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- 与 assayed 相关的网络例句 [注:此内容来源于网络,仅供参考]
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Lipid profile of serum, plaque area and matrix metalloproteinase content in the innominate artery were assayed. Results: It was shown that the WEP lowered TC, TG and LDL-C levels in serum, and dereased paque area and MMP content in the innominate artery of mice.
结果 蚤休水提液能显著降低小鼠血清的总胆固醇、总甘油三酯以及低密度脂蛋白胆固醇水平,同时减少无名动脉处的斑块面积,降低斑块中基质金属蛋白酶-1(MMP-1)的含量。
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The ethanol extracts of seaweed belonging to Chlorophyta,Rhodophyta and Phaeophyta ,collected from Shandong coast in May 2000,were assayed their activities against two kinds of bacteria of Escherichia coli and Staphylococcus aurens.
分属绿藻门、红藻门、褐藻门的海藻样品于 2 0 0 0年 5月采于山东青岛和威海附近沿海,将海藻样品的乙醇提取物进行薄层层析分离和抗大肠杆菌、金黄色葡萄球菌活性测定。
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After the cell growth curves was recorded, RPE cells of the 3-5th passages were utilized. 2、Three different siRNA (siRNAl,siRNA2,siRNA3) targeting against human cx43 gene and one negative control siRNA were designed and transfected into cultured human RPE cells via liposome reagent. The most effective siRNA can be determined by semi-quantitative reverse transcription PCRRT-PCR. 3、To the most effective siRNA, after transfected into human RPEs with different concentration, the cellular proliferate activities were messured by MTT colorimetry ; the percentages of RPE in different cell circle phase was assayed by FCM; the changes of phenotypical properities were observed with SCM; the protein expression of cx43 was studied through immunocytochemistry stain and Weston blot; the communication intercellular was calculated with FRAP; and the ability of recovery was assessed by using an in vitro wound healing model.4、The total proteins of siRNA1 and RPE were seperated by two-dimensional gel electrophoresis and visualized by silver staining. Proteins with significant expression alterations were selected and their peptide mass fingerprints (PMFs were obtained by matrix-assisted laser desorption/ionization time of flying mass spectrometry (MALDI-TOF-MS).The PMFs were used to search NCBInr database by Auto MS-Fit software.
实验方法:1、培养原代的人RPE细胞,经过细胞角蛋白、S-100和神经胶质原纤维酸性蛋白免疫细胞化学鉴定后,通过AO/PI染色技术确定培养细胞的存活率,描记其生长曲线,第3-5代用于以下细胞实验2、生物合成针对人cx43基因的三条小干扰RNA和一条阴性RNA通过脂质体转染RPE细胞后,通过RT-PCR的方法确定抑制效率最高的干扰片断3、将该片段以不同浓度通过阳离子脂质体转染培养的人RPE细胞后,采用MTT法观察其对细胞的增殖力的作用;通过流式细胞仪观察其对细胞周期的影响;通过扫描电镜观察其对细胞形态的影响;通过免疫细胞化学和Weston blot观察其对cx43蛋白表达的作用;采用激光共聚焦和荧光淬灭恢复技术观察荧光恢复速率平均百分率,评价其对细胞间通讯功能的影响;通过制作RPE细胞损伤模型,观察其对损伤修复能力的作用4、分离纯化转染siRNA的RPE组和正常对照组RPE细胞的全部蛋白质,应用等电聚焦电泳和SDS-PAGE双向电泳技术,银染显示分离出的蛋白质斑点,经凝胶图像分析软件对两个样本进行胶图分析,寻找差异蛋白点。
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Serum and urinary creatinine, urine albumin excretion and NAG were determined photometrically. Urine γ-GT was assayed spectrophotometrically, and urinary B2M was obtained by radioimmunoassay.
每位研究对象均收集24小时尿液样本,测定血清及尿液中肌酐酸浓度、尿中白蛋白排除量、以及尿中 NAG、B2M、γ-GT的活性(以对肌酐酸浓度的比值表示)。
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It caused symptomless infection on garlic, onion, shallot, Welsh onion, Chinese leek, and pea, local lesions on Chenopodium amaranticolor, C. quinoa, and Tetragonia expansa, or systemic necrosis on broad bean, but did not infect leek, narcissus, lily, belldonna lily, tobacco (5 species), Gornphrena globosa, and Physalis floridana in host range test. The virus was propagated in Chinese leek and assayed in C.
其寄主范围包括大蒜、洋葱、葱、韭等葱科作物,但呈无病徵系统性感染,唯不感染韭葱;其他寄主反应包括:红藜、Chenopodium quinoa、及番杏接种叶的局部病斑,豌豆的局部潜隐感染,蚕豆的系统性坏疽等。
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Prestorage and poststorage leukocyte filtration apheresis platelets were transfused respectively to patients with malignancy accompanied by serious thrombocytopenia after chemotherapy. Peripheral blood platelet counts were made at 1h, 24h after platelet transfusion. The effectiveness was assayed by correct count increment. The incidence rates of FNHTR were explored.
46例肿瘤化疗后血小板减少患者,分别输注保存前过滤单采血小板与保存后过滤单采血小板,输注后1h及24h后检测外周血小板计数,以校正血小板计数增殖判定输注效果,并考查FNHTR发生率。
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Animals in those two groups were sacrificed at 24-hour, 72-hour and 7-day after model establishment, six were killed in each group at each time. The tissue of brain was taken out as soon as possible, and the activity of SOD was assayed with auto-oxide of pyrogallic acid.
两组动物分别于造模后24 h,72 h,7 d处死,每次每组处死6只,迅速断头取脑组织,采用邻苯三酚自氧化物测量SOD的活性。
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Methods: The concentrations of scutellarin in plasma and dialysate were assayed by HPLC method.
目的:建立人血浆中野黄芩苷的检测方法,测定野黄芩苷的蛋白质结合率。
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Bronchial asthma in acute episode model was established by a manner of egg albumen sensibilization and provocation. Rats in each group were treated by different drugs. Protein expressions of Fas/FaL-s in lung tissue were assayed by immune histochemistry.
以卵白蛋白致敏、激发的方式建立支气管哮喘急性发作期模型,各组分别给予相应治疗,免疫组化ABC法检测肺组织中Fas/FaL-s的蛋白表达。
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Methods:The syngenic mice were immunized with parental B16 tumor cells and B16.B fusing cells.Then,SPLs of immunized mice were co-cultured in vitro with parental B16 cells,the proliferation of SPLs and the secretion of certain cytokines were assayed.
分别取亲本B16黑色素瘤和融合瘤细胞B16.B主动免疫同系小鼠,观察免疫小鼠脾脏淋巴细胞对亲本瘤细胞体外再刺激的反应能力。
- 推荐网络例句
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The split between the two groups can hardly be papered over.
这两个团体间的分歧难以掩饰。
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This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.
这种做法不仅鼓励了更多的反应,而且减少跟风的可能性。
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The new PS20 solar power tower collected sunlight through mirrors known as "heliostats" to produce steam that is converted into electricity by a turbine in Sanlucar la Mayor, Spain, Wednesday.
聚光:照片上是建在西班牙桑路卡拉马尤城的一座新型PS20塔式太阳能电站。被称为&日光反射装置&的镜子将太阳光反射到主塔,然后用聚集的热量产生蒸汽进而通过涡轮机转化为电力