 without cross-reaction with fibronectin.The sera from 277 patients with hepatocellular carcinoma,76 with metastatic hepatic carcinoma,98 with alimentary canal carcinoma and 156 with chronic liver disease and 48 healthy subjects were assayed with ELISA method using this antibody.

应用杂交瘤技术制备了纤维连接蛋白降解片段MAD2的单克隆抗体；建立了MAD2检测的双抗体夹心ELISA法；对227例肝细胞癌、76例肝转移癌、98例消化道癌、156例慢性肝病患者和48例健康人体血浆MAD2含量进行测定。

MethodsEffect of Epimedium processed by different methods on swelling of the earflaps of mice induced by xylene, croton oil was assayed, degree of swelling was calculated, and compared with saline or indomthacinum.

方法采用二甲苯、巴豆油致小鼠耳壳炎症法测定不同炮制方法的淫羊藿对小鼠耳壳肿胀的影响，pc计算肿胀度，并与生理盐水组、消炎痛组进行比较。

With cells 7404 as the target cells, the killer role of rIL-2 and cytotoxin of LAK cells in the case of pH 6.8, 7.3 and 7.6 will be assayed one by one by the MTT method.

应用细胞计数方法观察pH值分别为6.8、7.3、7.6的培养液，对rIL-2激活单个核细胞的影响。

Meanwhile we also assayed the variation of the Bt protein in the larval body and dejecta. By voidance of dejecta, the level of Bt protein in the larval body was always lower than that in dejecta alter feeding for 12 h, and the difference between both reached the highest at 24 h after feeding.

实验同时测定了幼虫体内及其粪便中Bt毒蛋白含量的变化，结果表明取食转基因水稻叶片12h后，随着大量取食进入体内的毒蛋白随粪便排出，幼虫体内的毒蛋白含量一直低于粪便中的含量，且在24h时两者差异达到最大。

Angiotensin Ⅱ in plasma, heart and kidney was assayed by raio-immunological assay 1 or 18 weeks after denervation.

并采用放免法测定血浆、心脏和肾脏组织的血管紧张素Ⅱ含量。

On this basis a design scheme of the database for SCADA based on the component object model is presented, in which the content and background of IEC 61970 standard is introduced first, then some different component model are assayed , then a design scheme of the database for SCADA based on the component object model is presented, the foreground application of SCADA is connected with the database by components.The new model have some advantages: system have disrelation with develop language ,the peculiarity of COM make the system fit in with different apply program developed with different language;(2) the system can be modified and upgraded easily by means of upgrading the component modules;(3) the system can be enlarged easily by means of adding the new component modules without quiet amendment in old system.

为此，本文介绍了一种基于组件对象模型的SCADA数据库系统设计方案，文中首先介绍了IEC61970标准的内容及产生背景，分析了几种不同的组件模型，在此基础上，提出了一种基于COM组件技术的SCADA数据库设计及访问模式，在此模式下根据SCADA数据库不同的功用划分了不同的组件模块，利用组件连接数据库与SCADA前台应用，基于组件技术的SCADA数据库设计有以下一些特点：(1)与语言无关性，COM的特性决定了此系统上可以应用不同语言开发的应用程序；(2)系统易修改及维护，用户可以通过更新组件模块的方式修改及维护系统；(3)系统易扩展新的功能，用户可以通过开发新的组件方式将新的应用与原系统结合，而不用对原系统做很大的修改。

Neither the killing of Mr Zarqawi nor any breakthrough on the political front will stop the insurgency and the fratricidal murders in their tracks.

在对危险的南部地区访问时，他斥责什叶派民兵领导人对中央集权的挑衅行为。

In fact,I've got him on the satellite mobile right now.

实际上 我们已接通卫星可视电话了