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Methods:DNA from 358 patients with prostate cancer were assayed for the number of CAG repeats.

采用分子生物学方法对358例前列腺癌患者AR基因CAG进行测试，分析CAG长度与临床各种指标的关系。

Methods: Normal and benign nodular thyroid samples were assayed for ER and PR with Dextran Carbo Activatus Saturation Analysis.

以葡聚糖活性碳饱和分析法测试人正常甲状腺组织20例良性结节各10例及其雌、孕激素受体的表达。

Blood samples were collected via cardiopuncture and assayed for concentrations of VPA and PHT by FPIA and HPLC method, respectively.

大鼠采交叉设计给药，利用心脏穿刺采血并以萤光免疫分析法及高效液相层析法分别定量血清中之 VPA 及 PHT。

Three methods and 3 calculating formula were used to choose for concrete operation according to the idea at urine iodine assayed with cerous sulfate reducing reaction by which WHO and ICCIDD draw up the preventive and curative criteria of iodine deficiency.

本文根据&WHO与ICCIDD制定的碘缺乏病防治标准与方法&中硫酸铈还原反应测定尿碘的意图，采用3种方法和3种计算公式对具体操作进行选择，并对原标准对照计算法的不合理以及计算误差对评价尿碘水平的效能进行了探讨。结果表明：（1）方法Ⅰ存在仪器误差，准确度不佳。方法Ⅱ线性关系和准确度较理想，但操作不便计算麻烦。方法Ⅲ线性关系和准确度与方法Ⅱ无差异，操作和计算简便、节约试剂。

objective effects of plant tannins,chebulinic acid and tellimagrandin i on chemically induced hemoglobin synthesis in k562 cells were investigated.methods the hemoglobin synthesis situation was assayed with benzidine staining.erythroid antigens glycophorin a expression on the surface of k562 cells was labeled by direct immunofluorescence using fluorescein isothiocyanate-conjugated anti-gpa antibodies.then flow cytometric analysis was performed to detect gpa expression levels on surface of the cells.results both chebulinic acid and tellimagrandin i could inhibit the hemoglobin synthesis of butyric acid and hemin-treated k562 cells in a concentration-dependant manner.however,the ba-induced k562 cells were more sensitive to two tannins than heroin-induced cells.conclusion chebulinic acid and tellimagrandin i have inhibitory effect on the erythroid differentiation.

目的 研究可水解单宁诃子酸和特里马素i对氯化高铁血红素和丁酸钠诱导k562细胞红系分化的影响。方法四甲基偶氮噻唑蓝法分析诃子酸和特里马素i对k562细胞生长的影响，联苯胺染色法检测血红蛋白合成情况，应用免疫荧光和流式细胞术检测血型糖蛋白a在细胞表面的表达。结果诃子酸和特里马素i在0.04～0.09？mmol／l浓度下均可显著抑制k562细胞的生长增殖。2种单宁化合物(0.002～0.01？mmol／l)可显著抑制丁酸钠诱导的k562细胞血红蛋白合成，2种单宁化合物(0.01～0.05？mmol／l)还可显著抑制氯化高铁血红素诱导的血红蛋白合成，特里马素i(0.01？mmol／l)还抑制丁酸钠诱导的gpa在细胞表面表达。结论诃子酸和特里马素i对红系分化有抑制作用。

Methods: CD34(superscript +) cells from fetal liver were isolated with a magnetic cell sorting kit and were cultured. Cells pretreated with or without protein kinase C inhibitor chelerythrine chloride (3 μmol/L) were induced by 5 ×10^(-7)mol/L tretinoin for 24 h, and then incubated in serum-free medium. Expressions of genes in treated cells were assayed by Western blotting and RT-PCR.

采用免疫磁珠法分离人胎肝CD34细胞，培养4d后，蛋白激酶C特异抑制剂chelerythrine chloride(3μmol/L)处理24h，再加入维A酸处理24h5×10^(-7mol/L，无血清培养基培养5d，Western印迹和半定量RT-PCR法分析维A酸处理前后神经特异基因表达。

Chondrogenesis was assayed based on cartilage nodule number and expression of type Ⅱ collagen.

目的 探讨多聚左旋赖氨酸对软骨形成的促进作用及其机制。

The immunological competence of the expressed protein was tested by means of Western blotting and enzyme-linked immunosorbent assay, and its biological activity was assayed by chicken chorioallantoic membrane and Matrigel angiogenesis assay.

酶联免疫吸附和Western blot技术检测纯化的VEGF165蛋白免疫学活性，鸡胚尿囊绒毛膜实验和matrigel血管形成实验检测其生物学活性。

Methods:Fibrinogen was purified by precipitation from citrated plasma by saturated ammonium sulplvate.Fibrinogen-bound sialic acid levels with 22 controls and 113 liver patients were assayed by the thiobarbituric acid method.

应用饱和硫酸铵制备纯化纤维蛋白原，硫代巴比妥酸法测定22例正常人，133例肝病患者血浆FSA水平，并对血浆和纯化纤维蛋白原脱唾液酸化前后的凝血酶时间和纤维蛋白单体聚合浊度进行测定。

In another experiment, cucumber leaves treated with pectinases extract and inoculated with Cladosporium cucumerinum were assayed for these defense-related substances.

以草酸青霉菌的固态发酵产物－果胶酶粗提液喷雾诱导黄瓜，研究了该激发子对黄瓜细胞内几丁质酶和β-1，3-葡聚糖酶2种病程相关蛋白以及细胞壁物质的影响。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。