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METHODS Orthogonal design was used as the method of optimizing processing and pinoresinol diglucoside was assayed by RP-HPLC.

用正交设计法优选炮制工艺，采用RP-HPLC法测定松脂醇二葡萄糖苷的含量，作为杜仲质量控制的考察指标进行实验比较。

After 72 h induced by Progesterone and Glucocorticoid as well as Insuline,biological activities of IL-2 were assayed with morphological docimasia method.

结果显示，转染细胞均可表达IL-2，且所表达的IL-2有促进T淋巴细胞转化和淋巴母细胞成熟的活性；转染pcDNA3 OVP—IL2的输卵管上皮细胞表达产物在1：256稀释水平下仍具有促进T淋巴细胞转化和淋巴母细胞成熟的活性。

After 72h induced by Progesterone and Glucocorticoid as well as Insuline, biological activities of IL-2 were assayed with morphological docimasia method. The results showed that the plasmids of pcDNA3-QVP-IL2 and pcDNA3-IL2 could express IL-2, and the IL-2 could augment T cell proliferation.

结果显示，转染细胞均可表达IL-2，且所表达的IL-2有促进T淋巴细胞转化和淋巴母细胞成熟的活性；转染pcDNA3-OVP-IL2的输卵管上皮细胞表达产物在1：256稀释水平下仍具有促进T淋巴细胞转化和淋巴母细胞成熟的活性。

After treating for 3 menstrual cycles, assayed clinical symptom , dysmenorrheal score , clinical traditional Chinese medicine symptom score and serum levels of hemorrheology to contrast clinical therapeutic effect of the two groups.

以3个月经周期为一疗程，通过比较两组病人治疗前后病情疗效、中医证候疗效、痛经症状积分及血液流变学情况的变化结果，对两种方法的临床疗效进行评价。

UPGMA cluster analysis based on Nei's unbiased measures of genetic identity was assayed for five geographical groups of Elymus nutans, indicating that there was a significantly positive correlation between genetic differentiation and geographical habits among the five groups.

对各地理类群基于Nei氏无偏估计的遗传一致度的聚类分析表明，各地理类群间的遗传分化与其所处的地理生态环境具有较高的相关性。

In order to study the physiological basis of dormancy relieving with low temperature in Fritillaria thunbergii,isoperoxidase changes of bud,adaxial epiderm and inner tissues in scale were assayed by means of electrophoresis over the course of dormancy relieving.

中文摘要：为了探讨浙贝母低温解除休眠的生理机制，利用电泳方法研究了芽、鳞片近轴面表皮与鳞片内部组织3个部位在休眠解除过程中过氧化物酶同工酶的变化。

The growth of H. fusiformis was determined by weighting the fresh algae every two days and the photosynthetic oxygen evolution was assayed by a Clark-type oxygen electrode. To determine the contents of chlorophyll a and UV-absorbing compounds, methanol extracts from H. fusiformis were scanned from 250 to 750 nm. The concentration of chlorophyll a was calculated using the equation of Porra and the contents of UVAC per fresh mass were estimated by determining the ratio of UVAC to chlorophyll a according to Dunlap et al and multiplying the ratio of chlorophyll a content to fresh weight of the sample used.

羊栖菜的生长是通过每两天测量一次藻体的湿重来测定的，光合放氧是用Clark型氧电极测定的，为了测定藻体叶绿素a和紫外吸收物质的含量，从250 nm到750 nm对羊栖菜的甲醇提取液进行扫描，叶绿素a的浓度用Porra的公式计算，紫外吸收物质的计算是根据Dunlap的方法先计算紫外吸收物质和叶绿素a的比率，然后乘以每单位藻体叶绿素a的含量。

Methods:Thyroid function,thyroid microsome antibody,and thyroid globulin antibody were determined with radioimmunoassay in 23 CRF complicated by primary hypothyroidism patients,and the serum calcium,phosphorus,potassium, cholesterol, glucose, urea nitrogen, hemoglobulin,and endogenous creatinine clearance rate were also assayed.

用放射免疫法测定23例CRF合并甲减患者的甲状腺功能、甲状腺微粒体抗体、甲状腺球蛋白抗体及检测血清钙、血清磷、血清钾、血清胆固醇、血清肌酐、血糖、尿素氮、血红蛋白、内生肌酐清除率并与31例单纯CRF患者对照进行分析。

Methods Autologous cord blood serum was used to culture cord mesenchymal stem cells. An enzyme digesting method was used to isolate mesenchymal stem cells from the cord. Morphology, immunotyping and hepatogenic differentiation of these cells were assayed.

分离自体脐血清用于后续细胞培养，利用酶消化法从脐带组织中分离培养间充质干细胞，检测其形态和免疫表型，并采用两步法诱导其向肝细胞诱导分化。

Soybean cultivar "Ludou 4" was inoculated with the AM fungus Glomus fasciculatus, soybean cyst nematode Heterodera glycines race 4 and the AM fungus plus SCN. A number of coefficients such as mycorrhizal colonization, SCN infection rate, activities of chitinase and PAL and transcripts of the genes Chibl(Chitinase b1) and PAL5 in soybean roots were assayed.

本研究系统分析了大豆(品种：'鲁豆4')接种AM真菌Glomusfasciculatum和胞囊线虫(SCN，Heterodera glycines)4号生理小种后各处理菌根和线虫侵染率、几丁质酶和苯丙氨酸解氨酶活性及几丁质酶基因Chib1和苯丙氨酸解氨酶基因PAL5转录物的动态变化。

Neither the killing of Mr Zarqawi nor any breakthrough on the political front will stop the insurgency and the fratricidal murders in their tracks.

在对危险的南部地区访问时，他斥责什叶派民兵领导人对中央集权的挑衅行为。

In fact,I've got him on the satellite mobile right now.

实际上 我们已接通卫星可视电话了