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DNA methylation level hi stem apices and leaves at different stages of floral bud differentiation were assayed as well.

冬性品种'一点红'在花芽分化初期的可溶性糖含量高于春性品种'短叶13'，但蔗糖和淀粉含量在整个花芽分化期间则始终较低。

Methods 0, 2.5, 5, 10, 15 and 20 μmol/L arsenious acid-treated human hepatocellular carcinoma HepG2 cells were cultured under hypoxia for 8 hours. The cell proliferation and cell viability was assayed by WST-8 and the trypan blue dye exclusion method. The expressions of HIF-1α in human hepatocellular carcinoma HepG2 cells were checked by RT-PCR and Western Blot.

以0、0.25、0.5、1、2和4μmol/L的亚砷酸处理人肝癌细胞HepG2缺氧环境中培养8h；1μmol/L的亚砷酸处理人肝癌细胞HepG2缺氧培养0~10h，WST-8法和台盼蓝染色法分析细胞增殖和活力，采用RT-PCR和Western Blot方法检测肝癌细胞中HIF-1α的表达。

Three combinant retrovial expression vectors, aM1, aM2 and aM3, which was respectively loaded with reverse fragment of c-myc exon 1, 2 and 3, were transferred into rat arterious smooth muscle cells and assayed 1 month after their stable expression.

将分别载有c-myc第1、2和3外显子反义片段的三种重组逆转录病毒表达载体aM1、aM2和aM3导入大鼠主动脉平滑肌细胞，并于持续筛选后1个月进行稳定表达检测。

Methods Three recombinant retroviral expressive vecto rs, aMl, aM2 and aM3, which respectively loading reverse fragment of exon 1,2 and 3, were transferred in to rat arterious SMC and assayed 1 month after their stable expression.

分别载有c-myc 第1、2 和3外显子反义片段的3种重组逆转录病毒表达载体 aM1、aM2 和 aM3 被导入体外培养的大鼠主动脉平滑肌细胞，并于持续筛选后1个月进行稳定表达检测。

The level of PGE was assayed by spectrophotometer; Meanwhile, 3H-TdR incorporation method was used to detect ConA- and LPS- induced splenocytes proliferation and the production of interleukin-1 and IL-2. The content of antibodies to CII was determined by ELISA method. Pathological examination of articulus tissue was observed by Hematoxylin-eosin stain.

检测小鼠足爪肿胀度并对炎症进行评分；测定胸腺指数和脾脏指数；~3H-TdR参入法检测胸腺、脾淋巴细胞增殖反应；~3H-TdR参入法测定白细胞介素-1(interleukin-1，IL-1)和IL-2水平；ELISA法测定小鼠血清中抗CⅡ抗体含量；分光光度法检测足爪局部产生的前列腺素水平；HE染色法对关节组织作病理检查。

Methods The target compounds were synthesized by Stock enamine reaction,Williamson reaction,Mannich reaction,and arylamine exchange reaction with cyclopentanone as starting material,and their antitumor activities were assayed using human hepatic carcinoma cell line(Bel-7402),human oral cavity epidermis squamocellular carcinoma cell l...

方法以环戊酮为原料，经Stock反应、Williamson反应、Mannich反应和芳胺交换反应合成目标化合物。

Methods The UV method for determination of asarone and diprophlline in their mixed solution was established, by which they were assayed when the mixed solution was set at 24 during 6h.

将细辛脑、二羟丙茶碱加入葡萄糖注射液中，混合均匀后，建立细辛脑与二羟丙茶碱混合液紫外分光光度法含量测定方法，分析混合液在室温下(25℃)8 h内2药含量，并观察其pH值、外观、微粒及紫外光谱及峰形变化。

In this paper, Attacus ricini nuclear polyhedrosis virus was studied. The molecular weight and pi of polyhedrin and structural polypeptides were determined by vertical slab SDS-PAGE and thin-layer isoelectrofocusing electrophoresis. The restriction pattern of ArNPV DNA was assayed by 5 restriction endonucleases.

本文较为系统的研究了蓖麻蚕核型多角体病毒(Attacus ricini nuclear polyhedrosis virus，简称ArNPV)形态特征和生化特性，并预测了多角体蛋白的二级结构，为开发利用蓖麻蚕核型多角体病毒这一生物资源提供了理论参考。

Histological differentiation of flower buds in different nodes of new shoots in 6 years old potted Averrhoa carambola was studied. Also carbohydrate contents in these nodes were assayed.

以6年生盆栽甜杨桃为材料，观察了新梢上各节位芽的分化情况，比较了新梢上不同节位的碳水化合物含量。

NO content and NOS activity were assayed with benzidine fluorescence spectrophotometry.

NO含量和NOS活性采用联苯胺荧光分光光度法测定。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。