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ASP model was established by injecting with 35 g/L taurocholic acid into the biliopancreatic duct. Both groups were sacrificed at 3, 6 and 12 h with 8 rats at each time point. At scheduled time, bronchoalveolar lavage fluid was drawn and PMNs were then obtained by gradient density gradient centrifugation method. ratio of apoptosis and necrosis of PMN was assayed by flow cytometry and LDH as well as index of lung permeability were measured.

实验组大鼠穿刺胰胆管并注入35 g/L牛磺胆酸钠制作ASP大鼠模型，分别在制模后3， 6， 12 h剖杀，于预定时相取支气管灌洗液，密度梯度离心分离PMN，用流式细胞仪测定PMN凋亡、坏死比例，同时测定乳酸脱氢酶含量、肺通透指数。

ASP model was established by injecting with 35 g/L taurocholic acid into the biliopancreatic duct. Both groups were sacrificed at 3, 6 and 12 h with 8 rats at each time point. At scheduled time, bronchoalveolar lavage fluid was drawn and PMNs were then obtained by gradient density gradient centrifugation method. The ratio of apoptosis and necrosis of PMN was assayed by flow cytometry and LDH as well as index of lung permeability were measured.

实验组大鼠穿刺胰胆管并注入35 g/L牛磺胆酸钠制作ASP大鼠模型，分别在制模后3， 6， 12 h剖杀，于预定时相取支气管灌洗液，密度梯度离心分离PMN，用流式细胞仪测定PMN凋亡、坏死比例，同时测定乳酸脱氢酶含量、肺通透指数。

ASP model was established by injecting with 35 g/L taurocholic acid into the biliopancreatic duct. Both groups were sacrificed at 3, 6 and 12 h with 8 rats at each time point. At scheduled time, bronchoalveolar lavage fluid was drawn and PMNs were then obtained by gradient density gradient centrifugation method. The ratio of apoptosis and necrosis of PMN was assayed by flow cytometry and LDH as well as index of lung permeability were measured.

实验组大鼠穿刺胰胆管并注入35 g/L牛磺胆酸钠制作ASP大鼠模子，分别在制模后3， 6， 12 h剖杀，于预定时相取支气管灌洗液，密度梯度离心疏散PMN，用流式细胞仪测定PMN凋亡、坏死比例，同时测定乳酸脱氢酶含量、肺通透指数。

Primary HMC were cultured and passage 3 and passage 4 of the cells were used. Intracellular calcium release was assayed with confocal analysis. Expression of TGF-β mRNA and the content of supernatant fibronectin were teste...

结果 ：IgA肾病患者aIgA1呈时间依赖性诱导正常HMC细胞内游离Ca2 +释放、TGF -βmRNA表达和Fn分泌，作用趋势与正常人aIgA1相同，高峰时间分别为孵育后 6 0s、2 4 - 36h和36 - 4 8h ，但作用强度显著高于正常人aIgA1；在作用高峰时间，患者组和正常人组Fluo - 3最大相对荧光强度增加值分别为(95 83± 11 4 3)和(5 5 88± 12 72 )，TGF -β/GAPDH的比值分别为 0 96± 0 0 6和 0 74± 0 0 2 ，Fn含量分别为(6 4 5± 0 18)μg/L和 5 5 4± 0 。。。

Six rats of each group were randomly sacrificed on day 7,14,28 and 42 respectively.The histological changes of lung tissue were studied by HE and Masson's trichrome staining.Hydroxyproline level in lung tissue was measured by digestion method.Protein and mRNA expression of transforming growth factor-β1（TGF-β1） were assayed by immunohistochemistry and RT-PCR respectively.

各组分别于造模后第7、14、28及42 d随机处死6只，取肺组织行HE、Masson染色评价肺组织病理变化，消化法测定肺组织羟脯氨酸含量，分别采用RT-PCR和免疫组化法测定肺组织转化生长因子β1（TGF-β1）的mRNA和蛋白表达。

The morphology of tumor cells was observed and the change of quantity was assayed by trypan blue exclusion.

结果在 ACV作用下，GRC- 1细胞的形态及数量无明显改变；GRC- 1/ TK细胞被明显杀伤，数量明显减少，当含 2 5 。0 %GRC- 1/ TK细胞的混合培养细胞即可观察到明显杀伤作用。

When total protein was assayed by 2-D PAGE andstained with sliver, about 2000 protein spots with a pI of pH 3-10 and Mrof 12-120 kDa could be detected. All these protein spots were excised foridentification, among which 213 spots corresponding to 192 differentproteins were identified undoubtful by searching the mass spectra againstthe SwissProt/TrEMBL database.

我们利用双向凝胶电泳对小鼠卵巢蛋白进行了分离，在考马斯亮兰染色后将所有可见的蛋白点切取下，经胰蛋白酶消化以后用MALDI-TOF质谱仪进行鉴定。

Methods:The expression of Fas/Fas-L and apoptosis were assayed by flow cytometry.

用流式细胞仪检测细胞凋亡和Fas、Fas-L的表达。

Methods: The expression of MRP in 37 cases of breast carcinoma were assayed by RT－ PCR.

摘要目的：检测MRP基因在人乳腺癌组织中的表达状况，初步分析其临床意义。

Then the antioxidant effects of these compounds were assayed.

分别测定了各化合物在这些模型中的抗氧化作用。

Neither the killing of Mr Zarqawi nor any breakthrough on the political front will stop the insurgency and the fratricidal murders in their tracks.

在对危险的南部地区访问时，他斥责什叶派民兵领导人对中央集权的挑衅行为。

In fact,I've got him on the satellite mobile right now.

实际上 我们已接通卫星可视电话了