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METHODS:(1) Neonatal rat cardiomyocyte hypertrophic response was assayed by measuring cell surface area and protein content;(2) ERKs activity was determined by Whatman Paper Filter method;(3) Intracellular free calcium concentration (［Ca2+］i) was measured using Fura-2/AM as a fluorescent indicator.

利用培养的新生大鼠心肌细胞，①以蛋白合成速率、蛋白含量及细胞表面积为心肌肥大反应的指标；②用滤纸法测定ERKs活性；③用Fura-2/AM作为钙荧光指示剂测定心肌［Ca2+］i浓度。

Using an anticoagulant it was possible to isolate plasma and intact haemocytes from Helicoverpa armigera larvae. PO and ProPO activities in plasma and haemocyte lysate were assayed when using bovine trypsin as an activator and L-DOPA as a substrate.

用抗凝剂从棉铃虫血淋巴中分离获得了血浆及完整的血细胞，以L-DOPA为底物，牛胰蛋白酶为激活剂，测定了血浆及血细胞裂解液中酚氧化酶及酚氧化酶原的活性。

Six indica and japonica testers were assayed using 70 microsatellite markers.Thirty-six markers distinguishing indicas from japonicas were detected.

应用70个微卫星标记分析了3个籼稻测验种和3个粳稻测验种的多态性，发现其中36个标记可以区分籼粳测验种。

Of curcumins was successfully recovered and 53 wt% purity of curcumins was obtained. The ADH activity in rat hepatocytes treated with these extracts of deoiled turmerics has been assayed in vitro.

实验接著将脱油姜黄粉之萃出物处理在经培养之大鼠肝细胞上并观察细胞释放ADH的活性。

Methods:Serum levels of hyaluronan, laminin, type Ⅲ procollage n and type Ⅳ collagen were assayed in 83 patients with viral hepa titis and liver biopsy was carried out in 62 of them.

病毒性肝炎患者83例，测定其血清透明质酸、层粘连蛋白、Ⅲ型前胶原和Ⅳ型胶原含量，其中62例同时作肝组织病理检查。

Methods We have observed the thyroids of 20 normal controls and 100 patients with thyroid disease, assayed their thyroid function (T 3,T 4,FT 3,FT 4,uTSH), pathological examination, thyroid intake iodine rate, thyroid isotope scan, and these examination were comared.

用彩超观察了 2 0例正常人和 1 0 0例甲状腺疾病患者的甲状腺形态，与其同位素甲状腺扫描进行对比，测定患者的甲状腺功能，13 1I摄碘率，行甲状腺病理检查。

In order to elucidate the regulation mechanism of RU5 region to BFV gene expression, BFV3026 provirus DNA was used to construct the plasmids containing different deletion of R region, which were cotransfected with luc report gene locatied behind the IP promoter to BL12 cells, and luciferase activities was assayed, confirming that U5 region could repress the initiation function of LTR as well as IP. The BFV structure genes with different deletion of R region were cloned closely behind to heterogenous CMV promoter, then transfected to 293T cells, RT activity was performed, testifying the R region was required for BFV pol gene expression, and also the function domain was identified within the 100n.t. sequence at the 5′end.

以牛泡沫病毒(Bovine foamy virus， BFV)中国株BFV3026原病毒DNA为材料，构建R区系列缺失质粒，通过对其转染细胞中RT水平及对缺失质粒与luc报告质粒共转染细胞中萤火虫荧光素酶活性的测定，确立U5区对于BFV3026两类启动子LTR和IP均具有负调控作用；同时将带有不同R区的BFV3026结构基因片段克隆于异源启动子CMV之下，通过对其转染细胞293T中RT酶活性的测定，确立R区对于病毒结构基因pol的表达具有一定的调节作用，并将其功能区域初步界定在R区5′端100bp内。

The BDDcFⅧ gene was ligated behind PUB and 20H1 promotors to create lentiviral vectors pTK161 and pTK162. Meantime lentiviral vectors pTK161' and pTK162' were produced by cloning a green fluorescent protein into pTK151 and pTK152, which was driven by PUB and 2OH1 promotors respectively. Vector supernatant were prepared by using transfer calcium phosphate mediated-cotransfection of 293T cells. The virus vector,△NRF packaging-plasmid, and VSV-G envelope-plasmid was assayed by titers and cFⅧ activity in cell culture supernatant after infection into 293T cells.

用构建携带cFⅧ基因的慢病毒载体pTK161和pTK162（含20HI启动子），同时构建含绿色荧光蛋白的慢病毒载体pTK161'和pTK162'（含2OH1启动子）的方法，分别与包装质粒△NRF、包膜蛋白质粒VSV-G共转染293T包装细胞，将包装好的病毒颗粒再感染293T细胞，检测病毒滴度和培养细胞上清中cFⅧ活性。

Methods:The mRNA content of cytokine-associated genes in placentae was assayed by a complementary DNA microarray representing over 220 cytokine-associated genes.

方法以包含220余种人类细胞因子相关基因 cDNA 片段的基因芯片，检测严格配伍的妊高征和正常胎盘组织中基因表达谱的差异。

Additionally,GS activities and total protein of the ovules and the fibers were assayed.

另外，我们测定了棉纤维发育不同时期胚珠和纤维的GS活力和总蛋白含量。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。