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Methods Blood samples of fifty-two patients who underwe n t open heart surgery with CPB were collected from right internal jugular vein at p oints as pre-CPB, rewarming, aortic declamping, 6 hours and 24 hours after CPB. Cytokines, iNOS, S-100 and NSE were assayed accurately. In addition, its corre lation between them were also analysed.

方法于 CPB前、复温即刻、主动脉开放和 CPB后 6、2 4小时采集 5 2例心脏病患者颈内静脉血，检测 S- 10 0蛋白和 NSE的浓度，并将其与炎性细胞因子白细胞介素- 6 (IL- 6 )、白细胞介素- 8(IL- 8)、肿瘤坏死因子-α和诱导型一氧化氮合成酶的浓度变化进行相关性分析。

For assessing impacts of Bt rice on the non-target insects, the population parameters of the white-backed planthopper, Sogatella furcifera fed on transgenic cry1Ab rice varieties KMD1 and KMD2 and their parent rice variety XS11 were assayed in the laboratory.

通过室内饲养实验及水稻氨基酸和碳、氮含量测定，比较研究了转cry1Ab水稻克螟稻(KMD1和KMD2)与其亲本秀水11对非靶标害虫白背飞虱种群增长的影响。

Methods DNAs from Candida species were amplified by PCR,and the amplicon was assayed by single-strand conformational polymorphism.

以聚合酶链反应对多种念珠菌进行扩增，而后采用单链构象多态性技术对6种念珠菌的扩增产物进行分析。

The seed purity of more than one hundred and thirty samples of six maizevarieties was assayed by using the three testing methods: storage proteins electrophoresis,isozyme isoelectric focusing in acrylamide and examination at field plots. Some importantpoints can be concluded from analyses as spearman order rank correlation on the seedpurity data as follows:First, there were deviations of the real performances to those purities obtained from out or in laboratory testing, even from the examination at field plot that is a routine way. Second, the seed globulin and isozyme electrophoresis, both with characteristics of rapid, cost-effective, devoid of environmental effect and speed, showed consistent purity; while the field-testing gave a higher degree of purity on the high side of cost than that in-lab approaches. Third, a good consistent purity was observed in the field plot and seed globulin testing, whereas inconsistency between field-testing and isozyme process. The last point is that seed globulin electrophoresis be a practical method apt to seed purity testing for maize.

并对130多份样品用三种检验方法(盐溶蛋白电泳、同功酶等电聚焦电泳和田间小区)检测的种子纯度数据作排序相关等统计分析，得到如下结果：室内外检测方法都会与种子真实状况有一定差异，田间种植鉴定与真实情况符合性并不一定最好；盐溶蛋白与同功酶等电聚焦这两种方法检测种子纯度所需时间短，成本低，不受外界环境限制，出结果快，二者的结果没有太大差异；田间种植鉴定成本较高，检测的结果普遍偏高；等电聚焦电泳与田间鉴定的一致性年度间存在差异，而盐溶蛋白凝胶电泳法与田间鉴定的一致性较高；种子盐溶蛋白凝胶电泳法较适于鉴定玉米种子纯度。

The freeze-dried carcasses were then homogenized with liquid nitrogen, and aliquots of dried rat homogenate were assayed for nitrogen and fat concentrations.

被冰冻乾燥的动物体先和液态氮均匀混合，再进行含氮量和脂肪含量检验。

Taken the tuberculin skin test as the reference test,560 serum samples from four local cow herds in the Middle of China were assayed with iELISA.

以结核菌素皮内试验为参考方法，检测了华中地区四个奶牛场的560头中国荷斯坦奶牛和90份进口奶牛结核阴性血清，结果iELISA与TST的总符合率为87.32％（489／560）。

The expression of HO-1 was assayed by immunohistochemistry. In situ terminal deoxynucleotidyl transferase assay was used to observe the numbers of brain apoptosis.

以免疫组化法检测HO-1的表达，原位末端脱氧核苷酸转移酶标记法观察脑细胞凋亡细胞数。

After having been centrifuged, the plasma supernatant was separated and stored at -20℃ until assayed.

在结扎冠状动脉之后的不同时间段也分别取血测量其浓度变化情况。

32P-labeled phospholipid was extracted with chloroform and methanol, and PI3K activity was assayed by scintillation counter.

用PtdIns(4，5)P2，[γ-32P]ATP与重组PI3K p110β催化亚基一起保温的方法测定PI3K的活性；32P标记的磷脂用氯仿和甲醇抽提，加闪烁液于液闪计数仪计数。

Methods the models of epilepsy were made by intraperitoneal injection of pentylenetetrazol and the contents of no,mda and sod of all the epileptic hippocampus were assayed and dealt with all data by statistical analysis.

健康sd大鼠38只，分5组，采用戊四氮腹腔注射制作癫痫模型，测定各组大鼠海马一氧化氮、丙二醛的含量和超氧化物岐化酶的活性。

Neither the killing of Mr Zarqawi nor any breakthrough on the political front will stop the insurgency and the fratricidal murders in their tracks.

在对危险的南部地区访问时，他斥责什叶派民兵领导人对中央集权的挑衅行为。

In fact,I've got him on the satellite mobile right now.

实际上 我们已接通卫星可视电话了