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In Vitro study on the impact of overexpression of RbAp46 in U937cell lineObjective To study the impact of overexpression of RbAp46 in monoblastic cell line U937. Methods Viability of transfected cells was assayed by trypan blue exclusion.Cell number was counted daily to determine the growth rate.

将处在对数生长期U937／RbAp46和U937／CMV各以2×10~5／ml浓度接种，加入TPA50ng／ml，同时并以培养基作为空白对照，培养72小时后收集细胞，流式细胞术测定细胞表面分化抗原CD11b的表达，RT-PCR分析p21mRNA的表达，流式细胞术检测细胞周期分布情况。

Biological activities were assayed with human peripheral blood mononuclear cells.

以人的外周血单核淋巴细胞测定两重组蛋白的生物活性。

Methods:The DNA content of Mc3 cells is assayed with flow cytometry.The Mc3 cells cycle is analysised with Multicycle software.

流式细胞仪测定细胞DNA含量，Multicycle软件分析细胞周期分布情况。

Methods:Prepared multilaminar liposome vaccine by film evaporaqtion combined with lyophilization; Immunized BALB/c mice with the obtained liposomeencapsulated influenza split vaccine and the routine influenza split vaccine, serum antibodies were assayed on weeks 213 by the haemagglutinationinhibition test and ELISA, the HI test for the special antiHA antigen and the ELISA test for the IgG. Protective immunity against intranasal virus challenge was determined at 12 weeks postvaccination.

采用薄膜法与冷冻干燥法相结合的方法制得多层脂质体；用制得的脂质体流感疫苗和普通疫苗分别免疫小鼠，用血凝抑制实验和酶联免疫吸附试验对免后2～13周的小鼠血清的抗体水平进行检测，HI检测其特异性抗体，ELISA检测IgG抗体水平并对小鼠的保护效率进行检测。

Methods 124 DFSPs were reviewed by light microscopy. 6 cases with areas of myoid/myofibroblastic differentiation were assayed with immunohisto-chemical technique and electron microscopy was applied in two cases.

采用常规HE切片对124例DFSP进行筛选，对6例伴有肌样／肌纤维母细胞性分化的DFSP病例进行免疫组织化学标记，其中2例加做电镜检测。

Distribution and isolation of cellulolytic myxobacteriaWe assayed distribution of and isolated cellulolytic myxobacteria of several environmental types of samples, i.e.

为了更深入地研究和开发溶纤维素粘细菌资源，本文在溶纤维素群粘细菌的分离纯化、生态分布、系统分子分类、降解纤维素机制进行了初步探索。

The association between mineral composition in the pollen of nectariferous plants and the geological background of soil in three mine regions and three orchards was assayed systematically. The results showed that the geological condition of the soil could affect the mineral contents of pollen.

以三矿区和三果园为例，系统地分析了蜜源植物花粉中矿物质成分与土壤地质背景的关系，结果表明：土壤地质背景条件直接影响花粉中矿物质含量。

The proliferation stimulating effects on whole and nonadherent splenocytes of mice were assayed by MTT method; The cell cycles of whole splenocytes were tested with flow cytomytry.

通过MTT法测定小鼠全脾细胞或不粘附细胞的增殖活性，流式细胞仪检测小鼠全脾细胞的细胞周期。

To determine whether F3, similar to other large DNA iruses, encoded proteins that lock or delay apoptosis, mock- and F3-infected FHM cells were osmotically shocked and assayed for DNA fragmentation 3 hours later. NA fragmentation was clearly seen whether or not shocked cells were preiously infected with F3, indicating that infection with F3 did not block apoptosis induced by osmotic shock in FHM cells.

为了证明F3是否象其大DNA病毒一样编码能阻止或延迟凋亡的蛋白，在模拟感染和正常感染的FHM细胞被渗透压休克后3小时检测DNA片段化；结果显示无论是否休克F3感染都能诱导FHM细胞的DNA片段化，渗透压休克并不能阻止细胞凋亡。

NAFLD rat model was established by feeding with high caloric diet. Serum TC, TG, ALT, AST and liver lipid contents, oxidoreduction index ,liver histology were assayed at the end of the 18th week.

以高脂饲料饲养诱发脂肪肝大鼠模型。18周末，处死所有动物，检测血脂、肝功能、肝脂质含量、氧化还原指标、肝组织病理学等。

Neither the killing of Mr Zarqawi nor any breakthrough on the political front will stop the insurgency and the fratricidal murders in their tracks.

在对危险的南部地区访问时，他斥责什叶派民兵领导人对中央集权的挑衅行为。

In fact,I've got him on the satellite mobile right now.

实际上 我们已接通卫星可视电话了